A5020201354- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Renal Transplantation Outcomes and Treatments
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- Pancreatic function and diabetes
- Multiple Myeloma Research and Treatments
- Diabetes Management and Research
- Hepatitis C virus research
- CAR-T cell therapy research
- HIV Research and Treatment
- Mesenchymal stem cell research
- Organ Transplantation Techniques and Outcomes
- Pluripotent Stem Cells Research
- Cytomegalovirus and herpesvirus research
- Parasitic infections in humans and animals
- CRISPR and Genetic Engineering
- Chemokine receptors and signaling
- Parasitic Infections and Diagnostics
- Radiopharmaceutical Chemistry and Applications
- Urticaria and Related Conditions
- Full-Duplex Wireless Communications
Universität Hamburg
2010-2021
University Medical Center Hamburg-Eppendorf
2010-2021
University of Oxford
2011
New York Medical College
2011
University of Freiburg
2011
Paracelsus-Kliniken
2011
Stanford University
2011
Berlin-Brandenburger Centrum für Regenerative Therapien
2011
Leibniz Institute of Virology (LIV)
2009
Martini-Klinik
2007
Hepatocellular carcinoma (HCC) is the fifth most common malignancy worldwide with a poor prognosis and limited therapeutic options. To aid development of novel immunological interventions, we studied breadth, frequency, tumor-infiltration naturally occurring CD8+ T-cell responses targeting several tumor-associated antigens (TAA). We used overlapping peptides spanning entire alpha-fetoprotein (AFP), glypican-3 (GPC-3), melanoma-associated gene-A1 (MAGE-A1) New York-esophageal squamous cell...
We here present an immunologic head-to-head comparison between human umbilical cord lining mesenchymal stem cells (clMSCs) and adult bone marrow MSCs (bmMSCs) from patients >65 years of age. clMSCs had significantly lower HLA class I expression, higher production tolerogenic TGF-β IL-10, showed faster proliferation. In vitro activation allogeneic lymphocytes xenogeneic in vivo immune was stronger with bmMSCs, whereas recognition weaker. Thus, bmMSCs were more quickly rejected immunocompetent...
The HLA-DR15 haplotype is the strongest genetic risk factor for multiple sclerosis (MS), but our understanding of how it contributes to MS limited. Because autoreactive CD4+ T cells and B as antigen-presenting are involved in pathogenesis, we characterized immunopeptidomes two allomorphs DR2a DR2b human primary monocytes, thymus, brain tissue. Self-peptides from HLA-DR molecules, particularly themselves, abundant on thymic cells. Furthermore, identified cell clones that can cross-react with...
Mesenchymal stromal cells (MSCs) have been isolated from many tissues, including gestational tissue. To date, a study comparing the properties and suitability of these in cell-based therapies is lacking. In this study, we compared phenotype, proliferation rate, migration, immunogenicity, immunomodulatory capabilities human MSCs derived umbilical cord lining (CL-MSCs), blood (CB-MSCs), placenta (P-MSCs), Wharton's jelly (WJ-MSCs). Differences were noted differentiation, proliferation, with...
Summary We compared antithymocyte globulin (ATG) with alemtuzumab in 73 patients multiple myeloma, who underwent reduced conditioning melphalan/fludarabine, followed by allogeneic stem cell transplantation from human leucocyte antigen‐matched or ‐mismatched unrelated donors. The ATG group had more prior high‐dose chemotherapies ( P < 0·001), while bone marrow was used as the source 0·001). Alemtuzumab resulted faster engraftment of leucocytes = 0·03) and platelets 0·02) a lower incidence...
The effect of natural killer (NK) cell alloreactivity on outcome unrelated stem transplantation (SCT) remains controversial. Killer immunoglobulin-like receptors (KIRs) recognize human leukocyte antigen C and B epitopes target cells, thereby regulating NK activity. KIR genes are polymorphic two broad haplotypes exist: KIR-haplotype A mainly encode for inhibitory only one activating (KIR2DS4), whereas the group haplotype encodes more KIRs (KIR2DS1, KIR2DS2, KIR2DS3, KIR2DS5 und KIR3DS1).The...
Recent studies indicate that murine Tregs highly express the ENTDP1, as well 5'-NT and thereby, suppress Teff function by extracellular adenosine production. Furthermore, CD73 seems to play a role costimulatory molecule for T cell differentiation. In this study, we analyzed expression of on peripheral lymph nodal Teffs in cohort 95 HIV patients at different stages disease, including LTNP ECs. contrast Tregs, was only expressed small minority (∼10%) Tregs. contrast, see high CD8(+) cells....
The autoimmune phenomena associated with destruction of the beta cell in pancreatic islets and development type 1 (insulin-dependent) diabetes mellitus (IDDM) include circulating islet antibodies. We have immortalized peripheral blood lymphocytes from prediabetic individuals patients newly diagnosed IDDM by Epstein-Barr virus transformation. IgG-positive cells were selected anti-human IgG-coupled magnetic beads expanded culture. Supernatants screened for cytoplasmic antibodies using...
Very little is known about the number and function of immunosuppressive CD4(+)CD25(+)FOXP3(+) T regulatory cells (Treg) in human bone marrow it unclear whether marrow-residing Treg are capable regenerating following allogeneic stem cell transplantation. This particularly surprising since represents a major priming site for T-cell responses play important roles prevention T-cell-mediated graft-versus-host disease promoting tumor escape from T-cell-dependent immunosurveillance.Applying flow...
Failure of the adaptive immune response to control infection with hepatitis C virus (HCV) can result from mutational escape in targeted T-cell epitopes. Recent studies suggest that pressure is an important factor evolution nonstructural proteins HCV. The aim this study was characterize forces contribute viral HLA-A*01-restricted epitope HCV NS3. This represents a potentially attractive target for vaccination strategies since it conserved across all genotypes. In our cohort subjects chronic...
The HLA-DR15 haplotype confers the largest part of genetic risk to develop multiple sclerosis, a prototypic CD4+ T cell-mediated autoimmune disease. mechanisms how certain HLA-class II molecules functionally contribute diseases are still poorly understood, but probably involve shaping an autoimmune-prone cell repertoire during central tolerance in thymus and subsequently maintaining or even expanding it peripheral immune system. Self-peptides that presented by disease-associated most likely...
Abstract: Evidence in animal intermediate hosts that susceptibility to larval infection with Echinococcus multilocularis is restricted individual host factors prompted us investigate the markers humans. Because antigens of extracellular parasite E are possibly presented by MHC molecules a way, we speculated polymorphism may influence resistance towards and course disease. We studied HLA‐A, ‐B, ‐DRB1, ‐DQB1 ‐DPB1 151 patients alveolar echinococcosis. Patients an observation period more than 2...
Human embryonic stem cells (hESCs) can serve as a universal cell source for emerging or tissue replacement strategies, but immune rejection of hESC derivatives remains an unsolved problem. Here, we sought to describe the mechanisms naïve hESCs and upon HLA class I (HLA I) knockdown (hESC(KD)). were I-positive negative II co-stimulatory molecules. Transplantation into immunocompetent Balb/c mice induced substantial T helper 1 2 (Th1 Th2) responses with rapid death, survived in immunodeficient...
Although human embryonic stem cells (hESC) have enormous potential for cell replacement therapy of heart failure, immune rejection hESC derivatives inevitably would occur after transplantation. We therefore aimed to generate a hypoantigeneic line with improved survival characteristics.Using various in vivo, nonischemic, hindlimb xenotransplant models (immunocompetent and defined immunodefective mouse strains) as well vitro T-cell natural killer (NK)-cell assays, we revealed central role T...
T-cell–mediated loss of pancreatic β-cells is the crucial event in development type 1 diabetes. The phenotypic characteristics disease-associated T-cells diabetes have not yet been defined. negative results from two intervention trials (the Diabetes Prevention Trial–Type and European Nicotinamide Intervention Trial) illustrate need for technologies to specifically monitor ongoing autoimmune reactions. We used fluorescence-activated cell sorter analysis study surface marker expression on...
Between August 1996 and December 2004, 369 patients with a median age of 41 years (range: 1-68 years) received stem cell transplantation (SCT) from unrelated donors after an antithymocyte-globulin (ATG)-containing conditioning regimen. In 268 patients, complete molecular typing (4-digit) HLA-A, -B, -C, -DRB1, -DQB1 was available: 110 were completely matched for 10 alleles, 91 had 1 allele-mismatch (9/10), 67 mismatched 2-4 alleles (6-8/10). The incidence grade II-IV acute graft-versus-host...
Virus-specific CD4(+) T cells play a central role in control of viral pathogens including JC polyoma virus (JCV) infection. JCV is ubiquitous small DNA that leads to persistent infection humans with no clinical consequences. However, under circumstances immunocompromise, it able cause an opportunistic and often fatal the brain called progressive multifocal leukoencephalopathy (PML). PML has emerged as serious adverse event multiple sclerosis patients treated anti-VLA-4 mAb natalizumab, which...
To characterize the clinical and immunological features of HLA-typed youth with pediatric onset type 2 diabetes mellitus (T2DM).One hundred seven patients clinically diagnosed T2DM (aged ≤20 yr at diagnosis) were examined. DNA serum, obtained after a median duration 2.2 (Q1-Q3: 0.8-4.6) yr, used for centralized HLA-typing autoantibody (GADA, IA-2A, ZnT8A) measurements.64.6% female age diagnosis was 13.8 11.6-15.4) yr. Patients obese [median body mass index-standard deviation score (BMI-SDS):...