A5044487894- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Chemical Synthesis and Analysis
- Protein purification and stability
- Click Chemistry and Applications
- Glycosylation and Glycoproteins Research
- 3D Printing in Biomedical Research
- Head and Neck Cancer Studies
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Management of metastatic bone disease
- Lung Cancer Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Salivary Gland Tumors Diagnosis and Treatment
- Cellular Mechanics and Interactions
Jichi Medical University
2021-2025
Jichi Medical University Saitama Medical Center
2021-2025
National Institute of Biomedical Innovation, Health and Nutrition
2021-2024
The University of Tokyo
2014-2024
Kouseiren Takaoka Hospital
2021
Musashino University
2020
Taiho Pharmaceutical (Japan)
2014
Matsumoto University
2014
Kyoto University Hospital
2013
Albert Einstein College of Medicine
2009-2012
1. In Mg(2+)-free external solution, rat cortical neurons in cultured networks entered a stable firing mode, consisting of regular bursts action potentials superimposed on long-lasting depolarizations. The average separation between varied from culture to culture, but was usually 5 and 20 s. distribution burst intervals followed Gaussian or normal distribution, with standard deviation typically 10% the period. 2. A gradually depolarizing pacemaker potential never observed bursts, threshold...
Abstract In cultures of rat cortical neurons, we found that stimulation tyrosine receptor kinase B (TrkB) with brain‐derived neurotrophic factor (BDNF) induced a biphasic expression BDNF exon IV–IX mRNA, which became obvious 1–3 h (primary induction) and 24–72 (delayed after the stimulation, characterized delayed induction in relation to mRNA activity‐regulated cytoskeleton‐associated protein (Arc). Withdrawal from medium for 3 allowed induction, was caused at transcriptional level dependent...
Abstract Previous experiments using enzyme inhibitors and RNA interference in cell lysates cultured cells have suggested that tripeptidyl peptidase II (TPPII) plays a role creating destroying MHC class I-presented peptides. However, its precise contribution to these processes has been controversial. To elucidate the importance of TPPII I Ag presentation, we analyzed TPPII-deficient gene-trapped mice lines from animals. In mice, expression level was reduced by >90% compared with...
Introduction: Although cone-beam computed tomography (CBCT) is essential for tumor positioning in image-guided radiotherapy, its diagnostic application limited by low image quality. This report highlights a case where CBCT unexpectedly detected osteoblastic bone metastasis during radiotherapy prostate cancer. Case Presentation: A 65-year-old man with recurrent cancer underwent postoperative intensity-modulated (IMRT) of 66 Gy 33 fractions. Two months after treatment, an increase...
Although cytokine receptors regulate many cellular functions, contribution of receptor's domains and their conformation to signal transduction remains unclear. In this study, we designed a series chimeric erythropoietin receptor (EpoR) variants encoding haemagglutinin epitope-tagged anti-fluorescein single-chain Fv different combinations extracellular D1/D2 domain(s) EpoR as the domain allow be activated by multiple ligands. Furthermore, one four Ala residues were inserted at intracellular...
Detection of protein–protein interactions (PPIs) is important for understanding numerous processes in mammalian cells; however, existing PPI detection methods often give significant background signals. Here, we propose a novel PPI-detection method based on kinase-mediated growth induction cells. In this method, target proteins are fused to the intracellular domain c-kit (c-kit ICD) and expressed interleukin-3-dependent The induces dimerization activation ICDs, which leads cell absence...
We previously demonstrated that cultured human fibroblasts internalize iron via transferrin-independent uptake (Tf-IU), redox, and receptor-mediated endocytosis systems [Oshiro, S., Nakajima, H., Markello, T., Krasnewich, D., Bernardini, I., Gahl, W.A. (1993) J. Biol. Chem. 268, 21586 21591]. Of these transport systems, the Tf-IU system is involved in accumulation of transition metals various mammalian cells. It also known experimental animals fed aluminum (Al), Al at micromolar level...
Although receptor tyrosine kinases (RTKs) play a pivotal role in the development and maintaining homeostasis of body, overexpression or mutation RTKs often induces tumorigenesis metastasis. To mimic function RTKs, we developed two fusion receptors consisting anti-fluorescein antibody single-chain Fv, extracellular D2 domain erythropoietin transmembrane/intracellular domains epidermal growth factor c-fms based on previously constructed antibody/cytokine chimeras. The expression these chimeric...
Since many cell functions are regulated by members of the cytokine receptor superfamily, artificial mimicry system would be attractive for cellular engineering. We previously showed that an antibody/cytokine chimera can transduce a growth signal in response to non-natural ligands, such as fluorescein-conjugated BSA. However, considerable background proliferation was observed without antigen. Therefore, we redesigned chimeric constructs with different combinations domains containing...
Cell patterning on photo-responsive materials are a promising tool for preparing unique single-cell arrays. However, most conventional arrays such smart can be applied only to adherent cells and limit cellular functions as extension migration within the patterned adhesive surfaces. In this study, versatile single cell array that works with both non-adherent was constructed using photo-cleavable polyethylene glycol (PEG)-lipid/collagen surface. On array, behaved similar their native without...
While antibiotic selection has been routinely used for the of genetically modified cells, administration cytotoxic drugs often leads to deleterious effects not only inert cells but also transfected or transduced ones. In this study, we propose an Antigen-MEdiated Genetically cell Amplification (AMEGA) system employing antibody/receptor chimeras without selection. Based on a rational design where extracellular domains dimeric erythropoietin receptor (EpoR) gp130 were substituted with...