A5013559202- Nonlinear Optical Materials Studies
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Anesthesia and Neurotoxicity Research
- Laser Material Processing Techniques
- Neuroscience and Neural Engineering
- Laser-Ablation Synthesis of Nanoparticles
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Photochromic and Fluorescence Chemistry
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Luminescence Properties of Advanced Materials
- Additive Manufacturing and 3D Printing Technologies
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Pluripotent Stem Cells Research
- Photopolymerization techniques and applications
- Nanofabrication and Lithography Techniques
- Photonic Crystals and Applications
- Cellular Mechanics and Interactions
- Maritime Transport Emissions and Efficiency
- Blood properties and coagulation
Leibniz University Hannover
2021-2025
Laser Zentrum Hannover
2011-2021
Sechenov University
2016-2021
Semenov Institute of Chemical Physics
2018
Burdenko Neurosurgery Institute
2016-2018
Federal Medical-Biological Agency
2018
Russian Academy of Sciences
2016-2018
Federal Scientific Research Centre Crystallography and Photonics
2016-2018
California Institute for Regenerative Medicine
2018
Opinion Leader Research
2018
A novel method for high-speed fabrication of large scale periodic arrays nanoparticles (diameters 40-200 nm) is developed. This based on a combination nanosphere lithography and laser-induced transfer. Fabricated spherical are partially embedded into polymer substrate. They arranged hexagonal array can be used sensing applications. An optical sensor with the sensitivity 365 nm/RIU figure merit 21.5 in visible spectral range demonstrated.
Two-photon polymerization is an appealing technique for producing microscale devices due to its flexibility in structures with a wide range of geometries as well compatibility materials suitable biomedical applications. The greatest limiting factor widespread use two-photon the slow fabrication times associated line-by-line, high-resolution structuring. In this study, recently developed technology was used produce microstructures by multiple foci, which significantly reduces production time....
Abstract Three-dimensional (3D) rapid prototyping technology based on near-infrared light-induced polymerization of photocurable compositions containing upconversion nanomaterials has been explored. For this aim, the rationally-designed core/shell nanoparticles NaYF 4 :Yb 3+ ,Tm /NaYF , with distinct ultraviolet-emitting lines and unprecedentedly high to ultraviolet conversion efficiency $${\eta }_{{\bf{UC}}}^{({\bf{UV}})}=2{\boldsymbol{ \% }}$$ <mml:math...
In this study, we explore the production of well-defined macroscopic scaffolds with two-photon polymerization (2PP) and their use as neural tissue engineering scaffolds. We also demonstrate that these 3D can be replicated via soft lithography, which increases efficiency. Photopolymerizable polylactic acid (PLA) was used to produce by 2PP lithography. assessed biocompatibility using an SH-SY5Y human neuronal cell line primary cultured rat Schwann cells (of direct relevance repair nerve...
Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds bone tissue engineering. with 150, 200, and 250 μm pore sizes are seeded human marrow stem cells (hBMSCs) adipose derived (hASCs) cultured in osteoinductive control media three weeks. Osteogenic differentiation hASCs hBMSCs formation matrix comparatively analyzed via alkaline phosphatase activity (ALP), calcium quantification, osteocalcin staining scanning electron microscopy (SEM). It observed that 150...
Aim: In this study, the suitability of a mixture containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as novel biocompatible photoinitiator for two-photon polymerization (2PP) processing was investigated. Materials & methods: Polyethylene glycol diacrylate crosslinked using Irgacure® 369, Irgacure 2959 or riboflavin–TEOHA mixture; biocompatibility photopolymer extract solutions subsequently assessed via endothelial cell proliferation assay, viability assay single-cell gel...
Aim: To assess the properties of 3D biodegradable scaffolds fabricated from novel star-shaped poly(D,L-lactide) (SSL) materials for bone tissue regeneration. Materials & methods: The SSL polymer was synthesized using an optimized synthetic procedure and applied scaffold fabrication by two-photon polymerization technique. osteogenic differentiation controlled human adipose-derived stem cells cultured 28 days. with or without murine MSCs were implanted into cranial C57/Bl6 mice. Results:...
Neural progenitor cells generated from human induced pluripotent stem (hiPSCs) are the forefront of ″brain-on-chip″ investigations. Viable and functional hiPSC-derived neuronal networks shaping powerful in vitro models for evaluating normal abnormal formation cortical circuits, understanding underlying disease mechanisms, investigating response to drugs. They therefore represent a desirable instrument both scientific community pharmacological industry. However, culture conditions required...
Fabrication of three-dimensional (3D) fibrin scaffolds with tightly controllable pore sizes and interconnections has been investigated. The were produced using a combination two-photon polymerization (2PP) micromolding techniques. Master structures fabricated by 2PP regenerated in two-step microreplication procedure. Scanning electron optical microscopy observations showed that the exhibited highly porous interconnected structure. Seeding endothelial cells resulted their directed lining...
Biocompatible PEG-containing UCNPs were designed for <italic>in vivo</italic> passive targeting of tumor associated with UCNP efficient accumulation and contrast visualization.
Abstract INTRODUCTION Hyperphosphorylation and aggregation of the microtubule‐associated protein tau cause development tauopathies, such as Alzheimer's disease frontotemporal dementia (FTD). We recently uncovered a causal link between constitutive serotonin receptor 7 (5‐HT7R) activity pathological aggregation. Here, we evaluated 5‐HT7R inverse agonists novel drugs in treatment tauopathies. METHODS Based on structural homology, screened multiple approved for their agonism toward 5‐HT7R....
Abstract In recent years, nanotechnology has had a profound impact on low-temperature preservation, offering advanced solutions that significantly improve the storage of biological samples. Low-temperature preservation is crucial for numerous fields, such as medicine, agriculture, and research, where maintaining viability integrity cells, tissues, organs over extended periods essential. Integration into protocols represents significant advancement, enabling more effective, safer, efficient...
Zr-Si organic–inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth synaptogenesis confirmed immunohistochemical staining with antibodies against βIII-Tubulin synaptophysin. The formation functional network was assessed measurement spontaneous activity using...
Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve properties remains undiscussed despite their importance,<italic>e.g.</italic>in designing biomaterials that ensure cell migration and lumenogenesis.
One of the essential goals in regenerative medicine is microvascularization which enables an effective blood supply within de novo constructed tissues and organs. In our study, we used two common multipotent mesenchymal stromal cell (MMSC) sources (subcutaneous adipose tissue Wharton's jelly umbilical cord) where a subpopulation endothelial precursors. medium supplemented with VEGF, 3D cultures UC MMSCs ADSCs promoted differentiation. To evaluate their ability to form capillary-like network,...
Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to efficient production neuronal cell models for vitro study. This potential enable understanding live cellular and network function which is otherwise not possible. However, a major challenge generation reproducible neural networks together with ability interrogate record at single level. A promising aid use biomaterial scaffolds that would development guidance physiologically relevant architectures...
The fabrication of defined surface topographies and three-dimensional structures is a challenging process for various applications, e.g. in photonics biomedicine. Laser-based technologies provide promising approach the production such structures. advantages femtosecond laser ablation two-photon polymerization microstructuring are well known. However, these methods cannot be applied to all materials limited by their high cost long time. In this study, biomedical applications an indirect rapid...
In the presented study, we have developed a synthetic strategy allowing gradual variation of polylactide arms' length, which later influences micromorphology scaffold surface, formed by two-photon polymerization technique. It has been demonstrated that highest number cells is present on scaffolds with roughest surface made longer arms (PLA760), and osteogenic differentiation mesenchymal stem most pronounced such scaffolds. According to results biological testing, PLA760 were implanted into...
Abstract In this work, we have explored 3-D co-culture of vasculogenic cells within a synthetically modified fibrin hydrogel. Fibrinogen was covalently linked with PEG-NHS in order to improve its degradability resistance and physico-optical properties. We studied influences the degree protein PEGylation concentration enzyme thrombin used for gel preparation on cellular responses. Scanning electron microscopy analysis prepared gels revealed that strongly influenced microstructural...
Two-photon polymerization is a technique that involves simultaneous absorption of two photons from femtosecond laser for selective photosensitive material. In this study, two-photon was used layer-by-layer fabrication 3-D scaffolds composed an inorganic–organic zirconium oxide hybrid Four types scaffold microarchitectures were created, which exhibit layers parallel line features at various orientations as well pores between the features. Long-term cell culture studies involving human bone...