We demonstrated nanoscale MRI of metabolism in single isolated mitochondria and within living cells using NV centers diamond.

Abstract Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility excite using focused electron beam (cathodoluminescence; CL) high-resolution localization; and (iii) potential nanoscale sensing. For all these schemes, development of versatile molecular labeling relatively small...

Diamond magnetometry is a quantum sensing method involving detection of magnetic resonances with nanoscale resolution. For instance, T1 relaxation measurements, inspired by equivalent concepts in resonance imaging (MRI), provide signal that to conventional MRI but environment. We use nanodiamonds (between 40 and 120 nm) containing ensembles specific defects called nitrogen vacancy (NV) centers. To perform measurement, we pump the NV center ground state (using laser at 532 observe how long...

Here, we present multifunctional fluorescent nanodiamonds (FNDs) for simultaneous drug delivery and free radical detection. For this purpose, modified FNDs containing nitrogen vacancy (NV) centers with a diazoxide derivative. We found that our particles enter cells more easily are able to deliver cancer into HeLa cells. The were characterized by infrared spectroscopy, dynamic light scattering, secondary electron microscopy. Compared the drug, observe sustained release over 72 h rather than...

Diamond magnetometry makes use of fluorescent defects in diamonds to convert magnetic resonance signals into fluorescence. Because optical photons can be detected much more sensitively, this technique currently holds several sensitivity world records for room temperature measurements. It is orders magnitude sensitive than conventional imaging (MRI) detecting resonances. Here, the diamond detect free radical production single living cells with nanometer resolution experimentally demonstrated....

Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out previous studies, we consider the non-fatal of fluorescent on formation reactive oxygen species (an important stress indicator and potential target...

Abstract Skin equivalents (SE) that recapitulate biological and mechanical characteristics of the native tissue are promising platforms for assessing cosmetics studying fundamental processes. Methods to achieve SEs with well‐organized structure, ideal properties limited. Here, combination melt electrowritten PCL scaffolds cell‐laden Matrigel fabricate SE is described. The scaffold provides structural properties, preventing deformation model. model consists a top layer seeding keratinocytes...

Integrated and fiber-packaged magnetic field sensors with a sensitivity sufficient to sense electric pulses propagating along nerves spatial resolution fine enough resolve their propagation directions will trigger tremendous steps ahead in medical diagnostics research. Nitrogen-vacancy centers diamond are best suitable for such sensing tasks under ambient conditions. Current research on uniting good high is facilitated by scanning or imaging techniques. However, these techniques employ...

Fluorescent nanodiamonds are gaining increasing attention as fluorescent labels in biology view of the fact that they essentially nontoxic, do not bleach, and can be used nanoscale sensors for various physical chemical properties. To fully realize nanosensing potential biological applications, two problems need to addressed: their limited colloidal stability, especially presence salts, ability taken up by cells. We show adsorption a suitably designed recombinant polypeptide address both...

Fluorescent nanodiamonds are frequently used as biolabels. They have also recently been established for magnetic resonance and temperature sensing at the nanoscale level. To properly use them in cell biology, we first to understand their intracellular fate. Here, investigated, time, what happens diamond particles during after division yeast (Saccharomyces cerevisiae) cells. More concretely, our goal was answer question of whether remain mother cells or end up daughter Yeast widely a model...

Free radicals play a key role in the ageing process. However, free are small, reactive and short lived thus challenging to measure. We utilize new technique called diamond magnetometry for this purpose. make use of nitrogen vacancy centers nanodiamonds. Via quantum effect these defects convert magnetic resonance signal into an optical signal. While method is increasingly popular its unprecedented sensitivity physics, we here first time measure living cells. Our signals equivalent T1...

Ultraviolet (UV) radiation is known to cause skin issues, such as dryness, aging, and even cancer. Among UV rays, UVB stands out for its ability trigger problems within cells, including mitochondrial dysfunction, oxidative stress, DNA damage. Free radicals are implicated in these cellular responses, but they challenging measure due their short lifetime limited diffusion range. In our study, we used a quantum sensing technique (T1 relaxometry) involving fluorescent nanodiamonds (FNDs) that...

Free radical generation plays a key role in many biological processes including cell communication, maturation, and aging. In addition, free is usually elevated cells under stress as the case for different pathological conditions. liver tissue, produce radicals when exposed to toxic substances but also, instance, cancer, alcoholic disease cirrhosis. However, are small, short-lived, occur low abundance making them challenging detect especially time resolve, leading lack of nanoscale...

Abstract Fluorescent nanodiamonds (FNDs) can be used as nanoscale magnetic resonance sensors and stable optical labels. As a first step for using FNDs nanosensors inside cells, they have to ingested. Several techniques that improve particle uptake been used. A simple approach based on commercially available liposomes is uptake. Uptake into colon cancer cells (HT‐29 cells) demonstrated. Additionally, it shown the time one facilitate diamond yeast by removing cell wall creating so‐called...

Abstract The nitrogen‐vacancy (NV) center in diamond is a powerful and versatile quantum sensor for diverse quantities. In particular, relaxometry (or T1), can be used to detect magnetic noise at the nanoscale. For experiments with single NV centers analysis of data well established. However, due relatively low brightness reproducibility it beneficial biological use ensembles. While increasing number nanodiamond leads more signal, standardized method extract information from still missing....

Nanomaterials can be identified in high-resolution electron microscopy images using lifetime-selective cathodoluminescence.

Protein analysis of potential disease markers in blood is complicated by the fact that proteins plasma show very different abundances. As a result, high-abundance dominate analysis, which often render low-abundance impossible. Depleting one strategy to solve this problem. Here, we present, for first time, simple approach based on selective binding serum surface nanodiamonds. In our proof-of-principle experiments, were able detect, average, eight are present at concentration 1 ng/mL (instead...

Free radicals play a key role in the ageing process. The strongly debated free radical theory of even states that damage caused by is main cause aging on cellular level. However, are small, reactive and short lived thus challenging to measure. We utilize new technique called diamond magnetometry for this purpose. make use nitrogen vacancy centers nanodiamonds. Via quantum effect these defects convert magnetic resonance signal into an optical signal. While method increasingly popular its...

Copper has several biological functions, but also some toxicity, as it can act a catalyst for oxidative damage to tissues. This is especially relevant in the presence of H2O2, by-product oxygen metabolism. In this study, reactions copper with H2O2 have been investigated spectroscopic techniques. These results were complemented by new quantum sensing technique (relaxometry), which allows nanoscale magnetic resonance measurements at room temperature, and nanomolar concentrations. For purpose,...

Diamond is increasingly popular because of its unique material properties. defects called nitrogen vacancy (NV) centers allow measurements with unprecedented sensitivity. However, to achieve ideal sensing performance NV need be within nanometers from the surface and are thus strongly dependent on local chemistry. Several attempts have been made compare diamond surfaces. due high price crystals shallow centers, a limited number chemical modifications studied. Here, we developed systematic...

Diamond is increasingly popular because of its unique material properties. defects called nitrogen vacancy (NV) centers allow for measurements with unprecedented sensitivity. However, to achieve ideal sensing performance, NV need be within nanometers from the surface and are thus strongly dependent on local chemistry. Several attempts have been made compare diamond surfaces. due high price crystals shallow centers, a limited number chemical modifications studied. Here, we developed...

The nitrogen-vacancy (NV) center in diamond is a powerful and versatile quantum sensor for diverse quantities. In particular, relaxometry (or T1), allows to detect magnetic noise at the nanoscale. While increasing number of NV centers nanodiamond collect more signal, standardized method extract information from experiments such ensembles still missing. this article, we use T1 relaxation curves acquired different concentrations gadolinium ions calibrate optimize entire data processing flow,...

The use of an InGaAs/InGaAs tensile-strained-barrier MQW active layer is interesting approach to achieve polarization-insensitive semiconductor optical amplifiers (SOAs) at the 1.55 /spl mu/m wavelength. This work reports on design and experimental results obtained structures grown by LP-MOCVD characterized for their structural quality. SOA devices made from these layers show a dichroism below 1 dB with signal fiber-to-fiber gains up 20 dB, confirming good electrical quality structure.