A5081374556- Fungal and yeast genetics research
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Microbial Metabolic Engineering and Bioproduction
- Metabolism, Diabetes, and Cancer
- Bioinformatics and Genomic Networks
- Mitochondrial Function and Pathology
- Gene expression and cancer classification
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Autophagy in Disease and Therapy
- RNA modifications and cancer
Infectious Disease Research Institute
2023-2025
University of Groningen
2014-2018
Dialyse Centrum Groningen
2015
Biotechnology Institute
2015
The nuclear pore complex (NPC) physically interacts with chromatin and regulates gene expression. Saccharomyces cerevisiae inner ring nucleoporin Nup170 has been implicated in organization the maintenance of silencing subtelomeric regions. To gain insight into how this process, we used protein-protein interactions, genetic transcriptome correlation analyses to identify Ctf18-RFC complex, an alternative proliferating cell antigen (PCNA) loader, as a facilitator regulatory functions Nup170. is...
We introduce DeleteomeTools, an R package that leverages the Deleteome compendium of yeast single-gene deletion transcriptomes to predict gene function. Primarily, provides functions for identifying similarities between transcriptomic signatures strains, thereby associating genes interest with others may be functionally related. describe how our software predicted a novel relationship nucleoporin Nup170 and Ctf18-RFC complex, which was confirmed experimentally, revealing previously unknown...
Saccharomyces cerevisiae glycerol phosphate dehydrogenase 1 (Gpd1) and nicotinamidase (Pnc1) are two stress-induced enzymes. Both enzymes predominantly localised to peroxisomes at normal growth conditions, but were reported localise the cytosol nucleus upon exposure of cells stress. Import both proteins into depends on peroxisomal targeting signal 2 (PTS2) receptor Pex7. Gpd1 contains a PTS2, however, Pnc1 lacks this sequence. Here we show that physically interacts with Gpd1, which is...
ABSTRACT Here, we used fluorescence microscopy and a peroxisome-targeted tandem fluorescent protein timer to determine the relative age of peroxisomes in yeast. Our data indicate that yeast cells contain heterogeneous population relatively old young peroxisomes. During budding, peroxisome retention factor inheritance 1 (Inp1) selectively associates older organelles, which are retained mother cells. Inp2, required for transport bud, preferentially younger organelles. Using microfluidics...
Mitochondria are key players in aging and cell death. It has been suggested that mitochondrial fragmentation, mediated by the Dnm1/Fis1 organelle fission machinery, stimulates This was based on observation Saccharomyces cerevisiae Δdnm1 Δfis1 mutants show an enhanced lifespan increased resistance to death inducers. However, machinery is also required for peroxisome division. Here we analyzed significance of yeast chronological lifespan, using strains which mitochondria selectively blocked....
We developed an R codebase that uses a publicly-available compendium of transcriptomes from yeast single-gene deletion strains - the Deleteome to predict gene function. Primarily, provides functions for identifying similarities between transcriptomic signatures strains, thereby associating genes interest with others may be functionally related. describe how our tool predicted novel relationship nucleoporin Nup170 and Ctf18-RFC complex, which was confirmed experimentally, revealing previously...