A5057005039- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Uterine Myomas and Treatments
- Mechanisms of cancer metastasis
- Endometriosis Research and Treatment
- FOXO transcription factor regulation
- Cancer Mechanisms and Therapy
- Cancer-related molecular mechanisms research
- Chromatin Remodeling and Cancer
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Renal cell carcinoma treatment
- Estrogen and related hormone effects
- Angiogenesis and VEGF in Cancer
- Drug Transport and Resistance Mechanisms
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Reproductive Biology and Fertility
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Trace Elements in Health
Nagoya City University
2024-2025
Shimane University
2014-2023
Kansai Medical University
2017-2022
University of Shimane
2011-2021
Tamagawa University
2018
Shimane University Hospital
2018
Kawasaki Heavy Industries (Japan)
2013-2017
Niigata University of Health and Welfare
2016
Johns Hopkins University
2005-2012
Mount Sinai Hospital
2012
Ovarian clear cell carcinoma (OCCC) is an aggressive human cancer that generally resistant to therapy. To explore the genetic origin of OCCC, we determined exomic sequences eight tumors after immunoaffinity purification cells. Through comparative analyses normal cells from same patients, identified four genes were mutated in at least two tumors. PIK3CA, which encodes a subunit phosphatidylinositol-3 kinase, and KRAS, well-known oncoprotein, had previously been implicated OCCC. The other...
BackgroundUterine cancer is the fourth most common malignancy in women, and uterine serous carcinoma aggressive subtype. However, molecular pathogenesis of largely unknown. We analyzed genomes samples to better understand genetic characteristics this cancer.
Abstract Gene amplification is one of the common mechanisms that activate oncogenes. In this study, we used single nucleotide polymorphism array to analyze genome-wide DNA copy number alterations in 31 high-grade ovarian serous carcinomas, most lethal gynecologic neoplastic disease women. We identified an amplicon at 19p13.12 6 (19.5%) carcinomas. This was validated by digital karyotyping, quantitative real-time PCR, and dual-color fluorescence situ hybridization (FISH) analysis....
It has been well established that ovarian low-grade and high-grade serous carcinomas are fundamentally different types of tumours. While the molecular genetic features now known, pathogenesis carcinomas, apart from recognition frequent somatic mutations involving KRAS BRAF, is largely unknown. In order to comprehensively analyse in we applied exome sequencing DNA eight samples affinity-purified, low-grade, carcinomas. A remarkably small number were identified seven these tumours: a total 70...
Sequence mutations and gene amplifications lead to activation of the PIK3CA-AKT2 signaling pathway have been reported in several types neoplasms including ovarian cancer. Analysis such genetic alterations, however, is usually complicated by contamination normal cell DNA, artifacts associated with formalin-fixed tissues sensitivity techniques employed. In this study, we analyzed sequence PIK3CA AKT2 genes using purified tumor cells that were isolated from high-grade serous carcinomas...
This study examined the clinical significance of CCNE1 (Cyclin E1) amplification and assessed whether is a potential therapeutic target in ovarian cancer.CCNE1 expression cancer was by immunohistochemistry, fluorescence situ hybridization data collected retrospective chart review. gene knockdown using silencing RNA transfection system were used to asses function tissue samples cancer.Gene identified 18 (20.4%) 88 carcinomas. copy number significantly correlated with protein (r = 0.522, P <...
// Hitomi Yamashita 1 , Kentaro Nakayama Masako Ishikawa Kohei Nakamura Tomoka Ishibashi Kaori Sanuki Ruriko Ono Hiroki Sasamori Toshiko Minamoto Kouji Iida Razia Sultana Noriyoshi 2 and Satoru Kyo Department of Obstetrics Gynecology, Shimane University School Medicine, Izumo, Japan Organ Pathology, Correspondence to: Nakayama, email: Keywords : microsatellite instability; endometrial cancer; immune checkpoint inhibitor; immunohistochemistry; mismatch repair protein; Immunology Received...
Intrinsic or acquired resistance to chemotherapy is the major obstacle overcome in treatment of patients with solid carcinoma. Cisplatin one most effective chemotherapeutic agents for treating ovarian Recently, copper-transporting P-type adenosine triphosphatase (ATP7B) has been demonstrated as genes responsible cisplatin vitro. We hypothesized that expression ATP7B gene increases carcinoma and a priori knowledge its important choice therapy. The aim our study was assess role compare those...
A genomewide technology, digital karyotyping, was used to identify subchromosomal alterations in ovarian cancer. Amplification at 11q13.5 found three of seven carcinomas, and amplicon mapping delineated a 1.8-Mb core amplification that contained 13 genes. FISH analysis demonstrated this region 13.2% high-grade carcinomas but not any low-grade or benign tumors. Combined genetic transcriptome analyses showed Rsf-1 (HBXAPalpha) the only gene consistent overexpression all tumors harboring...
Ovarian serous carcinoma is the most common and lethal type of ovarian cancer its molecular etiology remains poorly understood. As an ongoing effort to elucidate pathogenesis carcinomas, we assessed DNA copy number changes in 33 high-grade carcinomas 10 low-grade tumors by using a genome-wide technique, single nucleotide polymorphism array, performed on affinity-purified tumor cells from fresh surgical specimens. Compared tumors, showed widespread changes. The frequent alterations were loci...
Recent studies have suggested an oncogenic role of the BTB/POZ-domain genes in hematopoietic malignancy. The aim this study is to identify and characterize development human epithelial cancers, i.e., carcinomas. In study, we focused on ovarian carcinoma analyzed gene expression levels using serial analysis (SAGE) data all 130 deduced BTB/POZ genes. Our reveals that NAC-1 significantly overexpressed serous carcinomas several other types Immunohistochemistry demonstrate localized discrete...
This study examined the status of KRAS and BRAF mutations, in relation to extracellular signal-regulated protein kinase (ERK) activation 58 ovarian carcinomas clarify clinicopathological prognostic significance KRAS/BRAF mutations. Somatic mutations either or were identified 12 (20.6%) out carcinomas. The frequency conventional serous high-grade (4.0% : 1/25) was significantly lower than that other histological type (32.3% 10/31). Phosphorylated ERK1/2 (p-ERK1/2) expression 18 (38.2%) 45...
Advanced ovarian clear cell carcinoma (CCC) is one of the most aggressive malignancies, in part because it tends to be resistant platinum-based chemotherapy. At present, little known about molecular genetic alterations CCCs except that there are frequent activating mutations PIK3CA. The purpose this study comprehensively define genomic changes CCC based on DNA copy number alterations.We performed 250K high-density single nucleotide polymorphism array analysis 12 affinity-purified and 10...
A major obstacle in the treatment of ovarian carcinoma is intrinsic/acquired resistance to cisplatin-based chemotherapy. Cu-transporting ATPase (ATP7B) has been reported be associated with cisplatin vitro. However, clinical significance this transporter not previously addressed. Our goal was investigate ATP7B expression and whether its correlates prognosis reduced responsiveness treatment.We retrospectively examined p53 primary association chemotherapeutic effect. Tissues were surgically...
The expression levels of mRNA for multidrug resistance 1 (MDR1) gene, protein (MRP1), lung resistance-related (LRP) and breast cancer (BCRP), which confer in vitro, were examined 43 untreated carcinoma patients, whom 38 subsequently received doxorubicin-based chemotherapy after surgery, order to elucidate the roles these genes drug vivo. determined using a semi-quantitative reverse-transcription polymerase chain reaction method tissues including at least 80% cells. level BCRP gene was low...
The aim of this study was to investigate the patterns epidermal growth factor receptor (EGFR) overexpression, EGFR gene amplification, and presence activating mutations in tyrosine kinase domain squamous cell carcinomas adenocarcinomas/adenosquamous uterine cervix.The expression, mutation cervical were assessed by immunohistochemistry, fluorescence situ hybridisation, PCR-SSCP, respectively, correlated with clinical data collected a retrospective chart review. A functional assessment...
This study examined the clinical significance of Notch3 expression and assessed its usefulness as a potential therapeutic target in chemoresistant ovarian cancer. was with immunohistochemical examination, variables were collected retrospective chart review. siRNA or γ-secretase inhibitor used to assess function cancer cell lines. overexpression correlated shorter progression-free/overall survival patients advanced stage (stage III, IV) carcinoma treated platinum taxane. Three 5 showed...
The clinicopathological significance of amplification was investigated the gene encoding cyclin E (CCNE1) and we assessed whether CCNE1 a potential target in endometrioid endometrial carcinomas. or F-box WD repeat domain-containing 7 (FBXW7) expression carcinoma by immunohistochemistry fluorescence situ hybridization. knockdown small interfering RNA (siRNA) used to assess function. results showed that present 9 (8.3%) 108 correlated with high histological grade (Grade 3; p=0.0087)...
High-grade serous ovarian carcinoma (HGSOC) typically remains undiagnosed until advanced stages when peritoneal dissemination has already occurred. Here, we sought to identify HGSOC-specific alterations in DNA methylation and assess their potential provide sensitive specific detection of HGSOC at its earliest stages.MethylationEPIC genome-wide analysis was performed on a discovery cohort comprising 23 HGSOC, 37 non-HGSOC malignant, 36 histologically unremarkable gynecologic tissue samples....