A5057966378- Burkholderia infections and melioidosis
- Chemical Looping and Thermochemical Processes
- Brucella: diagnosis, epidemiology, treatment
- Vibrio bacteria research studies
- Bacterial Infections and Vaccines
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- Hepatitis B Virus Studies
- Pneumonia and Respiratory Infections
- Computational Drug Discovery Methods
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Biosensors and Analytical Detection
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Photosynthetic Processes and Mechanisms
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Genomics and Phylogenetic Studies
- Coccidia and coccidiosis research
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
Medical University of Graz
2017-2025
Children's Hospital 2
2023
Institute of Medical Microbiology and Hygiene
2023
Vietnam National University, Hanoi
2023
Children's Hospital
2023
University of Graz
2013-2017
The Gram negative bacterium Vibrio cholerae is the causative agent of secretory diarrheal disease cholera, which has traditionally been classified as a noninflammatory disease. However, several recent reports suggest that V. infection induces an inflammatory response in gastrointestinal tract indicated by recruitment innate immune cells and increase cytokines. In this study, we describe colonization defect double extracellular nuclease mutant immunocompetent mice, not evident neutropenic...
Next-generation whole-genome sequencing is essential for high-resolution surveillance of bacterial pathogens, example, during outbreak investigations or source tracking and escape variant analysis. However, current global bioinformatic bottlenecks a long time to result with standard technologies demand new approaches.
Enteric infections induced by pathogens like Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) remain a massive burden in developing countries with increasing morbidity mortality rates. Previously, we showed that the immunization genetically detoxified outer membrane vesicles (OMVs) derived from V. elicits protective immune response based on generation of O antigen antibodies, which effectively block motility binding to sheathed flagellum. In this study, investigated potential...
ABSTRACT Genomic surveillance of pathogens is essential to trace infections and analyze resistance markers. Core genome multilocus sequence typing (cgMLST) facilitates genomic by simplified analysis standardization. However, its application limited the poor cost-efficiency short-read (SR) sequencing. Oxford Nanopore long-read sequencing (ONT-LR), which allows fast on-site with comparatively low costs, could provide an alternative. Despite ONT-LR raw read accuracy improvement, evidence for...
Haemophilus influenzae is a Gram-negative pathogen colonizing the upper respiratory tract mucosa. H. one of several human-restricted bacteria, which bind to carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) on epithelium leading bacterial uptake by eukaryotic cells. Adhesion CEACAMs thought be mediated outer membrane protein (OMP) P5. However, still bound lacking OMP P5 expression, and soluble CEACAM receptor ectodomains failed P5, when heterologously expressed in...
Burkholderia pseudomallei causes the severe disease melioidosis. Whole-genome sequencing (WGS)-based typing methods currently offer highest resolution for molecular investigations of this genetically diverse pathogen. Still, its routine application in diagnostic laboratories is limited by need high computing power, bioinformatic skills, and variable approaches, with latter affecting results. We therefore aimed to establish validate a WGS-based core genome multilocus sequence (cgMLST) scheme,...
Haemophilus influenzae is a Gram-negative bacillus and frequent commensal of the human nasopharynx. Earlier work demonstrated that in H. type b, l-lactate metabolism associated with serum resistance vivo survival organism. To further gain insight into lactate utilization non-typeable (NTHi) isolate 2019 laboratory prototype strain Rd KW20, deletion mutants dehydrogenase (lctD) permease (lctP) were generated characterized. It shown, apparent KM uptake 20.1μM as determined for KW20. Comparison...
Background Melioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality rates, but under-recognized worldwide. In endemic areas, there great need for simple, low-cost and rapid diagnostic tools. previous study we showed, that protein multiplex array 20 B. pseudomallei-specific antigens detects antibodies in melioidosis patients sensitivity specificity. subsequent the potential of anti-B. pseudomallei antibody detection was confirmed using Hcp1 single...
ABSTRACT The facultative human pathogen Vibrio cholerae transits between the gastrointestinal tract of its host and aquatic reservoirs. V. adapts to different situations by timely coordinated expression genes during life cycle. We recently identified a subclass that are induced at late stages infection. Initial characterization demonstrated some these facilitate transition from environmental conditions. Among uptake systems lacking detailed or correct annotation. In this study, we...
Measuring SARS-CoV-2 neutralizing antibodies after vaccination or natural infection remains a priority in the ongoing COVID-19 pandemic to determine immunity, especially against newly emerging variants. The gold standard for assessing antibody-mediated immunity are cell-based live virus neutralization assays. These assays usually take several days, thereby limiting test capacities and availability of rapid results. In this study, therefore, we developed faster assay, which detects through...
Vacuolar ATPases are multisubunit protein complexes that indispensable for acidification and pH homeostasis in a variety of physiological processes all eukaryotic cells. An arginine residue (Arg735) transmembrane helix 7 (TM7) subunit the yeast ATPase is known to be essential proton translocation. However, specific mechanism its involvement transport remains determined. Arginine residues usually assumed "snorkel" toward surface when exposed hydrophobic environment. Here, using solution NMR...
Most of the current knowledge on Burkholderia pseudomallei-induced inflammasome activation and cell death in macrophages is derived from murine systems. Little known about involved bacterial structures mechanisms primary human macrophages. This particular relevance since as well cells lines differ many aspects activation, including proteins recognition patterns. In this study, we therefore aimed (i) to establish an vitro B. pseudomallei infection model with monocyte-derived single donors...
Abstract Background The epidemiology of melioidosis in Vietnam, a disease caused by the soil bacterium Burkholderia pseudomallei, remains unclear. This study aimed to detect paediatric South Vietnam and describe clinical features geographic distribution. Methods We introduced simple laboratory algorithm for detecting B. pseudomallei from samples at Children's Hospital 2 Ho Chi Minh City July 2015. A retrospective observational children <16 y age with culture-confirmed between 2015...
Background Isolation of the soil bacterium Burkholderia pseudomallei from tropical environments is important to generate a global risk map for man and animals acquire infectious disease melioidosis. There increasing evidence, that currently recommended culture protocol using threonine-basal salt solution with colistin (TBSS-C50) enrichment B. Ashdown agar subsequent subculture lacks sensitivity. We therefore investigated, if otherwise rarely encountered erythritol catabolism might be...
Burkholderia pseudomallei , the etiological agent of melioidosis in humans and animals, often occupies environmental niches infection sites characterized by limited concentrations oxygen. Versatile genomic features enable this pathogen to maintain its physiology virulence under hypoxia, but crucial regulatory networks employed switch from oxygen dependent respiration alternative terminal electron acceptors (TEA) like nitrate, remains poorly understood. Here, we combined a Tn 5 transposon...
Rapid molecular surveillance of SARS-CoV-2 S-protein variants leading to immune escape and/or increased infectivity is utmost importance. Among global bottlenecks for variant monitoring in diagnostic settings are sequencing and bioinformatics capacities. In this study, we aimed establish a rapid user-friendly protocol high-throughput S-gene subsequent automated identification variants. We designed two new primer pairs amplify only the immunodominant part nanopore sequencing. Furthermore,...
Burkholderia mallei, the causative agent of glanders, is a clonal descendant pseudomallei, melioidosis, which has lost its environmental reservoir and restricted host range. Despite limitations in terms sensitivity specificity, complement fixation still official diagnostic test for glanders. Therefore, new tools are needed diagnostics to study B. mallei epidemiology. We recently developed highly sensitive serodiagnostic microarray human melioidosis based on multiplex detection pseudomallei...
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Abstract Purpose. The epidemiology of melioidosis in Vietnam, a disease caused by the soil bacterium Burkholderia pseudomallei , remains unclear . This study aimed to detect pediatric South Vietnam and describe clinical features geographical distribution. Methods. We introduced simple laboratory algorithm for detecting B. from samples at Children’s Hospital 2 Ho Chi Minh City July 2015. culture isolates were confirmed molecular methods. A retrospective observational children aged < 16...
The soil bacterium Burkholderia pseudomallei causes melioidosis, a potentially fatal and greatly underdiagnosed tropical disease. Detection of B. in the environment is important to trace source infections, define risk areas for melioidosis increase clinical awareness. Although polymerase chain reaction (PCR)-based environmental detection provides information, culture pathogen remains essential but still methodological challenge. can catabolize erythritol, metabolic pathway, which otherwise...