A5033626038- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced NMR Techniques and Applications
- Enzyme Structure and Function
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Soil Carbon and Nitrogen Dynamics
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Antioxidants, Aging, Portulaca oleracea
- Analytical Chemistry and Chromatography
- Electron Spin Resonance Studies
- Bacteriophages and microbial interactions
- Soil and Water Nutrient Dynamics
- Antimicrobial Resistance in Staphylococcus
- Protein Hydrolysis and Bioactive Peptides
- Microtubule and mitosis dynamics
- Bacterial biofilms and quorum sensing
- Plant nutrient uptake and metabolism
- Trace Elements in Health
- NMR spectroscopy and applications
- RNA Research and Splicing
Wuhan Institute of Technology
2020-2024
University of Wollongong
2015-2024
Illawarra Health and Medical Research Institute
2015-2023
Qingdao Agricultural University
2021-2023
Shandong Freshwater Fisheries Research Institute
2008-2022
Shenyang Agricultural University
2021
Australian National University
2010-2013
University of Leeds
2011
National University of Ireland, Maynooth
2010
The University of Queensland
2010
Carbamazepine (CBZ), a pharmaceutical compound, has been proposed as an anthropogenic marker to assess water quality due its persistence in conventional treatment plants and widespread presence bodies. This paper presents comprehensive literature review on sources occurrences of CBZ bodies, well toxicological effects regulations the drug. Given documented side human body when taken medicinally, careful monitoring is recommended. residues drinking may provide pathway embryos infants via...
We demonstrate sensitive detection of alpha protons fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, capsid and amyloid fibrils. A set 3D spectra based on block was developed applied sequence-specific backbone aliphatic side-chain resonance assignment using only 500 μg sample. These developments accelerate structural...
Cellular senescence is involved in diverse physiological processes. Accumulation of senescent cells can lead to numerous age-related diseases. Therefore, it great significance develop chemical tools effectively detect and eliminate cells. Till date, a dual functional probe that could has yet been accomplished. Herein, β-gal-activated probe, MB-βgal, based on the methylene blue (MB) fluorophore, was designed In absence β-gal, showed no fluorescence its 1O2 production efficiency suppressed...
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins analyzed without any need deuteration of sample. This approach provides assignments backbone 1H, 15N, 13Cα, 13CO resonances yields information about 1H–1H proximities.
The bacterial phosphotriesterases catalyze hydrolysis of the pesticide paraoxon with very fast turnover rates and are thought to be near their evolutionary limit for this activity. To test whether naturally evolved rate could improved through incorporation unnatural amino acids probe role peripheral active site residues in nonchemical steps catalytic cycle (substrate binding product release), we replaced occurring tyrosine acid at position 309 L-(7-hydroxycoumarin-4-yl)ethylglycine (Hco)...
Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded from nucleolytic attack, preventing intra-strand pairing events and playing many other regulatory roles within the replisome. Recent developments in single-molecule approaches have led to a revised picture of replisome that is much more complex how it retains or recycles protein components. Here, we visualize an vitro reconstituted Escherichia coli recruits SSB relying on two different molecular...
Abstract Bacterial cell division is facilitated by a molecular machine—the divisome—that assembles at mid-cell in dividing cells. The formation of the cytokinetic Z-ring tubulin homologue FtsZ regulated several factors, including divisome component EzrA. Here we describe structure 60-kDa cytoplasmic domain EzrA, which comprises five linear repeats an unusual triple helical bundle. EzrA bent into semicircle, providing protein with potential to interact both N- and C-termini adjacent...
Abstract In Escherichia coli, the DnaB helicase forms basis for assembly of DNA replication complex. The stability at fork is likely important successful initiation and progression. Single-molecule experiments have significantly changed classical model highly stable machines by showing that components exchange with free molecules from environment. However, due to technical limitations, accurate assessments in context are lacking. Using vitro fluorescence single-molecule imaging, we visualise...
Abstract Genome duplication occurs while the template DNA is bound by numerous DNA-binding proteins. Each of these proteins act as potential roadblocks to replication fork and can have deleterious effects on cells. In Escherichia coli, are displaced accessory helicase Rep, a translocase that interacts with replisome. The mechanistic details underlying coordination roadblock removal Rep remain poorly understood. Through real-time fluorescence imaging produced individual E. coli replisomes...
A complex of the three (αεθ) core subunits and β2 sliding clamp is responsible for DNA synthesis by Pol III, Escherichia coli chromosomal replicase. The 1.7 Å crystal structure a between PHP domain α (polymerase) C-terminal segment ε (proofreading exonuclease) shows that attached to at site far from polymerase active site. Both contain clamp-binding motifs (CBMs) interact simultaneously with in polymerization mode replication III. Strengthening both CBMs enables isolation stable αεθ:β2...