The surface engagement of high affinity immunoglobulin E receptor (Fc epsilon RI) rat basophilic leukemia 2H3 (RBL-2H3) cells induced histamine secretion and leukotriene release following activation the tyrosine kinase Lyn together with phosphatidylinositol 3-kinase (PI3-kinase). Wortmannin inhibited activity partially purified PI3-kinase from calf thymus, as well in anti-PI3-kinase p85 immunoprecipitates RBL-2H3 cells, at a concentration low 1.0 nM IC50 values 3.0 nM, but did not inhibit...

Theoretical study is presented on the wetting behaviors of water droplets over a lotus leaf. Experimental results are interpreted to clarify trade-offs among potential energy change, local pinning energy, and adhesion energy. The theoretical parameters, calculated from experimental results, used qualitatively explain relations surface fractal dimension, morphology, dynamic behaviors. leaf, which shows superhydrophobic effect, was dipped in ethanol remove plant waxes. As result, effect lost....

Chemically skinned fibers from guinea pig taenia caecum were prepared by saponin treatment to study the smooth muscle contractile system in a state as close living posible. The showed tension development with an increase of Ca2+ solution, threshold occurring 5 X 10(-7) M Ca2+. maximal induced 10(-4) was large and rapid potassium-induced contracture intact fibers. slope pCa curve less steep than that skeletal shifted direction lower MgATP. presence greater 1 mM Mg2+ required for Ca2+-induced...

Tetrodotoxin, at concentrations up to 5 x 10(-6) gram per milliliter, has no effect on the spontaneous discharge in smooth muscle of taenia coli. However, is abolished by Mn(++) a concentration 0.5 millimole liter. The contraction induced immersing isotonic KCl solution also suppressed presence Mn(++). Because specific suppressor spike Ca(++) and tetrodotoxin an inhibitor Na(+), we suggest that charge carrier production potential entry intervening through membrane acts as trigger for muscle.

We examined the potential role of myosin and actin in release human immunodeficiency virus type 1 (HIV-1) from infected cells. Wortmannin (100 nM to 5 microM), an effective inhibitor light chain kinase, blocked HIV-1 T-lymphoblastoid monocytoid cells a concentration-dependent manner. Cytochalasin D, reagent that disrupts equilibrium between monomeric polymeric actin, also partially inhibited At budding stage, protein were detected same areas on plasma membrane by using dual-label...

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTAdsorption of Disk-Shaped Janus Beads at Liquid−Liquid InterfacesYoshimune Nonomura, Shigeyuki Komura, and Kaoru TsujiiView Author Information Tokyo Research Laboratories, Kao Corporation, 2-1-3 Bunka, Sumida-ku, 131-8501, Japan, Department Chemistry, Faculty Science, Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, 192-0397, Nanotechnology Center, Institute for Electronic Hokkaido N21, W10 Kita-ku, Sapporo 001-0021, Japan Cite this: Langmuir...

Double roughness structure, the origin of lotus effect natural leaf, was successfully reproduced on a diarylethene microcrystalline surface. Static superwater-repellency and dynamic water-drop-bouncing were observed surface, in manner leaves. structure essential for water-drop-bouncing. This ability not single surface showing same derivative. The double reversibly controlled by alternating irradiation with UV visible light.

We have isolated two cDNA clones for myosin alkali light chain (MLC) mRNA from respective libraries of chick gizzard and fibroblast cells by cross-hybridization to the previously skeletal muscle MLC. Sequence analysis cloned cDNAs revealed that both them are homologous but distinct sequence used as probe so they may be classified into members MLC family, identical with each other in 3' 5' untranslated well coding a notable exception 39-nucleotide insertion cDNA, 26 nucleotides which encoding...

Abstract The effective resolution of human platelet cytosolic phosphoinositide-phospholipase C (PLC) revealed five distinct activity peaks by Q-Sepharose and heparin-Sepharose column chromatographies when assayed using phosphatidylinositol (PI) 4,5-bisphosphate (PIP2). results Western blotting analysis with various antibodies against PLC isozymes showed that peak-Ia (PLC-delta type), peak-Ib (PLC-gamma 1 peak-IIc (PLC-beta type) two unidentified (PLC-IIa PLC-IIb) were present in cytosol. A...

Effects of K-252a, (8R*, 9S*, 11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8, 11-epoxy-1H,8H,11H-2,7b,11a-triazadibenzo[a,g]cycloocta [cde]trinden-1-one, purified from the culture broth Nocardiopsis sp., on activity myosin light chain kinase were investigated. 1) K-252a (1 x 10(-5) M) affected three characteristic properties chicken gizzard myosin-B, natural actomyosin, to a similar degree: Ca2+-dependent ATPase, superprecipitation, and phosphorylation chain. 2)...

Ca(2+)-induced exocytosis in chromaffin cells now seems to consist of at least two distinct steps:MgATP-dependent Ca(2+)-dependent priming the secretory apparatus, and MgATP-independent step that triggers (Bittner Holz, 1992). Recently we found a specific inhibitor myosin light chain kinase (MLCK), wortmannin, inhibits catecholamine release from digitonin-permeabilized cells, suggesting an implication MLCK mechanisms (Imaizumi et al., 1992b). To elucidate further mechanism exocytosis,...

The solid particles are adsorbed at interfaces and form self-assembled structures when the have suitable wettability to both liquids. Here, we show theoretically how microstructure on particle surface affects their adsorption properties. physical properties of interface adsorbing a will be described by taking into account roughness due microstructure. changes drastically equilibrium position particle. Therefore, contact angle three-phase line shifts with area, because enhances interfacial...

Some researchers have focused on the adsorption of solid particles at fluid−fluid interfaces and prepared emulsions foams called "Pickering emulsions/foams". However, while several reports exist simple spherical emulsions, few are available formation more complex structures. Here, we show that holes particle surfaces a key factor in establishing variety complexity mesoscale Microbowls, which hollow with their surfaces, form multiple (water-in-oil-in-water oil-in-water-in-oil emulsions) by...

Abstract We developed a friction evaluation system with human finger model. The surface of the model was covered imitation fingerprints. When polypropylene rubbed against model, profile varied sliding velocity and vertical force.

Nonlinear phenomena on the soft material surface are one of most exciting topics chemical physics. However, only a few reports exist friction under accelerated movement, because between two solid surfaces is considered linear phenomenon in many cases. We aim to investigate how nonlinear motion affects surfaces. In present study, we evaluate frictional forces polytetrafluoroethylene (PTFE) resins using an advanced evaluation system. On PTFE surfaces, normalized delay time δ, which lag...

Bacterial flora on the skin surface contains Staphylococcus aureus (S. aureus) and epidermidis epidermidis) which causes rough atopic dermatitis enhances innate immunity, respectively. In this study, minimum inhibitory concentration (MIC) was evaluated for six saturated fatty acids two unsaturated against S. epidermidis. The antimicrobial behavior in liquid medium categorized into three groups. first selective antibacterial activity group comprising myristic acid (C14:0 acid), palmitoleic...

Shittori feel is defined as a texture that moderately moisturized; however, many people experience ‘ shittori feel’ when they touch dry solid material containing little liquid. Here, was evaluated for 12 materials. We found the highest score of achieved by powders. Multiple regression analysis showed complex sense moist and smooth feels. analysed relationship between physical properties moist/smooth feels to show how subjects felt certain simultaneously. The are related surface roughness...

Wortmannin inhibited phosphatidylinositol 3-kinase (P13-kinase) and Fc epsilon RI-mediated histamine secretion in RBL-2H3 cells to a similar degree, with IC50 values of 3 2 nM, respectively. Although P13-kinase is an acknowledged regulator intracellular trafficking secretion, wortmannin has proved be difficult drug use assessing the role because it inhibits another important enzyme, myosin light-chain kinase (MLCK; = 200 nM). In present study we synthesized unique derivative wortmannin,...

The regulation of extracellular Ca2+ entry into fura-2-loaded human platelets was examined following stimulation with thrombin. In the presence external Ca2+, thrombin resulted in a rapid increase, followed by plateau, intracellular concentration ([Ca2+]i). Pretreatment wortmannin, specific inhibitor myosin light chain kinase, suppressed only plateau phase and had no effect on initial increase [Ca2+]i. Ca(2+)-free EGTA buffer, induced transient relatively small [Ca2+]i caused release from...

Previously, we have shown that okadaic acid (OA), isolated from black sponge (Halichondria okadai) causes contraction even in the absence of Ca++ saponin-permealized taenia guinea pig cecum. In present study, mechanism action OA was examined using native actomyosin extracted chicken gizzard smooth muscle. Ca++, (0.1-1 microM) induced superprecipitation and increased Mg++-adenosine triphosphatase activity. The OA-induced enhanced by at a concentration (greater than 0.1 which did not activate...

We investigate the adsorption of a spherical Janus particle to spherically curved liquid-liquid interface. show that equilibrium contact angle is determined by geometry particle, its wettability, and also interfacial curvature. In contrast with homogeneous there preferred curvature (spontaneous curvature) due when satisfies certain conditions.