A5043018632- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Molecular Junctions and Nanostructures
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- RNA Research and Splicing
- Crystallography and molecular interactions
University of Konstanz
2015-2021
The utilization of ribozyme-based synthetic switches in biotechnology has many advantages such as an increased robustness due to cis regulation, small coding space and a high degree modularity. report endonucleolytic twister ribozymes provides new opportunities for the development advanced tools engineering genetic switches. Here we show that ribozyme is distinguished outstandingly flexible expression platform, which conjugation with three different aptamer domains, enables construction one-...
The cyclic dinucleotides (CDNs) c-di-GMP, c-di-AMP, and c-AMP-GMP are widely utilized as second messengers in bacteria, where they signal lifestyle changes such motility biofilm formation, cell wall membrane homeostasis, virulence, exo-electrogenesis. For all known bacterial CDNs, specific riboswitches have been identified that alter gene expression response to the messengers. In addition, CDNs trigger potent immune responses, making them attractive adjuvants therapies. Besides three...
Artificial riboswitches based on ribozymes serve as versatile tools for ligand-dependent gene expression regulation. Advantages of these so-called aptazymes are their modular architecture and the comparably little coding space they require. A variety aptamer-ribozyme combinations were constructed in past 20 years resulting applied diverse contexts prokaryotic eukaryotic systems. Most vivo functional OFF-switches, while ON-switches more advantageous regarding potential applications e.g....
Synthetic riboswitches gain increasing interest for controlling transgene expression in diverse applications ranging from synthetic biology, functional genomics, and pharmaceutical target validation to potential therapeutic approaches. However, existing systems often lack the pharmaceutically suited ligands dynamic responses needed advanced applications. Here we present a series of gene through regulation alternative splicing. Placing 5'-splice site into stem structure tetracycline-sensing...