A5077970172- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Advanced Fluorescence Microscopy Techniques
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Retinal Development and Disorders
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Hearing, Cochlea, Tinnitus, Genetics
- Cytokine Signaling Pathways and Interactions
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Hemoglobin structure and function
- Immune cells in cancer
- Neurobiology and Insect Physiology Research
Radboud Institute for Molecular Life Sciences
2016-2022
Radboud University Nijmegen
2016-2022
Radboud University Medical Center
2016-2022
University Medical Center
2016-2021
University of Groningen
2018
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2014-2017
University of Göttingen
2010-2017
Universitätsmedizin Göttingen
2014-2015
European Neuroscience Institute Göttingen
2014
Cochlear (Australia)
2014
Resource16 November 2017Open Access Source DataTransparent process Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy Katharina N Richter Department of Neuro- Sensory Physiology, University Göttingen Medical Center, Göttingen, Germany Cluster Excellence Nanoscale Microscopy Molecular Physiology the Brain, Search for more papers by this author Natalia H Revelo orcid.org/0000-0003-2492-1317 J Seitz International Max Planck Research School...
The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result absence suitable labeling tools. We introduce this paper novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels plasma and taken up during endocytosis. It remains attached membranes after fixation permeabilization can therefore be used combination with immunostaining super-resolution microscopy. applied mCLING mammalian-cultured...
Abstract Dendritic cells (DCs) present foreign antigen in major histocompatibility complex (MHC) class I molecules to cytotoxic T a process called cross-presentation. An important step this is the release of from lumen endosomes into cytosol, but mechanism still unclear. In study, we show that reactive oxygen species (ROS) produced by NADPH-oxidase NOX2 cause lipid peroxidation, membrane disrupting chain-reaction, which turn results leakage endosomes. Antigen and cross-presentation were...
Drosophila represents a key model organism for dissecting neuronal circuits that underlie innate and adaptive behavior. However, this task is limited by lack of tools to monitor physiological parameters spatially distributed, central synapses in identified neurons. We generated transgenic fly strains express functional fluorescent reporters targeted either pre- or postsynaptic compartments. Presynaptic Ca2+ dynamics are monitored using synaptophysin-coupled GCaMP3, synaptic transmission red...
Actin plays a critical role during the early stages of pathogenic microbe internalization by immune cells. In this study, we identified key mechanism actin filament tethering and stabilization to surface phagosomes in human dendritic We found that actin-binding protein SWAP70 is specifically recruited nascent binding lipid phosphatidylinositol (3,4)-bisphosphate. Multi-color super-resolution stimulated emission depletion (STED) microscopy revealed cage surrounding formed multiple concentric...
Cells producing cytokines often express the receptor for same cytokine, which makes them prone to autocrine signaling. How cytokine release and signaling are regulated in cell is not understood. In this study, we demonstrate that by exogenous self-synthesized inflammatory interleukin-6 (IL-6) within endosomal compartments acts as a cellular brake limits synthesis of IL-6. Our data show IL-6 internalized dendritic cells signals from containing receptor. Newly synthesized also traffics via...
In autophagy, LC3‐positive autophagophores fuse and encapsulate the autophagic cargo in a double‐membrane structure. contrast, lipidated LC3 (LC3‐II) is directly formed at phagosomal membrane LC3‐associated phagocytosis (LAP). this study, we dissected effects of autophagy inhibitors on LAP. SAR405, an inhibitor VPS34, reduced levels LC3‐II inhibited endosomal acidification bafilomycin A1 chloroquine increased LC3‐II, due to degradation acidic lysosomes. However, while LAP, did not. Finally,...
The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin-5 (Stx5) is essential for Golgi transport. In humans, the STX5 mRNA encodes two isoforms, Stx5 Long (Stx5L) from first starting methionine and Short (Stx5S) an alternative at position 55. this study, we identify a human disorder caused by single missense substitution in second (p.M55V), resulting complete loss of short isoform. Patients suffer early fatal multisystem disease, including severe liver...
Abstract mCLING is a fixable endocytosis marker that can be combined with immunolabeling techniques to study the molecular composition of trafficking organelles. used both in cultured cells and tissue if critical sample preparation steps, such as fixation, are correctly performed. This unit describes protocols for application subsequent processing. We include immunostaining embedding procedures confocal high‐resolution microscopy. © 2016 by John Wiley & Sons, Inc.
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in conventional synapses. However, they are difficult use the inner hair cells of auditory pathway (IHCs), as FM appear penetrate through mechanotransducer channels into cytosol IHCs, masking endocytotic uptake. To solve this problem we applied IHCs dye photo-oxidation technique, which renders electron microscopy markers. Photo-oxidation allowed unambiguous identification labeled organelles, despite...
Traffic between the plasma membrane and endomembrane compartments is an essential feature of eukaryotic cells. The secretory pathway sends cargoes from biosynthetic to membrane. This counterbalanced by a retrograde endocytic route for cell homoeostasis. Cells need adapt rapidly environmental challenges such as reduction pO2 which, however, has not been analysed in relation trafficking detail. Therefore, we determined changes normoxia, hypoxia, after reoxygenation.Membrane was using bulk...
We recently identified a key role for SWAP70 as the tethering factor stabilizing F-actin filaments on surface of phagosomes in human dendritic cells by interacting both with Rho-family GTPases and lipid phosphatidylinositol (3,4)-bisphosphate. In this study, we aimed to investigate whether was general among immune phagocytes. Our data reveal that is recruited early macrophages from mouse. The putative inhibitor sanguinarine blocked phagocytosis polymerization, supporting demonstrated...
Abstract The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin-5 (Stx5) is essential for Golgi transport. In humans, the STX5 mRNA encodes two isoforms, Stx5 Long (Stx5L) from first starting methionine and Short (Stx5S) an alternative at position 55. this study, we identify a human disorder caused by single missense substitution in second (p.M55V), resulting complete loss of short isoform. Patients suffer early fatal multisystem disease, including severe...