A5049418386- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Malaria Research and Control
- Mosquito-borne diseases and control
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- SARS-CoV-2 and COVID-19 Research
- NF-κB Signaling Pathways
- Complement system in diseases
- Biosimilars and Bioanalytical Methods
- COVID-19 Clinical Research Studies
- Trypanosoma species research and implications
- Allergic Rhinitis and Sensitization
- Invertebrate Immune Response Mechanisms
- Inflammasome and immune disorders
- Cytomegalovirus and herpesvirus research
- Alzheimer's disease research and treatments
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Toxin Mechanisms and Immunotoxins
- Adipokines, Inflammation, and Metabolic Diseases
- RNA Interference and Gene Delivery
Karolinska Institutet
2020-2024
Karolinska University Hospital
2020-2024
Anocca (Sweden)
2023
University of Duisburg-Essen
2021
German Rheumatism Research Centre
2018
Leibniz Association
2018
Freie Universität Berlin
2016-2017
Max Planck Institute for Infection Biology
2016-2017
Humboldt-Universität zu Berlin
2017
Significance In spite of TNF involvement in the pathogenesis multiple sclerosis (MS), systemic neutralization MS patients was not successful. One possible reasons is that possesses both pathogenic and protective features may be related to TNFR1 versus TNFR2 receptor engagement. This study uncovers one such functions mediated by intrinsic signaling T reg cells. mice bearing humanized genetic loci, ablation restricted cells led reduced capacity control Th17 cell responses, exacerbated...
Natural killer (NK) cells are innate immune that contribute to host defense against virus infections. NK respond severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro and activated patients with disease 2019 (COVID-19). However, by which mechanisms detect SARS-CoV-2-infected remains largely unknown. Here, we show the Non-structural protein 13 of SARS-CoV-2 encodes for a peptide is presented human leukocyte antigen E (HLA-E). In contrast self-peptides, viral prevents binding...
Abstract Genetic and experimental evidence suggests that Alzheimer’s disease (AD) risk alleles genes may influence susceptibility by altering the transcriptional cellular responses of macrophages, including microglia, to damage lipid-rich tissues like brain. Recently, sc/nRNA sequencing studies identified similar activation states in subpopulations macrophages aging degenerating brains other diseased tissues. We collectively refer these microglia peripheral as DLAMs. Using macrophage...
Upregulation of diverse self-antigens that constitute components the inflammatory response overlaps spatially and temporally with emergence pathogen-derived foreign antigens. Therefore, discrimination between these inflammation-associated molecules represents a unique challenge for adaptive immune system. Here, we demonstrate CD8
The generation of high-affinity antibodies against pathogens and vaccines requires the germinal center (GC) reaction, which relies on a complex interplay between specialized effector B CD4 T lymphocytes, GC cells follicular helper (TFH) cells. Intriguingly, several positive key regulators reaction are common for both cell types. Here, we report that transcription factor Bhlhe40 is crucial cell-intrinsic negative regulator affecting sides reaction. In activated cells, was required to restrain...
Neonatal and infant immune responses are characterized by a limited capability to generate protective Ab titers memory B cells as seen in adults. Multiple studies support an immature or even impaired character of umbilical cord blood (UCB) themselves. In this study, we provide comprehensive molecular functional comparison cell subsets from UCB adult peripheral blood. Most have mature, naive phenotype The Ig repertoire is highly variable but interindividually conserved, BCR clonotypes...
Human cytomegalovirus (HCMV) infection induces adaptations in the natural killer (NK)-cell compartment. Expanded subsets of adaptive NK cells display potent effector functions against cellular targets, despite their apparent unresponsiveness to stimulation with classical dendritic cell-derived cytokines interleukin (IL)-12 and IL-18. However, it remains unclear whether have completely lost ability sense inflammation via IL-12 IL-18 or these pro-inflammatory signals can be functionally...
Abstract Background Genetic and experimental evidence strongly implicates myeloid cells in the etiology of AD suggests that AD-associated alleles genes may modulate disease risk by altering transcriptional cellular responses macrophages (like microglia) to damage lipid-rich tissues brain). Specifically, recent single-cell/nucleus RNA sequencing (sc/nRNA-seq) studies identified a transcriptionally distinct state subsets aging or degenerating brains (usually referred as disease- associated...
Abstract Background Allergy to dogs affects around 10% of the population in developed countries. Immune therapy allergic patients with dog allergen extracts has shown limited therapeutic benefit. Methods We established a mouse model allergy by repeatedly administering dander and epithelium via intranasal route. also assessed efficacy recombinant multimeric protein containing Can f 1, 2, 4 6 preventing inflammatory responses extracts. Results Repeated inhalation induced infiltration airways T...
Glycosylphosphatidylinositol (GPI) anchor of
Plasmodium relies on actin-based motility to migrate from the site of infection and invade target cells. Using a substrate-dependent gliding locomotion, sporozoites are able move at fast speed (1-3 μm/s). This minimal set actin regulatory proteins occurs in absence detectable filamentous (F-actin). Here we report an overexpression strategy investigate whether perturbations F-actin steady-state levels affect locomotion host invasion. We selected two vital berghei G-actin-binding proteins,...
The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents promising approach rapidly interrogating gene in primary cells.
Abstract Although γδTCRs were discovered more than 30 yr ago, principles of antigen recognition by these receptors remain unclear and the nature antigens is largely elusive. Numerous studies reported that T cell hybridomas expressing several Vγ1-containing TCRs, including Vγ1Vδ6 TCR γδNKT cells, spontaneously secrete cytokines. This property was interpreted as a self-ligand expressed on hybridoma cells themselves. Here, we revisited this finding using recently developed reporter system live...
Abstract The human infant B cell system is considered premature or impaired. Here we show that most cord blood cells are mature and functional as seen in adults, albeit with distinct transcriptional programs providing accelerated responsiveness to T cell-independent cell-dependent stimulation facilitated IgA class switching. Stimulation drives extensive differentiation into antibody-secreting cells, thereby presumably limiting memory formation. neonatal Ig-repertoire highly variable, but...
<h3>Background</h3> TCR-modified T-cell therapy (TCR-T) is emerging as a treatment option for solid tumours containing mutant KRAS,<sup>1–4</sup> an area of great unmet medical need. We have developed high-precision cell-based analytical platform scalable TCR-T product development. Herein, we describe use the to perform target quantification and generate clinical candidate TCR recognizing 10mer peptide derived from KRAS G12V form presented in HLA-A*11:01. <h3>Methods</h3> HLA-presented...