A5082568756- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Blood transfusion and management
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Platelet Disorders and Treatments
- Ethics and Legal Issues in Pediatric Healthcare
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
University of Iceland
2013-2020
National University Hospital of Iceland
2013-2020
Background Mesenchymal stem cells are promising candidates in regenerative cell therapy. Conventional culture methods involve the use of animal substances, specifically fetal bovine serum as growth supplement. Since animal-derived products is undesirable for human applications, platelet lysates produced from platelets an attractive alternative. This especially true if already approved transfusion units at blood banks can be utilized. The purpose this study was to produce expired,...
Platelet lysates have been reported as suitable cell culture supplement for cultures of mesenchymal stromal cells (MSCs). The demand safe and animal-free MSCs is linked to the potential application in clinics. While use platelet offers an alternative animal serum MSC cultures, obtaining supplies fresh concentrates lysate production challenging raises concerns due already existing shortage donors. We previously demonstrated that expired may represent a good source platelets without competing...
Background Clinical application of mesenchymal stromal cells (MSCs) usually requires an in vitro expansion step to reach clinically relevant numbers. In cell necessitates supplementation basal mammalian culture medium with growth factors. To avoid using supplements containing animal substances, human platelet lysates (hPL) produced from expired and pathogen inactivated concentrates can be used place fetal bovine serum. However, globally, most transfusion units are currently not inactivated....
Human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells are mesenchymal-like cells, derived from human without the aid of feeder cells. They have been suggested as a potential alternative to stromal (MSCs) in regenerative medicine due their proliferation and differentiation characteristics. Cells cell products intended for humans should be derived, expanded differentiated using conditions free animal-derived minimize risk animal-transmitted disease immune reactions foreign...
Abstract Background Clinical application of mesenchymal stromal cells (MSCs) usually requires an in vitro expansion step to reach clinically relevant numbers. In cell necessitates supplementation basal mammalian culture medium with growth factors. To avoid using supplements containing animal substances, human platelet lysates (hPL) produced from expired and pathogen inactivated concentrates can be used place fetal bovine serum. Due lack experience global diversity bacterial detection...