A5083674919- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Amyotrophic Lateral Sclerosis Research
- Photosynthetic Processes and Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Cholesterol and Lipid Metabolism
- Alzheimer's disease research and treatments
- Muscle metabolism and nutrition
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Lipids, and Metabolism
- Cellular transport and secretion
- Liver Disease Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Microbial Metabolic Engineering and Bioproduction
- Neurogenetic and Muscular Disorders Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Hormonal Regulation and Hypertension
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Hormonal and reproductive studies
- Adipokines, Inflammation, and Metabolic Diseases
Memorial Sloan Kettering Cancer Center
2022-2025
Kettering University
2024
Harvard University
1963-2023
Broad Institute
2015-2023
Boston University
2022
Massachusetts Institute of Technology
2016-2018
Gladstone Institutes
2007-2017
Harvard University Press
2016
University of California, San Francisco
2005-2014
Leibniz Institute of Virology (LIV)
2013
Excess lipid accumulation in non-adipose tissues is associated with insulin resistance, pancreatic β-cell apoptosis and heart failure. Here, we demonstrate cultured cells that the relative toxicity of two common dietary long chain fatty acids related to channeling these lipids distinct cellular metabolic fates. Oleic acid supplementation leads triglyceride well tolerated, whereas excess palmitic poorly incorporated into causes apoptosis. Unsaturated rescue palmitate-induced by palmitate...
Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies sirtuin SIRT3 have so far been limited to cell culture systems. Here, we investigate localization and function vivo. We show that endogenous mouse is a soluble mitochondrial protein. To address relevance regulation energy metabolism, generated phenotypically characterized knockout mice. SIRT3-deficient animals exhibit striking protein hyperacetylation, suggesting major deacetylase....
Monocyte chemoattractant protein-1 (MCP-1) is a potent agonist for mononuclear leukocytes and has been implicated in the pathogenesis of atherosclerosis granulomatous lung disease. To determine role MCP-1 related family members vivo, we used homologous recombination embryonic stem cells to generate mice with targeted disruption C-C chemokine receptor 2 (CCR2), MCP-1. CCR2-/- were born at expected Mendelian ratios developed normally. In response thioglycollate, recruitment peritoneal...
Triacylglycerols are quantitatively the most important storage form of energy for eukaryotic cells. Acyl CoA:diacylglycerol acyltransferase (DGAT, EC 2.3.1.20 ) catalyzes terminal and only committed step in triacylglycerol synthesis, by using diacylglycerol fatty acyl CoA as substrates. DGAT plays a fundamental role metabolism cellular is higher eukaryotes physiologic processes involving such intestinal fat absorption, lipoprotein assembly, adipose tissue formation, lactation. an integral...
Studies involving the cloning and disruption of gene for acyl-CoA:diacylglycerol acyltransferase (DGAT) have shown that alternative mechanisms exist triglyceride synthesis. In this study, we cloned characterized a second mammalian DGAT, DGAT2, which was identified by its homology to DGAT in fungus Mortierella rammaniana. DGAT2 is member family has no with DGAT1 includes several mouse human homologues are candidates additional genes. The expression insect cells stimulated synthesis 6-fold...
The synthesis of triglycerides is catalyzed by two known acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymes. Although they catalyze the same biochemical reaction, these enzymes share no sequence homology, and their relative functions are poorly understood. Gene knockout studies in mice have revealed that DGAT1 contributes to triglyceride tissues plays an important role regulating energy metabolism but not essential for life. Here we show DGAT2 a fundamental mammalian required survival....