A5061602094- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Entomopathogenic Microorganisms in Pest Control
- CRISPR and Genetic Engineering
- Machine Learning in Bioinformatics
- Synthesis and bioactivity of alkaloids
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Lipid metabolism and biosynthesis
- Mycotoxins in Agriculture and Food
- Microtubule and mitosis dynamics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Sphingolipid Metabolism and Signaling
- Plant Reproductive Biology
- Circadian rhythm and melatonin
- Ubiquitin and proteasome pathways
- Dental Trauma and Treatments
- Ginkgo biloba and Cashew Applications
Wichita State University
2023-2024
Toronto Metropolitan University
2018-2023
Maulana Azad Institute of Dental Sciences
2023
Safdarjang Hospital
2023
St. Michael's Hospital
2021-2022
Rai University
2022
Himalayan University
2022
University of Toronto
2018-2021
Unity Health Toronto
2021
The University of Texas at El Paso
2018-2020
Tether proteins attach the endoplasmic reticulum (ER) to other cellular membranes, thereby creating contact sites that are proposed form platforms for regulating lipid homeostasis and facilitating non-vesicular exchange. Sterols synthesized in ER transported by mechanisms plasma membrane (PM), where they represent almost half of all PM lipids contribute critically barrier function PM. To determine whether important both sterol exchange between intermembrane regulation metabolism, we...
Among the vast variety of Saccharomyces cerevisiae strains, BY family is particularly important because widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum conditions for auxotrophic strains and avoid arrest before glucose depleted. In addition essential supplements required satisfy requirements, found four amino acids phenylalanine, glutamic acid, serine, threonine be...
The mechanisms that govern organelle adaptation and remodelling remain poorly defined. endo-lysosomal system degrades cargo from various routes, including endocytosis, phagocytosis, autophagy. For phagocytes, endosomes lysosomes (endo-lysosomes) are kingpin organelles because they essential to kill pathogens process present antigens. During phagocyte activation, endo-lysosomes undergo a morphological transformation, going collection of dozens globular structures tubular network in requires...
Abstract Human CGI-58 (for comparative gene identification-58) and YLR099c, encoding Ict1p in Saccharomyces cerevisiae, have recently been identified as acyl-CoA-dependent lysophosphatidic acid acyltransferases. Sequence database searches for like proteins Arabidopsis (Arabidopsis thaliana) revealed 24 with At4g24160, a member of the α/β-hydrolase family being closest homolog. At4g24160 contains three motifs that are conserved across plant species: GXSXG lipase motif, HX4D acyltransferase...
Fibrillar amyloid beta-protein (Aβ) is the principal component of plaques in brains patients with Alzheimers disease. We have studied effect walnut extract on Aß fibrillization by Thioflavin T fluorescence spectroscopy and electron microscopy. The not only inhibited fibril formation a concentration time- dependent manner but it was also able to defibrillize Aβ preformed fibrils. Over 90% inhibition observed 5 ml methanolic (MEOW) both after 2 3 days incubation. maximum defibrillization...
In our proteome-wide screen, Ysp2 (also known as Lam2/Ltc4) was identified a likely physiologically relevant target of the TOR complex 2 (TORC2)-dependent protein kinase Ypk1 in yeast Saccharomyces cerevisiae. subsequently shown to be one new family sterol-binding proteins located at plasma membrane (PM)-endoplasmic reticulum (ER) contact sites. Here we document that and its paralogue Lam4/Ltc3 are authentic substrates vivo show using genetic biochemical criteria Ypk1-mediated...
Cell growth and division requires the precise duplication of cellular DNA content but also membranes organelles. Knowledge about cell-cycle-dependent regulation membrane storage lipid homeostasis is only rudimentary. Previous work from our laboratory has shown that breakdown triacylglycerols (TGs) regulated in a manner, by activation Tgl4 lipase major cyclin-dependent kinase Cdc28. The lipases Tgl3 are required for efficient cell-cycle progression during G1/S (Gap1/replication phase)...
Previously we identified Lam/GramD1 proteins, a family of endoplasmic reticulum membrane proteins with sterol-binding StARkin domains that are implicated in intracellular sterol homeostasis. Here, show how these exchange molecules membranes. An aperture at one end the domain enables to enter/exit binding pocket. Strikingly, wall pocket is longitudinally fractured, exposing bound solvent. Large-scale atomistic molecular dynamics simulations reveal egress involves widening fracture,...
This case series elaborates on the importance, advantages, and clinical applications of removable partial dentures as prosthetic rehabilitation in pediatric dental patients.Tooth loss children is a measure disease with multiple determinants. There are several potential sequelae result tooth loss. Early treatment follow-up keys to successful young patients missing teeth. It critical that oral started early maintain correct functions. Removable prostheses commonly selected options for because...
O-linked glycosylation, an essential post-translational modification process in Homo sapiens, involves attaching sugar moieties to the oxygen atoms of serine and/or threonine residues. It influences various biological and cellular functions. While or residues within protein sequences are potential sites for not all undergo this modification, underscoring importance characterizing its occurrence. This study presents a novel approach predicting intracellular extracellular glycosylation events...
Abstract The mechanisms that govern organelle adaptation and remodeling remain poorly defined. endo-lysosomal system degrades cargo from various routes including endocytosis, phagocytosis autophagy. For phagocytes, endosomes lysosomes (endo-lysosomes) are kingpin organelles since they essential to kill pathogens process present antigens. During phagocyte activation, endo-lysosomes undergo a morphological transformation, going collection of dozens globular structures tubular network in...
Abstract Ergosterol is a prominent component of the yeast plasma membrane and essential for cell viability. It synthesized in endoplasmic reticulum transported to by nonvesicular mechanisms requiring carrier proteins. Oxysterol‐binding protein homologues StARkin proteins have been proposed function as sterol carriers. Although many these are capable transporting sterols between synthetic lipid vesicles vitro , they not ergosterol transport cells, indicating that may be functionally redundant...
Abstract O- linked glycosylation of proteins is an essential post-translational modification process in Homo sapiens , where the attachment a sugar moiety occurs at oxygen atom serine and/or threonine residues. This plays pivotal role various biological and cellular functions. While or residues protein sequence are potential sites for glycosylation, not all glycosylated. Furthermore, reversible. Hence, it vital importance to characterize if when occurs. We propose multi-layer...
Abstract The mean Q10 value for the mortality of Penicillium notatum spores in water from 50 to 55° was 881. In 1 1.25 per cent solutions phenol 15 40° it 3.4; higher values were obtained 0.75 and 0.875 phenol.
Abstract About 98 per cent of Penicillium notatum spores produced germ tubes in liquid nutrient media within 12 hr. Viability was unaffected by storage distilled water for 20 days at 4°. The sporicidal activity phenol measured determining the percentage germinated before and after contact with solution. Spores were separated on membrane filters from fungicide rapid filtration, incubated Horowitz fluid medium. rate death solutions containing 0.5 to 1.25 followed a first order reaction. values...
Abstract 0·05 to 0·2 per cent phenol in Horowitz medium progressively reduces the oxygen uptake of Penicillium notatum spores. 0·08 causes fungistasis and fungicidal action is evident at 0·15 higher concentrations. Exogenous respiration less sensitive than spore germination. Measurement can not be used for quantitative evaluation action. The concentration exponent effect on −1·5.
Bacterial resistance against traditional antibiotics has posed a major problem nowadays and the phytoconstituents present in medicinal plants potential to combat bacterial infections. Leaves of Adhathoda vasica plant were screened for its property. Antibacterial activity was analyzed by preparing extracts different solvents like ethyl acetate, methanol Distilled water (in increasing polaritry). Results obtained compared with commercial antibiotic Gentamicin (used as control) on gram positive...