A5035810265- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Influenza Virus Research Studies
- CRISPR and Genetic Engineering
- Synthesis and Biological Evaluation
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Autism Spectrum Disorder Research
- Insect symbiosis and bacterial influences
- Transgenic Plants and Applications
- Genetic Syndromes and Imprinting
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Signaling Pathways in Disease
- Genetic Associations and Epidemiology
- Folate and B Vitamins Research
Oregon Health & Science University
2013-2025
University of Edinburgh
2024
University of California, Los Angeles
2024
Stanford University
2024
Cold Spring Harbor Laboratory
2024
Stanford Cancer Institute
2024
Charles Darwin University
2012
Royal Devon and Exeter Hospital
2010
Scottish National Blood Transfusion Service
1998-2007
University of Aberdeen
2004-2005
Evidence of genetic association between the NRG1 (Neuregulin-1) gene and schizophrenia is now well-documented. Furthermore, several recent reports suggest single-nucleotide polymorphisms (SNPs) in ERBB4, one receptors for Neuregulin-1. In this study, we have extended previously published associations by investigating involvement all eight genes from ERBB NRG families with schizophrenia.
Studies of Leishmania donovani have shown that both ornithine decarboxylase and spermidine synthase, two enzymes the polyamine biosynthetic pathway, are critical for promastigote proliferation required maximum infection in mice. However, importance arginase (ARG), first enzyme pathway Leishmania, has not been analyzed L. To test ARG function intact parasites, we generated Δarg null mutants evaluated their ability to proliferate vitro trigger infections The knockout was incapable growth...
The ability of Leishmania to survive in their insect or mammalian host is dependent upon an sense and adapt changes the microenvironment. However, little known about molecular mechanisms underlying parasite response environmental changes, such as nutrient availability. To elucidate stress pathways donovani, we have used purine starvation paradigm. salvage purines from milieu obligatory for replication; nevertheless, purine-starved parasites can persist culture without supplementary over...
Kinetoplastid parasites cause diseases that threaten human and animal health. To survive transitions between vertebrate hosts insect vectors, these rely on precise regulation of gene expression to adapt environmental changes. Since in kinetoplastids is primarily post-transcriptional, developing efficient genetic tools for modifying genes at their endogenous loci while preserving regulatory mRNA elements crucial studying complex biology. We present a CRISPR/Cas9-based tagging system preserves...
A cis-acting methylation center that signals<i>de novo</i> DNA is located upstream of the mouse<i>Aprt</i> gene. In current study, two approaches were taken to determine if tandem B1 repetitive elements found at 3′ end contribute signal. First, bisulfite genomic sequencing demonstrated CpG sites within methylated relative levels 43% in embryonal stem cells deficient for maintenance methyltransferase when compared with wild type cells. Second, ability signal <i>de upon stable transfection...
The promoter region of the mouse adenine phosphoribosyltransferase (aprt) gene contains one nonconsensus Sp1 binding site at its 5′ end followed by three consensus sites. two 3′-most sites are sufficient for maximal expression aprt, suggesting that non-consensus and redundant. However, 3′ not to block epigenetic inactivation, which led hypothesis redundant and/or required inactivation events. To test this hypothesis, constructs were made in mutated alone or tandem, then each construct was...
Mutations within the polyamine biosynthetic pathway of Leishmania donovani, etiological agent visceral leishmaniasis, confer auxotrophy to insect vector or promastigote form parasite. However, whether infectious amastigote parasite requires an intact has remained open question. To address this issue, conditionally lethal Deltaodc mutants lacking ornithine decarboxylase (ODC), rate-limiting enzyme in biosynthesis, were created by double targeted gene replacement a virulent strain L. donovani....
Starvation of Leishmania donovani parasites for purines leads to a rapid amplification in purine nucleobase and nucleoside transport. Studies with transport-deficient L. indicate that this phenomenon is mediated by the transporters LdNT1 LdNT2, as well transporter LdNT3. The escalation transport cannot be ascribed an increase either or LdNT2 mRNA. However, Western analyses on expressing epitope-tagged revealed marked upregulation protein at cell surface. Kinetic investigations activities...
To investigate the attitudes of healthcare professionals towards individuals with traumatic brain injury (TBI) and their relationship to intended behaviour.An independent groups design utilized four variables; aetiology, group, blame gender explore survivors injury. The dependent variables were measured using Prejudicial Evaluation Social Interaction Scale (PESIS) Helping Behaviour (HBS).A hypothetical vignette based methodology was used. Four hundred sixty participants (131 trainee nurses,...
The final two steps of de novo uridine 5'-monophosphate (UMP) biosynthesis are catalyzed by orotate phosphoribosyltransferase (OPRT) and orotidine decarboxylase (OMPDC). In most prokaryotes simple eukaryotes these enzymes encoded separate genes, whereas in mammals they expressed as a bifunctional gene product called UMP synthase (UMPS), with OPRT at the N terminus OMPDC C terminus. Leishmania some closely related organisms also express enzyme for steps, but domain order is reversed relative...
In Leishmania mexicana parasites, a unique glucose transporter, LmxGT1, is selectively targeted to the flagellar membrane, suggesting possible sensory role that often associated with ciliary membrane proteins. Expression of LmxGT1 down-regulated ~20-fold by increasing cell density but up-regulated ~50-fold depleting from medium, and permease strongly when flagellated insect-stage promastigotes invade mammalian macrophages transform into intracellular amastigotes. Regulation expression during...
Leishmania donovani infection of macrophages results in quantitative and qualitative changes the protein profile extracellular vesicles (EVs) released by infected host cells. We confirmed mass spectrometry orthogonally performing Western blots for several -infected macrophage-enriched EVs (LieEVs) molecules. Several cell proteins LieEVs have been implicated promoting vascular other systems. also identified 59 parasite-derived LieEVs, including a putative L. homolog mammalian vasohibins...
ASARs are long noncoding RNA genes that control replication timing of entire human chromosomes in cis. The three known ASAR located on 6 and 15, essential for chromosome integrity. To identify all we utilize a set distinctive characteristics allow the identification hundreds autosomal loci with epigenetically controlled, allele-restricted behavior expression coding genes, is distinct from genomic imprinting. Disruption at five tested result chromosome-wide delayed chromosomal instability,...
Protozoan parasites of the Leishmania genus express metabolic machinery to synthesize pyrimidine nucleotides via both de novo and salvage pathways. To evaluate relative contributions biosynthesis homeostasis in life cycle stages donovani, individual mutant lines deficient either carbamoyl phosphate synthetase (CPS), first enzyme biosynthesis, uracil phosphoribosyltransferase (UPRT), a enzyme, or CPS UPRT were constructed. The Δcps lesion conferred auxotrophy growth requirement for medium...
We investigated the RGS4 as a susceptibility gene for schizophrenia in Chinese Han (184 trios and 138 sibling pairs, total of 322 families) Scottish (580 cases 620 controls) populations using both family trio case‐control design. Both samples had statistical power greater than 70% to detect heterozygote genotype relative risk >1.2 frequent RGS4‐risk alleles. genotyped four single nucleotide polymorphisms (SNPs) which have previously been associated with either individually or part...