A5059536442- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Advanced NMR Techniques and Applications
- Acute Ischemic Stroke Management
- Functional Brain Connectivity Studies
- Alzheimer's disease research and treatments
- Dementia and Cognitive Impairment Research
- NMR spectroscopy and applications
- Electron Spin Resonance Studies
- Protein Structure and Dynamics
- Brain Tumor Detection and Classification
- Magnetic and transport properties of perovskites and related materials
- Nuclear and radioactivity studies
- Electronic and Structural Properties of Oxides
- Organometallic Complex Synthesis and Catalysis
- Memory and Neural Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Soil and Unsaturated Flow
- Magnetism in coordination complexes
- Metal complexes synthesis and properties
- Graphite, nuclear technology, radiation studies
- Memory Processes and Influences
- Diet and metabolism studies
- Muon and positron interactions and applications
- Wildlife Ecology and Conservation
CoorsTek (United States)
2025
Novo Nordisk (United States)
2024
Colorado School of Mines
2021-2023
University of Bristol
2012-2021
Centre National de la Recherche Scientifique
2011-2020
École Normale Supérieure de Lyon
2011-2020
Université Claude Bernard Lyon 1
2011-2020
University of Liverpool
2020
Umeå University
2019
Université du Québec
2019
Using a set of six 1H-detected triple-resonance NMR experiments, we establish method for sequence-specific backbone resonance assignment magic angle spinning (MAS) nuclear magnetic (NMR) spectra 5–30 kDa proteins. The approach relies on perdeuteration, amide 2H/1H exchange, high fields, and high-spinning frequencies (ωr/2π ≥ 60 kHz) yields high-quality data, enabling the use automated analysis. is validated with five examples proteins in different condensed states, including two...
We introduce a new approach to improve structural and dynamical determination of large metalloproteins using solid-state nuclear magnetic resonance (NMR) with 1 H detection under ultrafast magic angle spinning (MAS). The is based on the rapid sensitive acquisition an extensive set 15 N 13 C relaxation rates. system which we demonstrate these methods enzyme Cu, Zn superoxide dismutase (SOD), coordinates Cu ion available either in + (diamagnetic) or 2+ (paramagnetic) form. Paramagnetic...
Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection NMR spectra revealing details secondary tertiary structures medium-sized proteins. The technique was applied to the 153-residue microcrystalline ZnII-loaded superoxide dismutase (ZnII-SOD) fully [2H,13C,15N]-labeled 100 % re-protonated at exchangeable sites. Detailed facts importance specialist readers are published as "Supporting Information". Such...
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins analyzed without any need deuteration of sample. This approach provides assignments backbone 1H, 15N, 13Cα, 13CO resonances yields information about 1H–1H proximities.
Both recognition of familiar objects and pattern separation, a process that orthogonalises overlapping events, are critical for effective memory. Evidence is emerging human separation requires dentate gyrus. Dentate gyrus intimately connected to CA3 where, in animals, an autoassociative network enables recall complete memories underpin object/event recognition. Despite huge motivation treat age-related memory disorders, interaction between subfields difficult investigate due small size...
Abstract Plasma calcitonin, glucagon and parathyroid hormone were measured in patients with acute pancreatitis. calcitonin was not detectable 6 specimens obtained from the hypocalcaemic patients. Plama values similar pancreatitis control subjects unrelated to hypocalcaemia, which even induced by infusion. High or rising levels noted association hypo- normocalcaemia, suggesting that rises maintains plasma calcium within normal limits. was, however, undetectable 8 prolonged hypocalcaemia....
We describe a guidance system for achieving automatic landing of fixed‐wing aircraft in unstructured outdoor terrain, using onboard video cameras. The uses optic flow information sensing and controlling the height above ground, on horizon profile, also acquired by vision stabilizing roll pitch, additionally, if required, control stabilization yaw flight direction. At low heights, when is unreliable, stereo used to guide descent close touchdown. While rate gyro augment attitude one designs,...
Abstract Diatoms are an important group of eukaryotic algae with a curious evolutionary innovation: they sheath themselves in cell wall made largely silica. The cellular machinery responsible for silicification includes family membrane permeases that recognize and actively transport the soluble precursor biosilica, silicic acid. However, molecular basis acid remains obscure. Here, we identify experimentally tractable diatom transporter (SIT) homologues study their structure function vitro ,...
Pseudocontact shifts (PCSs) arise in paramagnetic systems which the susceptibility tensor is anisotropic. PCSs depend upon distance from center and position relative to tensor, they can be used as structural restraints protein structure determination. We show that use of 1H-detected solid-state correlations provides facile rapid detection assignment site-specific PCSs, including resolved 1H a large metalloprotein, Co2+-substituted superoxide dismutase (Co2+-SOD). With only 3 mg sample small...
Doped and mixed oxides are a class of materials with widespread applications, ranging from electronics to energy storage. The precise determination ion valency in these is crucial for understanding their electronic, ionic, magnetic, other properties, as well controlling behaviors during processing microstructure development. In this review, we present magnetometry measurement powerful tool determine position doped oxides. Focus given transition metals dilute concentrations. We discuss the...
In MRI, the coherence lifetime T2 is sensitive to magnetic environment imposed by tissue microstructure and biochemistry in vivo. Here we explore possibility that use of relaxometry may provide information complementary provided diffusion tensor imaging (DTI) ageing healthy controls (HC), Alzheimer's disease (AD) mild cognitive impairment (MCI). MRI metrics were quantified HC patients with MCI AD using multi-echo DTI. We used tract-based spatial statistics (TBSS) evaluate quantitative...
Fatty acid ethyl esters are secondary metabolites that produced during microbial fermentation, in fruiting plants and higher organisms ethanol stress. In particular, volatile medium-chain fatty important flavour compounds impart desirable fruit aromas to fermented beverages, including beer wine. The biochemical synthesis of is poorly understood but likely involves acyl-CoA:ethanol O-acyltransferases. Here, we characterize the enzyme hexanoyl transferase 1 (Eht1) from brewer's yeast...
Background Quantitative T2 and diffusion MRI indices inform about tissue state microstructure, both of which may be affected by pathology before atrophy. Purpose To evaluate the capability volumetric quantitative (qMRI) hippocampus entorhinal cortex (EC) for classification amnestic mild cognitive impairment (aMCI) Alzheimer's disease dementia (ADD). Study type Retrospective cross‐sectional study. Population Consecutive cohorts healthy age‐matched controls (n = 62), aMCI patients 25), ADD...
BACKGROUND: The use of T2 relaxation contrast, as measured by MRI, is particularly commonplace in non-invasive assessment the brain. However, mechanisms and uses brain are still not fully understood. hypothesis that may show anisotropy human b rain was studied at 3 T. refers to variation ordered structures with respect direction applied magnetic field. METHODS: Using a T clinical MRI scanner, we made quantitative multi-contrast spin-echo diffusion tensor imaging (DTI) measurements healthy...
Magnetic resonance imaging (MRI) provides an excellent means of studying tissue microstructure noninvasively since the microscopic environment is imprinted on MRI signal even at macroscopic voxel level. Mesoscopic variations in magnetic field, created by microstructure, influence transverse relaxation time (T2) orientation-dependent fashion (T2 anisotropic). However, predicting effects upon observables challenging and requires theoretical insight. We provide a formalism for calculating T2...
Eine Kombination aus hohem magnetischem Feld (1 GHz) und ultraschneller Rotation um den magischen Winkel (60 kHz) ermöglicht die einfache NMR-spektroskopische Detektion der Sekundär- Tertiärstruktur mittelgroßer Proteine. Die Faltung mikrokristallinen, 153 Aminosäuren bestehenden ZnII-beladenen Superoxid-Dismutase (ZnII-SOD) wurde so bestimmt. Das System war vollständig [2H,13C,15N]-markiert an austauschbaren Zentren zu 100 % reprotoniert. Detailed facts of importance to specialist readers...
The synthesis and manipulation of silicon materials on the nanoscale are core themes in nanotechnology research. Inspiration is increasingly being taken from natural world because biological mineralization results precisely controlled, complex silica structures with dimensions millimeter to nanometer. One fascinating example biomineralization occurs diatoms, unicellular algae that sheath themselves an ornate silica-based cell wall. To harvest environment, diatoms have developed a unique...
ABSTRACT BACKGROUND AND PURPOSE Preterm birth is associated with worse neurodevelopmental outcome, but brain maturation in preterm infants poorly characterized standard methods. We evaluated white matter (WM) of infant brains at term‐equivalent age, as a function gestational age birth, using multimodal magnetic resonance imaging (MRI). METHODS Infants born very (<32 weeks gestation) and late (33‐36 were scanned 3 T diffusion tensor (DTI) T2 relaxometry. MRI data analyzed tract‐based...
The objective of this study was to examine age-dependent changes in both T1-weighted and T2-weighted image contrasts spin-echo T2 relaxation time the human brain during healthy ageing. A total 37 participants between ages 49 87 years old were scanned with a 3 Tesla system, using T1-weighted, weighted quantitative imaging. Contrast intensities values calculated for various regions, including individual hippocampal subfields. T1 contrast-to-noise (CNR) gray:white signal intensity ratio (GWR)...
PbPdO2 is a band semiconductor with gap arising from the filled d8 nature of square-planar Pd2+. We establish that hole doping through Li substitution for Pd in results p-type metallic oxide positive temperature coefficient resistance amounts as small 2 mol % Pd. Furthermore, PbPd1–xLixO2 demonstrates high Seebeck and therefore an thermoelectric material thermopower despite conductivity. Up to 4 found substitute verified by Rietveld refinement neutron diffraction data. At this maximal...