A5042379176- Enzyme-mediated dye degradation
- Electrochemical sensors and biosensors
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Electrochemical Analysis and Applications
- bioluminescence and chemiluminescence research
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Biochemical and biochemical processes
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Biochemical Acid Research Studies
- Epigenetics and DNA Methylation
- Plant Stress Responses and Tolerance
- Glutathione Transferases and Polymorphisms
- Free Radicals and Antioxidants
- Trace Elements in Health
- Advanced Nanomaterials in Catalysis
- Enzyme Catalysis and Immobilization
- Adipose Tissue and Metabolism
- Photoreceptor and optogenetics research
- Analytical Chemistry and Sensors
- Analytical chemistry methods development
Pace University
2006-2024
Lomonosov Moscow State University
2015-2024
National Research University Higher School of Economics
2021-2024
Physical Sciences (United States)
2008-2024
A N Bach Institute of Biochemistry
2022
New York Medical College
2016-2021
P.A. Hertzen Moscow Oncology Research Institute
2020-2021
Ministry of Health of the Russian Federation
2020-2021
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
2016-2018
Burke Medical Research Institute
2006-2018
A promising approach to neurotherapeutics involves activating the nuclear-factor-E2-related factor 2 (Nrf2)/antioxidant response element signaling, which regulates expression of antioxidant, anti-inflammatory, and cytoprotective genes. Tecfidera, a putative Nrf2 activator, is an oral formulation dimethylfumarate (DMF) used treat multiple sclerosis. We compared effects DMF its bioactive metabolite monomethylfumarate (MMF) on signaling their ability block...
Damaging interactions between antibodies and brain antigenic targets may be responsible for an expanding range of neurological disorders. In the case systemic lupus erythematosus (SLE), patients generate autoantibodies (AAbs) that frequently bind dsDNA. Although some symptoms SLE arise from direct reactivity to dsDNA, much AAb-mediated damage originates cross-reactivity with other antigens. We have studied AAbs dsDNA cross-react NR2A NR2B subunits NMDA receptor (NMDAR). adult mouse models,...
Recent observations point to the role played by Zn2+ as an inducer of neuronal death. Two targets have been identified that result in inhibition mitochondrial respiration: bc1 center and, more recently, α-ketoglutarate dehydrogenase. is also a mediator oxidative stress, leading failure, release apoptotic peptides, and We now present evidence, means direct biochemical assays, imported through Ca2+ uniporter directly major enzymes energy production (lipoamide dehydrogenase) antioxidant defense...
Although the etiology of Parkinson's disease (PD) remains unclear, ample empirical evidence suggests that oxidative stress is a major player in development PD and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity. Nuclear factor E2-related 2 (Nrf2) redox-sensitive transcription upregulates battery antioxidant response element (ARE)-driven antioxidative cytoprotective genes defend against stress.We evaluated whether strategy activation Nrf2 its downstream network with small...
Neurons rely on their metabolic coupling with astrocytes to combat oxidative stress. The transcription factor nuclear erythroid 2-related 2 (Nrf2) appears important for astrocyte-dependent neuroprotection from insults. Indeed, Nrf2 activators are effective in stroke, Parkinson disease, and Huntington disease models. However, key endogenous signals that initiate adaptive neuroprotective cascades astrocytes, including activation of Nrf2-mediated gene expression, remain unclear. Hydrogen...
Blocking oxygen-sensing prolyl hydroxylases in the rodent CNS enhances functional recovery after brain hemorrhage.
Targeting newly identified damage pathways in the ischemic brain can help to circumvent currently severe limitations of acute stroke therapy. Here we show that activity 12/15-lipoxygenase was increased mouse brain, and colocalized with a marker for oxidized lipids, MDA2. This colocalization also detected 2 human patients, where it coincided apoptosis-inducing factor. A novel inhibitor 12/15-lipoxygenase, LOXBlock-1, protected neuronal HT22 cells against oxidative stress. In model transient...
Parkinson's disease (PD) is a progressive neurodegenerative movement disorder characterized by the loss of nigrostriatal dopaminergic neurons. Mounting evidence suggests that Nrf2 promising target for neuroprotective interventions in PD. However, electrophilic chemical properties canonical Nrf2-based drugs cause irreversible alkylation cysteine residues on cellular proteins resulting side effects. Bach1 known transcriptional repressor pathway. We report levels are up-regulated PD postmortem...
Submicromolar zinc inhibits α-ketoglutarate-dependent mitochondrial respiration. This was attributed to inhibition of the α-ketoglutarate dehydrogenase complex (Brown, A. M., Kristal, B. S., Effron, M. Shestopalov, I., Ullucci, P. A., Sheu, K.-F. R., Blass, J. P., and Cooper, L. (2000) <i>J. Biol. Chem.</i> 275, 13441–13447). Lipoamide dehydrogenase, a component two other complexes, catalyzes transfer reducing equivalents from bound dihydrolipoate neighboring dihydrolipoamide acyltransferase...
Indole-3-acetic acid (IAA) is a powerful plant growth regulator. The oxidative decarboxylation of IAA by peroxidases thought to be major degradation reaction involved in controlling the vivo level IAA. Horseradish peroxidase isoenzyme C and an anionic tobacco isolated from transgenic Nicotiana sylvestris have been used experiments vitro designed determine mechanism oxidation. In particular, initial reduction ferric ferrous enzyme, key step previously proposed mechanisms, has investigated...
Impaired glucose metabolism, decreased levels of thiamine and its phosphate esters, reduced activity thiamine-dependent enzymes, such as pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase transketolase occur in Alzheimer's disease (AD). Thiamine deficiency exacerbates amyloid beta (Aβ) deposition, tau hyperphosphorylation oxidative stress. Benfotiamine (BFT) rescued cognitive deficits Aβ burden precursor protein (APP)/PS1 mice. In this study, we examined whether BFT confers...
An amperometric flow system combined with a glucose oxidase-mutarotase reactor was optimized and used to determine aromatic amines phenols using peroxidase-modified graphite electrodes. increase in currents upon injection of the analyzed substrate shown be approximated by Michaelis-Menten type dependence. The detection limit calculated as 3 times noise, sensitivity Imax/K(m)app. Commercially available horseradish peroxidase compared tobacco anionic peanut cationic peroxidases for...
The development of bioelectronic enzyme applications requires the immobilization active proteins onto solid or colloidal substrates such as gold. Coverage gold surface with alkanethiol self-assembled monolayers (SAMs) reduces nonspecific adsorption and also allows incorporation ligands affinity for complementary binding sites on native proteins. We present in this work a strategy covalent glycosylated previously adsorbed through weak, reversible interactions, tailored SAMs. Boronic acids,...