A5076925467- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Asthma and respiratory diseases
- Reproductive System and Pregnancy
- Rheumatoid Arthritis Research and Therapies
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Pregnancy and preeclampsia studies
- Adipokines, Inflammation, and Metabolic Diseases
- Food Allergy and Anaphylaxis Research
- Neonatal Respiratory Health Research
- Infant Nutrition and Health
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Diabetes and associated disorders
- Infant Health and Development
- Endometriosis Research and Treatment
- Clostridium difficile and Clostridium perfringens research
- Preterm Birth and Chorioamnionitis
- Lymphoma Diagnosis and Treatment
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
University of Gothenburg
2015-2024
University of Duisburg-Essen
2021
Centre for Inflammation Research
2013-2014
Swedish Academy
2012
Germ-free animal models have demonstrated that commensal bacterial colonization of the intestine induces B cell differentiation and activation. Whether with particular species or groups is associated development during early childhood not known. In a prospective newborn/infant cohort including 65 Swedish children, we examined numbers proportions CD20(+), CD5(+), CD27(+) cells in blood samples obtained at several time points first 3 y life using flow cytometry. Fecal were collected cultured...
Cancer treatment with immune checkpoint inhibition (ICI) can cause immune-related adverse events in the central nervous system (CNS irAE). There are no blood biomarkers to detect CNS irAE. We investigated if concentrations of S100-calcium-binding protein B (S100B) and neurofilament light chain (NfL) be used as for irAE assessed incidence a cohort ICI-treated patients.
Objectives Several studies have indicated that early pet keeping could protect the infant from later allergy development. Here, we investigate if there is a dose-dependent association between cat- and dog-keeping during first year of life subsequent Methods Two cohorts were investigated: cross-sectional questionnaire-based study 7- to 8-year-old children (N = 1029) Mölndal Kiruna, birth-cohort Västra Götaland county clinically evaluated for asthma by paediatricians up age 8–9 years 249). The...
We have recently analyzed the profile of T-cell subtypes based on chemokine receptor expression in blood from untreated early rheumatoid arthritis (ueRA) patients compared to healthy controls (HC). Here, we levels respective chemokines plasma ueRA with those HC. also studied association proportions circulating subsets and clinical disease activity.Peripheral was obtained 43 satisfying ACR 2010 criteria who had not received any disease-modifying anti-rheumatic drugs (DMARD) or prednisolone,...
Abstract The pathogenic role and frequency of T cell subtypes in early rheumatoid arthritis are still unclear. We therefore performed a comprehensive analysis the circulating subtype pattern patients with untreated compared to healthy control subjects. Peripheral blood mononuclear cells were obtained from 26 18 age- sex-matched helper types Th0, Th1, Th2, Th17, Th1/17 nonclassic subsets defined by flow cytometry based on expression chemokine receptors CCR4, CCR6, CXCR3. Regulatory CD25+...
Abstract Children growing up on farms have low rates of allergy, but the mechanism for this protective effect has not been fully elucidated. Short chain fatty acids (SCFAs) produced by gut microbiota may play a role in protection from allergy. We measured fecal SCFA levels samples collected 28 farming and 37 control children over first 3 years life using gas chromatography. Data diet other host factors were recorded allergy was diagnosed at 8 age. Among all children, median propionic butyric...
Abstract Background The majority of CD4 + T helper (Th) cells found in the synovial fluid (SF) patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated Th1 cells. In blood, subsets Th2 and Th17 also but it is unknown if these are present RA SF or how cytokines from affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) RA. Methods We examined proportions Th1, Th2, CXCR3 Th17, Th1Th17, peripheral (TPh) follicular (TFh) paired as well phenotype TPh TFh (...
During pregnancy, the maternal immune system must tolerate developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on MR1 molecule, but their presence function at foetal-maternal interface is not known. We here isolated mononuclear from paired samples of peripheral blood (PB), intervillous (IVB), decidua parietalis (DP) following uncomplicated term...
The maternal part of the placenta, decidua, consists immune cells, decidual stromal and extravillous fetal trophoblasts. In a successful pregnancy, these cell compartments interact to provide an intricate balance between tolerance antimicrobial defense. These processes are still poorly characterized in two anatomically different tissues, basalis parietalis. We examined cells from decidua parietalis term placentas (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Abstract Background Lymphopenia, autoantibodies and activation of the type I interferon (IFN) system are common features in systemic lupus erythematosus (SLE). We speculate whether lymphocyte subset counts affected by pregnancy if they relate to autoantibody profiles and/or IFNα protein SLE pregnancy. Methods Repeated blood samples were collected during from 80 women with 51 healthy controls (HC). Late postpartum obtained 19 SLE. Counts CD4 + CD8 T cells, B cells NK measured flow cytometry....
The gut microbiota provides an important stimulus for the induction of regulatory T (Treg) cells in mice, whether this applies to newborn children is unknown. In Swedish children, Staphylococcus aureus has become a common early colonizer gut. Here, we sought study effects bacterial stimulation on neonatal CD4(+) CD25(+) CD127(low) Treg vitro. proportion circulating and their expression FOXP3, Helios CTLA-4 was examined newborns adults. To evaluate if commensal bacteria could induce cells,...
The gut microbiota is a major stimulus for the immune system, and late acquisition of bacteria and/or reduced complexity flora may delay adaptive maturation. However, it unknown how bacterial colonization pattern in human infants related to T cell activation during early childhood. We followed 65 Swedish children FARMFLORA cohort, from birth up 3 years age. In fecal samples collected at several time points first year life, was investigated with use both 16S rRNA next generation sequencing...
Adverse pregnancy outcomes are more common in women with systemic lupus erythematosus (SLE) compared healthy women, but we lack prognostic biomarkers. Plasma interferon alpha (IFNα) protein levels elevated a subgroup of pregnant SLE, whether this is associated unknown. We investigated the relationship between IFNα, adverse and presence autoantibodies SLE pregnancy.
The intervillous space of the placenta is a part fetal-maternal interface, where maternal blood enters to provide nutrients and gas exchange. Little known about immune cells at this site, which are in direct contact with fetal tissues. We have characterized T cell composition chemokine profile paired peripheral samples from healthy mothers giving birth following term pregnancies. Mucosal-associated invariant (MAIT) effector memory (EM) were enriched compared blood, suggesting that MAIT other...
Neonatal and infant immune responses are characterized by a limited capability to generate protective Ab titers memory B cells as seen in adults. Multiple studies support an immature or even impaired character of umbilical cord blood (UCB) themselves. In this study, we provide comprehensive molecular functional comparison cell subsets from UCB adult peripheral blood. Most have mature, naive phenotype The Ig repertoire is highly variable but interindividually conserved, BCR clonotypes...
The role of FOXP3(+) regulatory T cells in the prevention against sensitization and allergy development is controversial.We followed 65 newborn Swedish children from farming non-farming families birth to 3 years age investigated relation between CD4(+) cell subsets blood samples allergic disease.The proportions CD25(high) , CTLA-4(+) CD25(+) CD45RO(+) HLA-DR(+) CCR4(+) or α4β7(+) within population were examined by flow cytometry at several time-points. Mononuclear isolated stimulated with...
Abstract B cell activating factor (BAFF) is a critical cytokine for maturation of immature cells. In murine lymph nodes, BAFF mainly produced by podoplanin-expressing stromal We have previously shown that circulating levels are maximal at birth, and farmers’ children exhibit higher in cord blood than non-farmers’ children. Here, we sought to investigate whether maternal-derived decidual cells from placenta secrete examine what factors could stimulate this production. found podoplanin...
Abstract Objective Treatment with CTLA-4Ig blocks T-cell activation and is clinically effective in RA. However, it unknown if specific CD4+ subsets blood at baseline predict remission after CTLA-4Ig, or other biological treatments different modes of action, how treatment affects T cells patients untreated early RA (eRA). Methods This study included 60 eRA from a larger randomized trial. They were treated methotrexate combined (abatacept, n = 17), anti-IL6 receptor (tocilizumab, 21) anti-TNF...
Population-based neonatal screening using T-cell receptor excision circles (TRECs) identifies infants with profound T lymphopenia, as seen in cases of severe combined immunodeficiency, and a subgroup 22q11 deletion syndrome (22q11DS).To investigate the long-term prognostic value low levels TRECs newborns 22q11DS.Subjects 22q11DS at birth (22q11Low, N=10), matched subjects normal (22q11Normal, healthy controls (HC, N=10) were identified. At follow-up (median age 16 years), clinical...
ABSTRACT In adults, a majority of FOXP3+ Tregs expresses CTLA-4, and this costimulatory molecule is essential to control the expansion other T cells. However, it remains be investigated whether and/or CTLA-4+ are associated with expression memory markers homing receptors on CD4+ Thus, in prospective newborn-infant cohort study, we examined proportions within CD4+CD25+ cell population fractions cells that expressed CD45RA, CD45RO, HLA-DR, α4β7, CD62L, CCR4 at several time-points during first...
Adiponectin is an adipokine with a modulatory role in metabolism and exerting both anti- pro-inflammatory effects. Levels of adiponectin are increased serum synovial fluid from patients rheumatoid arthritis (RA). able to stimulate the production different factors peripheral blood mononuclear cells (PBMCs) fibroblast-like synoviocytes (FLS) subjects established RA. As circulating levels risk factor for future development RA obesity, we hypothesize that implicated at early stage by initiating...