A5046729908- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Protein Structure and Dynamics
- Heart Failure Treatment and Management
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Adipose Tissue and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Cholesterol and Lipid Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Physiological and biochemical adaptations
- Peroxisome Proliferator-Activated Receptors
- Chemical and Physical Studies
- Peptidase Inhibition and Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Masonry and Concrete Structural Analysis
- biodegradable polymer synthesis and properties
- Cardiovascular Conditions and Treatments
- ATP Synthase and ATPases Research
- Atrial Fibrillation Management and Outcomes
- Neurobiology and Insect Physiology Research
- Heat shock proteins research
University of Graz
2010-2021
BioTechMed-Graz
2015-2021
TU Wien
2021
Saarland University
2018
Goethe University Frankfurt
2015-2018
Universitätsklinikum Würzburg
1987
Cytosolic lipid droplets (LDs) are ubiquitous organelles in prokaryotes and eukaryotes that play a key role cellular organismal homeostasis. Triacylglycerols (TAGs) steryl esters, which stored LDs, typically mobilized growing cells or upon hormonal stimulation by LD-associated lipases ester hydrolases. Here we show the yeast Saccharomyces cerevisiae, LDs can also be turned over vacuoles/lysosomes process morphologically resembles microautophagy. A distinct set of proteins involved LD...
Among the vast variety of Saccharomyces cerevisiae strains, BY family is particularly important because widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum conditions for auxotrophic strains and avoid arrest before glucose depleted. In addition essential supplements required satisfy requirements, found four amino acids phenylalanine, glutamic acid, serine, threonine be...
Yeast Fld1 and Ldb16 resemble mammalian seipin, implicated in neutral lipid storage. Both proteins form a complex at the endoplasmic reticulum-lipid droplet (LD) interface. Malfunction of this either leads to LD clustering or generation supersized (SLD) close vicinity nuclear envelope, response altered phospholipid (PL) composition. We show that similar mutants lacking Fld1, deletion LDB16 abnormal proliferation subdomain which is tightly associated with clustered LD. The human lipin-1...
Highlights•AMPK/Snf1 inhibition of acetyl-CoA carboxylase controls fatty acyl-chain length•Opi1 repressor preferentially binds to C16 rather than C18 acyl-chains in PA•Acyl-chain length tunes Opi1 sequestration the ER and target gene derepression•AMPK/Snf1 thus uses its effect on control genesSummaryMembrane phospholipids typically contain acids (FAs) 16 18 carbon atoms. This particular chain is evolutionarily highly conserved presumably provides maximum stability dynamic properties...
A key event in cellular physiology is the decision between membrane biogenesis and fat storage. Phosphatidic acid (PA) an important intermediate at branch point of these pathways continuously monitored by transcriptional repressor Opi1 to orchestrate lipid metabolism. In this study, we report on mechanism recognition identify amphipathic helix (AH) for selective binding PA over phosphatidylserine (PS). The insertion AH into core renders sensitive acyl chain composition provides a means...
Triacylglycerol (TG) and steryl ester (SE) lipid storage is a universal strategy to maintain organismal energy membrane homeostasis. Cycles of building mobilizing fat are fundamental in (re)distributing substrates between tissues or progress ontogenetic transitions. In this study, we show that Hormone-sensitive lipase (Hsl) specifically controls SE mobilization initiate intergenerational sterol transfer Drosophila melanogaster. Tissue-autonomous Hsl functions the maternal body germline...
Lipid homeostasis is essential for insects to maintain phospholipid (PL)-based membrane integrity and provide on-demand energy supply throughout life. Triacylglycerol (TAG) the major lipid class used production stored in droplets, universal cellular fat storage organelles. Accumulation mobilization of TAG are strictly regulated since excessive accumulation leads obesity has been correlated with adverse effects on health- lifespan across phyla. Little known, however, about when during adult...
Cells respond to protein misfolding and aggregation in the cytosol by adjusting gene transcription a number of post-transcriptional processes. In parallel functional reactions, cellular structure changes as well; however, mechanisms underlying early adaptation compartments cytosolic are less clear. Here we show that mammalian ubiquitin ligase C-terminal Hsp70-interacting (CHIP), if freed from chaperones during acute stress, can dock on membranes thus performing proteostasis sensor function....
Vacuolar ATPases are multisubunit protein complexes that indispensable for acidification and pH homeostasis in a variety of physiological processes all eukaryotic cells. An arginine residue (Arg735) transmembrane helix 7 (TM7) subunit the yeast ATPase is known to be essential proton translocation. However, specific mechanism its involvement transport remains determined. Arginine residues usually assumed "snorkel" toward surface when exposed hydrophobic environment. Here, using solution NMR...
Water, sodium, and potassium balance urinary excretion of norepinephrine aldosterone were investigated in rats with left ventricular failure due to infarction, measurements obtained plasma levels atrial natriuretic factor (ANF). Increased ANF relation the size infarction right end-diastolic pressure found. The augmented not able prevent an accumulation sodium myocardial which was unchanged comparison control values. Plasma pulmonary artery, aorta, renal vein six conscious dogs studied during...
Abstract A key event in cellular physiology is the decision between membrane biogenesis and fat storage. Phosphatidic acid (PA) an important lipid intermediate signaling at branch point of these pathways constantly monitored by transcriptional repressor Opi1 to orchestrate metabolism. Here, we report on mechanism recognition identify amphipathic helix (AH) for selective binding membranes containing PA over phosphatidylserine (PS). The insertion AH into hydrophobic core renders sensitive acyl...
Abstract The article describes the current state of a project examining influences on moisture distribution in cold attics above concrete ceilings residential buildings. Considerable research has been done damages attics, especially Scandinavia and North America, focussing spaces wooden ceilings. (ongoing until Sept 2021) underlying deals with resting masonry walls, frequent variant Austria. Research was triggered by regional Austrian building industry association to shed light onto recent...
Riegger, A. J. G.; Kromer, E. P.; Wild, S.; Hofbauer, H.; Kochsiek, K. Author Information
Water, sodium, and potassium balance urinary excretion of norepinephrine aldosterone were investigated in rats with left ventricular failure due to infarction, measurements obtained plasma levels atrial natriuretic factor (ANF). Increased ANF relation the size infarction right end-diastolic pressure found. The augmented not able prevent an accumulation sodium myocardial which was unchanged comparison control values. Plasma pulmonary artery, aorta, renal vein six conscious dogs studied during...