A5072800752- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Hormonal and reproductive studies
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Cancer Genomics and Diagnostics
- Estrogen and related hormone effects
- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immune Cell Function and Interaction
- Cardiac Fibrosis and Remodeling
- Hematopoietic Stem Cell Transplantation
- 3D Printing in Biomedical Research
- Blood disorders and treatments
- Retinoids in leukemia and cellular processes
- Mathematical Biology Tumor Growth
- FOXO transcription factor regulation
- Metastasis and carcinoma case studies
École Polytechnique Fédérale de Lausanne
2011-2024
Swiss Cancer Center Léman
2022-2024
Swiss Group For Clinical Cancer Research
2022
University of Fribourg
2018-2020
Vall d'Hebron Institut de Recerca
2008-2010
Universitat de Barcelona
2009
Obesity is a strong predictor of poor prognosis in breast cancer, especially postmenopausal women. In particular, tumors obese patients tend to seed more distant metastases, although the biology behind this observation remains poorly understood. To elucidate effects microenvironment on metastatic spread, we ovariectomized C57BL/6 J female mice and fed them either regular diet (RD) or high-fat (HFD) generate diet-induced obesity model. We then studied tumor progression metastasis Py230 EO771...
Abstract Integrins play an important role in haematopoietic stem cell (HSC) maintenance the bone marrow niche. Here, we demonstrate that Periostin (Postn) via interaction with Integrin-αv (Itgav) regulates HSC proliferation. Systemic deletion of Postn results peripheral blood (PB) anaemia, myelomonocytosis and lymphopenia, while number phenotypic HSCs increases marrow. −/− mice recover faster from radiation injury concomitant loss primitive HSCs. show accumulation DNA damage generally...
Breast cancer metastasis is a complex process that depends not only on intrinsic characteristics of metastatic stem cells, but also the particular microenvironment supports their growth and modulates plasticity system. In search for microenvironmental factors supporting cell (CSC) tumour progression to metastasis, we here investigated role matricellular protein transforming factor beta induced (TGFBI) in breast cancer. We crossed MMTV-PyMT model mammary gland tumorigenesis with TgfbiΔ/Δ...
Abstract Understanding the mechanisms that enable cancer cells to metastasize is essential in preventing progression. Here we examine metabolic adaptations of metastasis-initiating (MICs) female breast and how those shape their metastatic phenotype. We find endogenous MICs depend on oxidative tricarboxylic acid cycle fatty usage. Sorting tumor based upon solely mitochondrial membrane potential or lipid storage sufficient at identifying MICs. further identify mitochondrially-generated citrate...
Cancer stem cells (CSCs) are defined by their ability to regenerate a tumor upon transplantation. However, it is not yet clear whether tumors contain single CSC population or different subsets of with mixed capacities for initiating primary and secondary tumors. Using two identification strategies, we studied the overlap between metastatic tumor-initiating (TICs) in MMTV-PyMT model. Our results show that model, Lin−CD90−ALDHhigh retained high potential (TIP) orthotopic transplants, contrast...
Membrane-associated guanylate kinase (MAGUK) with inverted domain structure-1 (MAGI1) is an intracellular adaptor protein that stabilizes epithelial junctions consistent a tumor suppressive function in several cancers of origin. Here we report, based on experimental results and human breast cancer (BC) patients’ gene expression data, MAGI1 highly expressed acts as suppressor estrogen receptor (ER)+/HER2− but not HER2+ or triple negative (TNBC). Within the ER+/HER2− subset, high associates...
ABSTRACT Tissues are organized in cellular niches, the composition and interactions of which can be investigated using spatial omics technologies. However, systematic analyses tissue challenged by scale diversity data. Here we present CellCharter, an algorithmic framework to identify, characterize, compare niches spatially resolved datasets. CellCharter outperformed existing approaches effectively identified across datasets generated different technologies, comprising hundreds samples...
It is well known that estrogens regulate cell cycle progression, but the specific contributions and mechanisms of action estrogen receptor beta (ERbeta) remain elusive.We have analyzed levels ERbeta1 ERbeta2 throughout cycle, as consequences over-expression in human prostate cancer LNCaP line.Both mRNA protein expression increased from G1 to S phase decreased before entering G2/M phase, whereas during phase. was detected both nuclear non-nuclear fractions, found exclusively nucleus....
Abstract Background The human Sex Hormone Binding Globulin (SHBG) gene, located at 17p13.1, comprises, least, two different transcription units regulated by promoters. first unit begins with the exon 1 sequence and is responsible for production of plasma SHBG hepatocytes, while second an alternative sequence, which replaces present in liver transcripts. Alternative translation has only been demonstrated testis transgenic mice containing 11-kb transgene testis. Our goal to further...
Background : It is well known that estrogens regulate cell cycle progression, but the specific contributions and mechanisms of action estrogen receptor beta (ER β ) remain elusive. Methods We have analyzed levels ER 1 2 throughout cycle, as consequences over-expression in human prostate cancer LNCaP line. Results Both mRNA protein expression increased from G1 to S phase decreased before entering G2/M phase, whereas during phase. was detected both nuclear non-nuclear fractions, found...
Abstract Metastatic growth in distant organs is the major cause of cancer mortality. Formation metastasis a multi-step process with several rate-limiting steps. While dissemination tumour cells appears to be an early and frequent event, successful initiation metastatic growth, termed “metastatic colonization,” rather inefficient for many types accomplished only by minority that reach sites. Prevalent target sites are characteristic entities. The inefficiency suggests two possibilities:...
The efficacy of anti-cancer therapies depends on the genomic composition tumor, its microenvironment, spatial organization, and intra-tumor heterogeneity. B-cell lymphomas are a heterogeneous group tumors emerging from B-cells at different stages differentiation exhibiting tumor-specific interactions with tumor microenvironment. Thus, effect drug treatments can be influenced by functional among immune cells. Here, we develop platform to maintain small fragments human lymphoma tissue in...
The efficacy of anti-cancer therapies depends on the genomic composition tumor, its microenvironment, spatial organization, and intra-tumor heterogeneity. B cell lymphomas are a heterogeneous group tumors emerging from cells at different stages differentiation exhibiting tumor-specific interactions with tumor microenvironment. Thus, to measure response therapy in lymphoma, it is critical preserve functional among immune cells. Here, we developed platform maintain small fragments human...
The human sex hormone-binding globulin (SHBG) gene comprises at least 6 different transcription units (TU-1, -1A, -1B, -1C, -1D and -1E), is regulated by no less than promoters. best characterized are TU-1 TU-1A: responsible for producing plasma SHBG, while TU-1A transcribed translated in the testis. Transcription of recently described TU-1B, has been demonstrated prostate tissue cancer cell lines, as well other lines such HeLa, HepG2, HeK 293, CW 9019 imr 32. However, there reported data...
The concept of precision medicine emerged to match targeted therapies with the genomic fingerprint each cancer patient. However, cellular composition and architecture tumor tissues are additional parameters that influence response therapies. B-cell lymphomas a heterogenous group tumors emerging from B cells in different stages differentiation specialized areas lymph nodes. Therefore, order reliably anticipate responses treatment, lymphoma models must preserve spatial organization functional...