A5028777446- Mosquito-borne diseases and control
- Burkholderia infections and melioidosis
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- Brucella: diagnosis, epidemiology, treatment
- Vector-borne infectious diseases
- Computational Drug Discovery Methods
- Clusterin in disease pathology
- Pancreatitis Pathology and Treatment
- Case Reports on Hematomas
- Chemical Looping and Thermochemical Processes
- Galectins and Cancer Biology
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Leptospirosis research and findings
University of Oxford
2020-2021
Mahidol Oxford Tropical Medicine Research Unit
2013-2021
Sappasithiprasong Hospital
2020-2021
Mahidol University
2013-2020
London School of Hygiene & Tropical Medicine
2020
Osaka University
2007-2014
Armed Forces Research Institute of Medical Science
2012
Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain the most distinguishing feature chikungunya fever, diagnosis remains difficult because symptoms are shared by many pathogens, including dengue fever. The present study aimed to develop new immunochromatographic test for detection virus antigen serum. Mice were immunized with isolates from patients Thai East/Central/South African genotype, produce...
Abstract Diabetes mellitus (DM) is a serious global health problem currently affecting over 450 million people worldwide. Defining its interaction with major infections an international public priority. Melioidosis caused by Burkholderia pseudomallei , exemplar pathogen for studying intracellular bacterial infection in the context of DM due to 12‐fold increased risk this group. We characterized immune correlates survival peripheral blood acute melioidosis patients and without highlight...
Abstract Orientia tsutsugamushi is an obligate intracellular bacterium found in Leptotrombidium mites that causes the human disease scrub typhus. A distinguishing feature of O. its extensive strain diversity, yet differences virulence between strains are not well defined nor understood. We sought to determine bacterial drivers pathogenicity by comparing murine infections using seven combined with epidemiological data rank each terms relative virulence. Murine cytokine expression revealed two...
Melioidosis is a major neglected tropical disease with high mortality, caused by the Gram-negative bacterium
Abstract Melioidosis, caused by Burkholderia pseudomallei , is a potentially lethal infection with no licensed vaccine. There little understanding of why some exposed individuals have symptoms, while others rapidly progress to sepsis and death, or diabetes confers increased susceptibility. We prospectively recruited cohort 183 acute melioidosis patients 21 control subjects from Northeast Thailand studied immune parameters in the context survival status presence absence diabetes. HLA-B*46...
Chikungunya fever (CHIKF) is an acute febrile illness caused by a mosquito-borne alphavirus, chikungunya virus (CHIKV). This disease re-emerged in Kenya 2004, and spread to the countries around Indian Ocean. The re-emerging epidemics rapidly regions like India Southeast Asia, it was subsequently identified Europe 2007, probably as result of importation cases. On one hand, neglected diseases has only attracted strong attention during large outbreaks. In 2008-2009, there major outbreak...
Background The majority of dengue patients infected with any serotype virus (DENV) are asymptomatic, but the remainder may develop a wide spectrum clinical symptoms, ranging from mild fever (DF) to severe hemorrhagic (DHF). Severe cases occur more often in who experience secondary infection different serotype. A phenomenon called antibody-dependent enhancement (ADE) has been proposed explain onset these cases, exact mechanism ADE remains unclear. Methodology/Principal Finding Virus...
Background Melioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality rates, but under-recognized worldwide. In endemic areas, there great need for simple, low-cost and rapid diagnostic tools. previous study we showed, that protein multiplex array 20 B. pseudomallei-specific antigens detects antibodies in melioidosis patients sensitivity specificity. subsequent the potential of anti-B. pseudomallei antibody detection was confirmed using Hcp1 single...
Abstract Melioidosis is a neglected tropical disease with an estimated annual mortality rate of 89,000 in 45 countries across regions. The causative agent Burkholderia pseudomallei, gram-negative soil-dwelling bacterium. In Thailand, B. pseudomallei can be found multiple regions, along the low-virulence thailandensis and recently discovered variant (BTCV), which expresses pseudomallei–like capsular polysaccharide. Comprehensive studies human immune responses to variants cross-reactivity are...
Abstract Background In vitro drug susceptibility assay of Plasmodium falciparum field isolates processed “immediate ex vivo ” (IEV), without culture adaption, and tested using histidine-rich protein-2 (HRP-2) detection as an assay, is expedient way to track resistance. Methods From 2005 2010, a HRP-2 in assessed 451 P. obtained from subjects with malaria western northern Cambodia, eastern Thailand, IEV, for 50% inhibitory concentrations (IC 50 ) against seven anti-malarial drugs, including...
Abstract Background Apparent emerging artemisinin-resistant Plasmodium falciparum malaria in Southeast Asia requires development of practical tools to monitor for resistant parasites. Although vitro anti-malarial susceptibility tests are widely used, uncertainties remain regarding interpretation P. field isolate values. Methods Performance parameters the W2 clone (considered artemisinin “sensitive”) were evaluated as a reference HRP-2 immediate ex vivo assay. Variability IC 50 s was...
Background Melioidosis, caused by the flagellated bacterium Burkholderia pseudomallei, is a life-threatening and increasingly recognized emerging disease. Toll-like receptor (TLR) 5 germline-encoded pattern recognition to bacterial flagellin. We evaluated association of nonsense TLR5 genetic variant that truncates with clinical outcomes immune responses in melioidosis. Methodology/Principal findings genotyped c.1174C>T 194 acute melioidosis patients Thailand. Twenty-six (13%) were genotype...
Melioidosis is a life-threatening infectious disease caused by the gram-negative bacillus Burkholderia pseudomallei. An effective vaccine needed, but data on protective immune responses in human melioidosis are lacking. We used ELISA and an antibody-dependent cellular phagocytosis assay to identify major features of antibodies patients with acute Thailand. found that high levels B. pseudomallei-specific IgG2 associated protection against death multivariable logistic regression analysis...
Melioidosis is a neglected tropical disease with high mortality rate. It caused by the Gram-negative, CDC category B select agent Burkholderia pseudomallei (B.ps) that intrinsically resistant to first-line antibiotics. An antibody-based vaccine likely be most effective control measure. Previous studies have demonstrated significant mechanistic roles of antibodies in protection against death animal models, but data from human melioidosis scarce. Herein, we used in-vitro antibody-dependent...
Dengue fever (DF) and dengue hemorrhagic (DHF) are caused by mosquito-borne virus (DENV) infection leading to death in tropical subtropical countries. In Thailand, all 4 serotypes of DENV circulating. The most severe cases DF DHF primarily introduced secondary infections. Epidemiological studies have demonstrated that approximately 20% the primary were DENV-1 -3, while DENV-2 or -4 accounted for less than 3%. For this reason, from infections not been well studied. study, sequence diversity...