A5047573274- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- interferon and immune responses
- RNA regulation and disease
- Nanopore and Nanochannel Transport Studies
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- Prion Diseases and Protein Misfolding
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Electrochemical Analysis and Applications
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Telomeres, Telomerase, and Senescence
- Advanced Electron Microscopy Techniques and Applications
- Ion Channels and Receptors
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Biotin and Related Studies
- Molecular Sensors and Ion Detection
- Systemic Lupus Erythematosus Research
- Cholesterol and Lipid Metabolism
University at Buffalo, State University of New York
2021-2025
University of Florida
2015-2025
Laoshan Laboratory
2023-2024
Hauptman-Woodward Medical Research Institute
2021-2023
Marine Biology Institute of Shandong Province
2023
The University of Texas Southwestern Medical Center
2007-2019
Hubei University of Education
2017
Southwestern Medical Center
2009-2015
Rockefeller University
2004-2006
Howard Hughes Medical Institute
2004-2006
Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate cascades, but the underlying structural mechanism unknown. Here we report cryo-electron microscopic structures of helical filaments formed by both N-terminal caspase activation and recruitment domain (CARD) a truncated lacking part proline-rich region C-terminal transmembrane domain. Both are left-handed...
Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between ER cytosolic lipid droplets. Mutations human seipin gene, including missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by lack of normal adipose tissue accumulation fat liver muscles. In both yeast CGL patient fibroblasts, required for droplet morphology; its absence droplets appear bud abnormally from ER. Here we report first purification...
Recent studies hypothesized that phospholipids stabilize two voltage-sensing arginine residues of certain voltage-gated potassium channels in activated conformations. It remains unclear how lipids directly affect these channels. Here, by examining the conformations KvAP different lipids, we showed without voltage change, voltage-sensor domains switched from to resting state when their surrounding were changed nonphospholipids. Such lipid-determined conformational change was coupled...
Significance The ability of synaptotagmin-1 to bridge two membranes is likely critical for its function as a Ca 2+ sensor in neurotransmitter release. A fragment spanning C 2 domains bridges directly, binding simultaneously both membranes. However, longer most cytoplasmic region was proposed through protein–protein interactions between oligomers bound each membrane. This paper now shows that direct bridging actually constitutes the prevalent mechanism membrane by fragment. These findings...
The vanilloid receptor TRPV1 is an exquisite nociceptive sensor of noxious heat, but its temperature-sensing mechanism yet to define. Thermodynamics dictate that this channel must undergo unusually energetic allosteric transition. Thus, it fundamental importance measure directly the energetics transition in order properly decipher mechanism. Previously, using submillisecond temperature jumps and patch-clamp recording, we estimated heat activation for opening incurs enthalpy change on 100...
Defects in normal autophagic pathways are implicated numerous human diseases—such as neurodegenerative diseases, cancer, and cardiomyopathy—highlighting the importance of autophagy its proper regulation. Herein we show that Vibrio parahaemolyticus uses type III effector VopQ (Vibrio outer protein Q) to alter flux by manipulating partitioning small molecules ions lysosome. This binds conserved V o domain vacuolar-type H + -ATPase causes deacidification lysosomes within minutes entering host...
Regulated secretion is conserved in all eukaryotes. In vertebrates granin family proteins function key steps of regulated secretion. Phase separation and amyloid-based storage small molecules secretory granules require ion homeostasis to maintain their steady states, thus need conductances granule membranes. But granular channels are still elusive. Here we show that exocytosis neuroendocrine cells delivers cell surface dominant anion channels, which chromogranin B (CHGB) critical....
Neuronal Munc18-1 and members of the Sec1/Munc18 (SM) protein family play a critical function(s) in intracellular membrane fusion together with SNARE proteins, but mechanism action SM proteins remains highly enigmatic. During experiments designed to address this question employing 7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence de-quenching assay that is widely used study lipid mixing between reconstituted proteoliposomes, we observed from squid (sMunc18-1) was able increase apparent NBD...
Over the past decade, substantial advances have been made in our understanding of transcription factors which regulate gene expression gonadotropes. One most important these factors, steroidogenic factor-1 (SF-1; NR5A1) is critical for gonadotropin and GnRH-receptor expression. Interestingly, a closely related nuclear hormone receptor, liver receptor homologue-1 (LRH-1; NR5A2) has recently detected anterior pituitary gland; however, its functional significance this tissue not investigated....
Fused or giant vesicles, planar lipid bilayers, a droplet membrane system, and planar-supported membranes have been developed to incorporate proteins for the electrical biophysical analysis of such bilayer properties. However, it remains difficult proteins, including ion channels, into reconstituted systems that allow easy control operational dimensions, incorporation orientation composition membranes. Here, using newly chemical engineering procedure, we report on bead-supported unilamellar...