A5063359135- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Cancer, Hypoxia, and Metabolism
- Nerve injury and regeneration
- Mitochondrial Function and Pathology
- High Altitude and Hypoxia
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Biochemical effects in animals
- Nanoplatforms for cancer theranostics
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Cardiovascular Disease and Adiposity
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Neonatal and fetal brain pathology
- Graphene and Nanomaterials Applications
- Bone Tissue Engineering Materials
- Autophagy in Disease and Therapy
- Neurological Disorders and Treatments
N. I. Lobachevsky State University of Nizhny Novgorod
2015-2024
Privolzhsky Research Medical University
2012-2020
Yaroslav-the-Wise Novgorod State University
2012-2020
Saratov State University
2020
Samara State Medical University
2017
<h3>Background</h3> Anti-cancer therapy is more successful when it can also induce an immunogenic form of cancer cell death (ICD). Therefore, developing new treatment strategies, extremely important to choose methods that ICD and thereby activate anti-tumor immune response leading the most effective destruction tumor cells. The aim this work was analyze whether clinically widely used photosensitizers, photosens (PS) photodithazine (PD), in photodynamic (PDT). <h3>Methods</h3> Cell murine...
The extracellular matrix (ECM) plays an important role in use-dependent synaptic plasticity. Hyaluronic acid (HA) is the backbone of neural ECM, which has been shown to modulate α-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA) receptor mobility, paired-pulse depression, L-type voltage-dependent Ca(2+) channel (L-VDCC) activity, long-term potentiation and contextual fear conditioning. To investigate HA development spontaneous neuronal network we used microelectrode array recording...
Understanding the molecular underpinnings of neurodegeneration processes is a pressing challenge for medicine and neurobiology. Alzheimer’s disease (AD) Parkinson’s (PD) represent most prevalent forms neurodegeneration. To date, substantial body experimental evidence has strongly implicated hypoxia in pathogenesis numerous neurological disorders, including AD, PD, other age-related neurodegenerative conditions. Hypoxia-inducible factor (HIF) transcription that triggers cell survival program...
The neuroprotective and antihypoxic effects of brain-derived neurotrophic factor (BDNF) on dissociated hippocampal cultures in a hypoxia model were investigated. These experiments demonstrate that 10 minutes normobaric increased the number dead cells primary culture, whereas preventive application BDNF viable cells. Spontaneous bioelectrical calcium activity neural networks was analyzed using multielectrode arrays functional intravital imaging. results indicate affects parameters neuronal...
Zr-Si organic–inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth synaptogenesis confirmed immunohistochemical staining with antibodies against βIII-Tubulin synaptophysin. The formation functional network was assessed measurement spontaneous activity using...
Glial cell line-derived neurotrophic factor (GDNF) has a pronounced neuroprotective effect in various nervous system pathologies, including ischaemic brain damage and neurodegenerative diseases. In this work, we studied the of GDNF on ultrastructure functional activity neuron-glial networks during acute hypoxic exposure, key damaging numerous pathologies. We analysed molecular mechanisms most likely involved positive effects GDNF. Hypoxia modelling was performed day 14 culturing primary...
Abstract Photodynamic therapy (PDT) is a clinically approved procedure for targeting tumor cells. Though several different photosensitizers have been developed, there still much demand novel with improved properties. In this study we aim to characterize the accumulation, localization and dark cytotoxicity of developed in‐house derivatives porphyrazines ( pz I‐IV) in primary murine neuronal cells, as well identify concentrations at which effectively induces death glioma cells yet nontoxic...
Discovering the mechanisms underlying homeostatic regulation in brain neural network formation and stability processes is one of most urgent tasks modern neuroscience. Brain-derived neurotrophic factor (BDNF) tropomyosin-related kinase B (TrkB) receptor system have long been considered main regulators neuronal survival differentiation. The elucidation methods for studying activity makes investigating complex structure reorganization during development detecting new remodeling possible. In...
A novel potent analog of the branched tail oxyquinoline group hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors, neuradapt, has been studied in two treatment regimes an vitro hypoxia model on murine primary hippocampal cultures. Neuradapt activates expression HIF1 and HIF2 target genes shows no toxicity up to 20 μM, which is more than order magnitude higher its biologically active concentration. Cell viability, functional activity, network connectivity between elements neuronal...
Alzheimer's disease (AD) is a widespread chronic neurodegenerative pathology characterized by synaptic dysfunction, partial neuronal death, cognitive decline and memory impairments. The major hallmarks of AD are extracellular senile amyloid plaques formed various types proteins (Aβ) the formation accumulation intracellular neurofibrillary tangles. However, there lack relevant experimental models for studying changes in neural network activity, features intercellular signaling or effects...
Ischemic brain injury is a widespread pathological condition, the main components of which are deficiency oxygen and energy substrates. In recent years, number new forms cell death, including necroptosis, have been described. cascade interactions between kinases RIPK1 RIPK3 MLKL protein leads to formation specialized death complex called necrosome, triggers MLKL-mediated destruction membrane necroptotic death. Necroptosis probably plays an important role in development ischemia/reperfusion...
Glial cell line-derived neurotrophic factor (GDNF) is regarded as a potent neuroprotector and corrector of neural network activity in stress conditions. This work aimed to investigate the effect GDNF on primary hippocampal cultures during acute normobaric hypoxia. Hypoxia induction was performed using day 14 vitro derived from mouse embryos (E18) with preventive addition (1 ng/ml) culture medium 10 min before oxygen deprivation. An analysis spontaneous bioelectrical that included defining...
Brain-derived neurotrophic factor (BDNF) is one of the key signaling molecules that supports viability neural cells in various brain pathologies, and can be considered a potential therapeutic agent. However, several methodological difficulties, such as overcoming blood–brain barrier short half-life period, challenge use BDNF clinical practice. Gene therapy could overcome these limitations. Investigating influence viral vectors on network level particular interest because overexpression...
The research demonstrated that upconversion nanoparticles (UCNPs) are toxic to nervous cells. cytotoxic severity depends on surface modification of UCNPs.
Whether and under what conditions astrocytes can mount a collective network response has recently become one of the central questions in neurobiology. Here, we address this problem, investigating astrocytic reactions to different biochemical stimuli ischemic-like vitro. Identifying an emergent is based on novel mathematical approach that extracts calcium activity from time-lapse fluorescence imaging estimates connectivity astrocytes. The developed algorithm represents as oriented graph which...
Accumulated experimental data strongly suggest that astrocytes play an important role in the pathogenesis of neurodegeneration, including Alzheimer’s disease (AD). The effect on calcium activity neuron–astroglia networks AD modelling was object present study. We have expanded and improved our approach’s capabilities to analyze activity. developed a novel algorithm construct dynamic directed graphs both astrocytic neuronal networks. proposed allows us not only identify functional...
Optogenetics is a combination of optical and genetic technologies used to activate or, conversely, inhibit specific cells in living tissues. The possibilities using optogenetics approaches for the treatment epilepsy, Parkinson’s Alzheimer’s disease (AD) are being actively researched. In recent years, it has become clear that one most important players development AD astrocytes. Astrocytes affect amyloid clearance, participate neuroinflammation, regulate functioning neural networks. We an...