Tissue engineering plays an important role in the production of skin equivalents for therapy chronic and especially burn wounds. Actually, there exists no (cellularized) equivalent which might be able to satisfactorily mimic native skin. Here, we utilized a laser-assisted bioprinting (LaBP) technique create fully cellularized substitute. The unique feature LaBP is possibility position different cell types exact three-dimensional (3D) spatial pattern. For creation substitutes, positioned...

For the aim of ex vivo engineering functional tissue substitutes, Laser-assisted BioPrinting (LaBP) is under investigation for arrangement living cells in predefined patterns. So far three-dimensional (3D) arrangements single or two-dimensional (2D) patterning different cell types have been presented. It has shown that are not harmed by printing procedure. We now demonstrate first time 3D vital LaBP as multicellular grafts analogous to native archetype and formation these cells. this...

Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem (hMSC) were chosen laser experiments due to their high potential regeneration application possibilities therapy. To evaluate influence LIFT cells, survival rate, proliferation apoptotic activity, DNA damages modifications...

There is a high demand for developing methods to produce more native-like 3D corneal structures. In the present study, we produced cornea-mimicking tissues using human stem cells and laser-assisted bioprinting (LaBP). Human embryonic cell derived limbal epithelial (hESC-LESC) were used as source printing epithelium-mimicking structures, whereas adipose tissue (hASCs) constructing layered stroma-mimicking The development optimization of functional bioinks was crucial step towards successful...

Stem cells are of widespread interest in regenerative medicine due to their capability self-renewal and differentiation, which is regulated by three-dimensional microenvironment. In this study, a computer-aided biofabrication technique based on laser-induced forward transfer (LIFT) used generate grafts consisting mesenchymal stem (MSCs). We demonstrate that (i) laser printing does not cause any cell damage; (ii) laser-printed MSC can be differentiated toward bone cartilage; (iii) LIFT allows...

One of the most promising approaches in tissue engineering is application 3D scaffolds, which provide cell support and guidance initial formation stage. The porosity scaffold internal pore organization influence migration play a major role its biodegradation dynamics, nutrient diffusion mechanical stability. In order to control cellular interactions within scaffold, novel technologies capable producing structures accordance with predefined design are required. two-photon polymerization (2PP)...

Utilization of living cells for therapies in regenerative medicine requires a fundamental understanding the interactions between different and their environment. Moreover, common models based on adherent two-dimensional cultures are not appropriate to simulate complex that occur three-dimensional (3D) cell-microenvironment vivo. In this study, we present computer-aided method printing multiple cell types 3D array using laser-assisted bioprinting. By spots human adipose-derived stem (ASCs)...

Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting cells embedded in a hydrogel environment. In this study, human adipose-derived stem (hASCs) were printed free-scalable grid pattern by means LaBP. We demonstrate that neither proliferation ability nor differentiation behaviour was affected LaBP procedure. Furthermore, differentiated down adipogenic lineage pathway for 10 days. verify quantitative assessments markers resemble cell...

We developed a novel two-photon polymerization (2PP) configuration for fabrication of high-aspect three-dimensional (3D) structures, with an overall height larger than working distance the microscope objective used laser beam focusing into photosensitive material. This method is based on modified optical 2PP setup, where (100× high N.A.), immersion oil and cover glass can be moved together material, resulting in effective higher wider range (WOW-2PP). The proposed technique enables...

Computer assisted biofabrication of fully functional living tissue for regenerative medicine involves generation complex three-dimensional constructs consisting cells and biomaterials. Laser BioPrinting (LaBP) based on laser-induced forward-transfer provides unique possibilities the deposition different biomaterials in a well-defined 3D structure. LaBP can be applied to generate scaffold-free cell systems through layer-by-layer technique by combining solutions with materials that are able...

Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings fundamental aspects 3 D cell-cell and cell-matrix interactions vitro. For predictable printing results, a profound knowledge about effects different processing parameters is essential for realisation cell models well-defined densities. Time-resolved imaging the hydrogel jet dynamics quantitative assessment dependence printed droplet diameter on process...

Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to patient without immunological concerns. 3D printing could enable generation organs for replacement therapies or realization organ-on-chip systems individualized medicine. Printing living was demonstrated with immortalized lines, primary adult different...

Intravascular ultrasound imaging (IVUS) was performed to elucidate the discrepancy between clinical history and angiographic findings measure diameter area of lumen normal left coronary artery in 55 patients who presented with chest pain but had angiograms. The (LCA) scanned a 4.8F, 20 MHz mechanically rotated catheter at 413 sites. Atherosclerotic lesions were identified 72 (17%) sites 25 patients. mean (SD) (range) plaque 5.55 (3.56) mm2 (2-26 mm2) it occupied 28.8 (9.6)% (13-70%) cross...

For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bioprinting. Often, thin metal layer is applied as laser-absorbing material called dynamic release (DRL). This vaporized by focused laser pulses generating vapor pressure that propels forward coated biomaterial. Different lasers with wavelengths from 193 to 1064 nanometer have been used. As DRL gold, silver, or titanium layers The pulse durations were usually in the nanosecond range 1...

Neural progenitor cells generated from human induced pluripotent stem (hiPSCs) are the forefront of ″brain-on-chip″ investigations. Viable and functional hiPSC-derived neuronal networks shaping powerful in vitro models for evaluating normal abnormal formation cortical circuits, understanding underlying disease mechanisms, investigating response to drugs. They therefore represent a desirable instrument both scientific community pharmacological industry. However, culture conditions required...

Background Pathologic studies have revealed that coronary arteries undergo compensatory enlargement in the presence of atherosclerosis. Methods In order to assess artery remodeling vivo, we used intravascular ultrasound examine 46 patients (36 men and 10 women; aged 58.2 ± 6.8 years) with non-calcified plaques. The vessel, lumen, plaque areas atherosclerotic normal proximal distal segments were determined. Results A total 92 analyzed. degree stenosis ranged from 9.2 92.8% (mean 34.1 ±16.9%)...

Computer assisted biofabrication of fully functional living tissue for regenerative medicine involves generation complex three-dimensional constructs consisting cells and biomaterials. Laser BioPrinting (LaBP) based on laser-induced forward-transfer provides unique possibilities the deposition different biomaterials in a well-defined 3D structure. LaBP can be applied to generate scaffold-free cell systems through layer-by-layer technique by combining solutions with materials that are able...

The aim of this study was to compare the morphology stable and unstable coronary lesions using intravascular ultrasound in patients undergoing balloon angioplasty determine whether lesion had any influence on mechanism angioplasty. Thirty three (15 18 unstable) single percutaneous transluminal angio plasty were studied with before after intervention. All examinations, recorded S-VHS video tape, off-line matched sites from point minimum lumen area procedure corresponding site prior...

Bioprinting is seen as a promising technique for tissue engineering, with hopes of one day being able to produce whole organs. However, thick requires functional vascular network, which naturally contains vessels various sizes, down capillaries ~10 µm in diameter, often spaced less than 200 apart. If such tissues are be printed, the vasculature would likely need printed at same time, including capillaries. While there many approaches engineering larger defined manner, small usually arise...

Abstract Currently, different 3D printing techniques are investigated for biomaterials and living cells. An ambitious aim is the of fully functional tissue or organs. Furthermore, manifold applications in biomedical research testing pharmaceuticals cosmetics, printed could be a new method, partly substituting test animals. Here we describe laser-based technique applied arrangement vital cells two three-dimensional patterns engineering. First tissue, tested vitro vivo, cell investigating...

Generation of human neuronal networks by three-dimensional (3D) bioprinting is promising for drug testing and hopefully will allow the understanding cellular mechanisms in brain tissue. The application neural cells derived from induced-pluripotent stem (hiPSCs) an obvious choice, since hiPSCs provide access to unlimited number cell types that could be generated differentiation. questions this regard include which differentiation stage optimal printing such networks, what extent addition...