A5088992238- RNA and protein synthesis mechanisms
- Advanced NMR Techniques and Applications
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Crystallography and Radiation Phenomena
- RNA Research and Splicing
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- NMR spectroscopy and applications
- Muon and positron interactions and applications
- Legume Nitrogen Fixing Symbiosis
- Various Chemistry Research Topics
- Superconductivity in MgB2 and Alloys
- Superconducting Materials and Applications
- Bacterial biofilms and quorum sensing
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Microbial Community Ecology and Physiology
- Metabolism and Genetic Disorders
- Biocrusts and Microbial Ecology
Science for Life Laboratory
2018-2023
Stockholm University
2018-2023
University of Konstanz
2015-2018
Université Claude Bernard Lyon 1
2012-2016
École Normale Supérieure de Lyon
2012-2016
Centre National de la Recherche Scientifique
2012-2016
Institut des Sciences Analytiques
2014
University of Pisa
2013
Using a set of six 1H-detected triple-resonance NMR experiments, we establish method for sequence-specific backbone resonance assignment magic angle spinning (MAS) nuclear magnetic (NMR) spectra 5–30 kDa proteins. The approach relies on perdeuteration, amide 2H/1H exchange, high fields, and high-spinning frequencies (ωr/2π ≥ 60 kHz) yields high-quality data, enabling the use automated analysis. is validated with five examples proteins in different condensed states, including two...
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins analyzed without any need deuteration of sample. This approach provides assignments backbone 1H, 15N, 13Cα, 13CO resonances yields information about 1H–1H proximities.
The utilization of ribozyme-based synthetic switches in biotechnology has many advantages such as an increased robustness due to cis regulation, small coding space and a high degree modularity. report endonucleolytic twister ribozymes provides new opportunities for the development advanced tools engineering genetic switches. Here we show that ribozyme is distinguished outstandingly flexible expression platform, which conjugation with three different aptamer domains, enables construction one-...
Significance We have investigated multiple aspects of the Euplotes - Polynucleobacter system, which provides a unique opportunity for study an obligate symbiont with closely related free-living organism that itself possesses peculiarly reduced genome and metabolism. confirmed robustness generality patterns in evolution bacterial symbionts’ genome, adding at same time new elements hypotheses concerning reduction both symbiotic bacteria. argue this system will provide exceptionally useful...
Abstract Despite their roles in controlling many cellular processes, weak and transient interactions between large structured macromolecules disordered protein segments cannot currently be characterized at atomic resolution by X‐ray crystallography or solution NMR. Solid‐state NMR does not suffer from the molecular size limitations affecting NMR, it can applied to molecules different aggregation states, including non‐crystalline precipitates sediments. A solid‐state approach based on high...
Schmale 1H-NMR-Linien werden mithilfe von schneller Drehung im magischen Winkel (60 kHz) für vollständig protonierte Proteinproben festen Zustand erhalten. Die Analyse mittelgroßer mikrokristalliner und nichtkristalliner Proteine gelingt ohne Deuterierung der Probe. Dieser Ansatz ermöglicht die Zuordnung 1H-, 15N-, 13Cα- 13CO-Resonanzen des Rückgrats liefert Informationen über das Vorliegen kleiner 1H-1H-Abstände. Detailed facts of importance to specialist readers are published as...
Besides its primary informational role, the sequence of mRNA (mRNA) including 5'- and 3'- untranslated regions (UTRs), contains important features that are relevant for post-transcriptional translational regulation gene expression. In this work a number bacterial twister motifs characterized both in vitro vivo. The analysis their genetic contexts shows these have potential being transcribed as part polycistronic mRNAs, thus we suggest involvement processing mRNA. Our data show...
Abstract Despite their roles in controlling many cellular processes, weak and transient interactions between large structured macromolecules disordered protein segments cannot currently be characterized at atomic resolution by X‐ray crystallography or solution NMR. Solid‐state NMR does not suffer from the molecular size limitations affecting NMR, it can applied to molecules different aggregation states, including non‐crystalline precipitates sediments. A solid‐state approach based on high...
Abstract Upon nutrient depletion, bacteria stop proliferating and undergo physiological morphological changes to ensure their survival. Yet, how these processes are coordinated in response distinct starvation conditions is poorly understood. Here we compare the cellular responses of Caulobacter crescentus carbon (C), nitrogen (N) phosphorus (P) conditions. We find that DNA replication initiation abundance initiator DnaA are, under all three conditions, regulated by a common mechanism...
Upon nutrient depletion, bacteria stop proliferating and undergo physiological morphological changes to ensure their survival. Yet, how these processes are coordinated in response distinct starvation conditions is poorly understood. Here we compare the cellular responses of Caulobacter crescentus carbon (C), nitrogen (N) phosphorus (P) conditions. We find that DNA replication initiation abundance initiator DnaA are, under all three conditions, regulated by a common mechanism involving...
The ability to regulate DNA replication initiation in response changing nutrient conditions is an important feature of most cell types. In bacteria, triggered by the initiator protein DnaA, which has long been suggested respond nutritional changes; nevertheless, underlying mechanisms remain poorly understood. Here, we report a novel mechanism that adjusts DnaA synthesis availability Caulobacter crescentus . By performing detailed biochemical and genetic analysis dnaA mRNA, identified...
ABSTRACT The ability to regulate DNA replication initiation in response changing nutrient conditions is an important feature of most cell types. In bacteria, triggered by the initiator protein DnaA, which has long been suggested respond nutritional changes, nevertheless underlying mechanisms remain poorly understood. Here, we report a novel mechanism that adjusts DnaA synthesis availability Caulobacter crescentus . By performing detailed biochemical and genetic analysis dnaA mRNA identified...