A5073642264- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Polysaccharides Composition and Applications
- Powder Metallurgy Techniques and Materials
- Injection Molding Process and Properties
- Proteins in Food Systems
- Welding Techniques and Residual Stresses
- Bee Products Chemical Analysis
- Synthesis and Catalytic Reactions
- thermodynamics and calorimetric analyses
- Enzyme Structure and Function
- Advanced Welding Techniques Analysis
- Protein Structure and Dynamics
- Chemical Reactions and Mechanisms
- Advanced materials and composites
- Polysaccharides and Plant Cell Walls
- Advancements in Transdermal Drug Delivery
- Laser and Thermal Forming Techniques
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Rheology and Fluid Dynamics Studies
Martin Luther University Halle-Wittenberg
2016-2022
University of Lübeck
2022
Robert Bosch (Germany)
2015
Paderborn University
2009
A major challenge in cross-linking/mass spectrometry (MS) is targeting carboxyl functions proteins under physiological conditions that do not disturb the protein's conformation. Cross-linking of glutamic acid and aspartic residues will greatly expand scope structural mass spectrometry. We discovered carboxyl-reactive cross-linkers have already been employed for many years cross-linking/MS studies, yet a completely different context. Diazirine-based cross-linkers, such as photomethionine...
Cross-linking combined with mass spectrometry (MS) has evolved as an alternative strategy in structural biology for characterizing three-dimensional structures of protein assemblies and mapping protein–protein interactions. Here, we describe integrated workflow automated identification cross-linked products that is based on the use a tandem (MS/MS) cleavable cross-linker (containing 1,3-bis-(4-oxo-butyl)-urea group, BuUrBu) generating characteristic doublet patterns upon fragmentation. We...
Abstract Combining the properties of a zero‐length cross‐linker with cleavability by tandem mass spectrometry (MS/MS) poses great advantages for protein structure analysis using cross‐linking/MS approach. These include reliable, automated data and possibility to obtain short‐distance information 3D‐structures. We introduce 1,1′‐carbonyldiimidazole (CDI) as an easy‐to‐use commercially available, low‐cost reagent that ideally fulfils these features. CDI bridges primary amines hydroxy groups in...
The chemical cross-linking/mass spectrometry (MS) approach is a growing research field in structural proteomics that allows gaining insights into protein conformations. It relies on creating distance constraints between cross-linked amino acid side chains can further be used to derive structures. Currently, the most urgent task for designing novel cross-linking principles an unambiguous and automated assignment of created products. Here, we introduce homobifunctional, amine-reactive, water...
We have synthesized a homobifunctional amine-reactive cross-linking reagent, containing TEMPO (2,2,6,6-tetramethylpiperidine-1-oxy) and benzyl group (Bz), termed TEMPO-Bz-linker, to derive three-dimensional structural information of proteins. The aim for designing this novel cross-linker was facilitate the mass spectrometric analysis cross-linked products by free radical initiated peptide sequencing (FRIPS). In an initial study, we had investigated fragmentation behavior TEMPO-Bz-derivatized...
Previous studies have shown the benefits of amine-reactive, CID-MS/MS-cleavable cross-linker disuccinimidyl dibutyric urea (DSBU) for structural proteomics via cross-linking/MS (XL-MS). To further facilitate automation XL-MS experiments, we synthesized a deuterated (D12) version DSBU combining advantages MS-cleavable linkers and isotope labeling. The rationale conducting with mixture unlabeled stable isotope-labeled is to obtain characteristic mass differences at MS level indicating...
The chemical cross-linking/mass spectrometry (MS) approach is gaining increasing importance as an alternative method for studying protein conformation and deciphering interaction networks. This study part of our ongoing efforts to develop innovative cross-linking principles a facile efficient assignment cross-linked products. We evaluate two homobifunctional, amine-reactive, MS-cleavable cross-linkers regarding their potential automated analysis introduce the bromine phenylurea (BrPU) linker...
Abstract Combining the properties of a zero‐length cross‐linker with cleavability by tandem mass spectrometry (MS/MS) poses great advantages for protein structure analysis using cross‐linking/MS approach. These include reliable, automated data and possibility to obtain short‐distance information 3D‐structures. We introduce 1,1′‐carbonyldiimidazole (CDI) as an easy‐to‐use commercially available, low‐cost reagent that ideally fulfils these features. CDI bridges primary amines hydroxy groups in...
Chemical cross-linking combined with mass spectrometry (XL-MS) and computational modeling has evolved as an alternative method to derive protein 3D structures map interaction networks. Special focus been laid recently on the development application of cross-linkers that are cleavable by collisional activation they yield distinct signatures in tandem spectra. Building our experiences containing MS-labile urea group, we now present biuret-based, CID-MS/MS-cleavable cross-linker imidodicarbonyl...
Plant sphingolipids (SPLs) are available as oral or topical skin care products in order to restore protective nature due their chemical similarity with human ceramides (CERs). However, successful delivery of glucosylceramide (GlcCER) the viable epidermis is challenging barrier nature. The current study aimed develop and characterize optimized nanoemulsion (NE) nanoemulgel (NEG) formulations for lupin GlcCERs. NE components were screened pseudo-ternary phase diagrams constructed at different...
Abstract Der vorliegende Beitrag beschreibt die Koppelung von Modellen für Scherfließen und Wandgleiten über Wandschubspannung. Ansatz beinhaltet Sonderfälle einer Coulombschen Wandreibung nach Uhland Kapillarlänge konstanten Wandgleitgeschwindigkeit Mooney. Als Beispiel wird das Fließmodell strukturviskose Fluide Ostwald de Waele mit Wandschubspannung verknüpft, im Gleitbereich der Potenz des Druckes abhängt. Die untersuchten Massen den Metallpulverspritzguss verhielten sich rheologisch...
Seit ca. 10 Jahren ist ein Lambert-Eaton Syndrom bekannt, welches als nicht-paraneoplastisches Lambert-Eaton-Syndrom diagnostiziert und zum Zeitpunkt der Vorstellung mit Amifampridine (Firdapse ® ) behandelt wurde.