A5036803257- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Diet and metabolism studies
- Autophagy in Disease and Therapy
- Ginseng Biological Effects and Applications
- Beetle Biology and Toxicology Studies
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Lipid metabolism and disorders
- Pharmacological Effects of Natural Compounds
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Tannin, Tannase and Anticancer Activities
- Chromatography in Natural Products
- Polysaccharides and Plant Cell Walls
- Histone Deacetylase Inhibitors Research
- Peroxisome Proliferator-Activated Receptors
- RNA regulation and disease
- Inflammasome and immune disorders
- Hemiptera Insect Studies
Tianjin University of Traditional Chinese Medicine
2018-2025
University of Kentucky
2009-2018
Friedrich-Alexander-Universität Erlangen-Nürnberg
2004-2010
The YTH (YT521-B homology) domain was identified by sequence comparison and is found in 174 different proteins expressed eukaryotes. It characterized 14 invariant residues within an alpha-helix/beta-sheet structure. Here we show that the a novel RNA binding binds to short, degenerated, single-stranded motif. presence of motif alternative exons necessary for YT521-B directly influence splice site selection vivo. Array analyses demonstrate predominantly regulates vertebrate-specific exons. An...
The loss of HBII-52 and related C/D box small nucleolar RNA (snoRNA) expression units have been implicated as a cause for the Prader–Willi syndrome (PWS). We recently found that snoRNA changes alternative splicing serotonin receptor 2C pre-mRNA, which is different from traditional function in non-mRNA methylation. Using bioinformatic predictions experimental verification, we identified five pre-mRNAs (DPM2, TAF1, RALGPS1, PBRM1 CRHR1) containing exons are regulated by MBII-52, mouse homolog...
The secondary structure of a pre-mRNA influences number processing steps including alternative splicing. Since most splicing regulatory proteins bind to single-stranded RNA, the sequestration RNA into double strands could prevent their binding. Here, we analyzed context experimentally determined enhancer and silencer motifs in natural context. We found that these are significantly more than controls. These findings were validated by transfection experiments, where effect or on exon skipping...
Glycyrrhiza Uralensis Polysaccharide (GCP), as a macromolecular polysaccharide extracted from the Traditional Chinese Medicine (TCM) - Licorice has been proved to inhibit tumor growth in vitro and vivo; however, specific anti-tumor mechanism of GCP needs be further investigated. In this study, we explore angle gut microbiota. Colon carcinoma cells (CT-26) were used set up tumor-bearing mouse model. After 14 days treatment, weights tumors significantly reduced. addition, HE staining tissue...
Almost every protein-coding gene undergoes pre-mRNA splicing, and the majority of these pre-mRNAs are alternatively spliced. Alternative exon usage is regulated by transient formation protein complexes on that typically contain heterogeneous nuclear ribonucleoproteins (hnRNPs). Here we characterize hnRNP G, a member class proteins. We show G expressed in different concentrations various tissues interacts with other splicing regulatory part supraspliceosome, where it regulates alternative...
Metabolomic analysis has been used to characterize the effects and mechanisms of drugs for nonalcoholic fatty liver disease (NAFLD) at metabolic level. Nuciferine is an active component derived from folium nelumbinis demonstrated have beneficial on a high-fat diet (HFD) induced hepatic steatosis model. However, effect altered metabolites nuciferine NAFLD not yet elucidated. In this study, we established rat model using HFD treated with nuciferine. The lipid content levels, pro-inflammatory...
There is increasing evidence showing that inflammation associated with depression in humans. Hesperidin, a natural bioflavonoid, has performed excellent effects on depression. The aim of this research was to investigate the therapeutic effect hesperidin chronic unpredictable mild stress (CUMS)-induced rats. sucrose preference test (SPT), forced swimming (FST) and open field (OFT) were measure depression-related symptoms. enzyme-linked immunosorbent assay (ELISA) used determine concentrations...
The dysbiosis in gut microbiota could affect host metabolism and contribute to the development of nonalcoholic fatty liver disease (NAFLD). Da-Chai-Hu decoction (DCH) has demonstrated protective effects on NAFLD, however, exact mechanisms remain unclear. In this study, we established a NAFLD rat model using high fat diet (HFD) provided treatment with DCH. changes post DCH were then investigated 16S rRNA sequencing. Additionally, serum untargeted metabolomics performed examine metabolic...
Necroptosis is a programmed form of necrosis and compounds inducing necroptosis may contribute to cancer treatment. 20(S)-ginsenoside Rg3 natural compound extracted from ginseng, which exhibited broad-spectrum antitumor activity. In the present study, potential role in prostate cells was evaluated. inhibited proliferation upregulated expression necroptotic proteins such as receptor-interacting serine/threonine-protein kinase 1 (RIPK1), RIPK3, their downstream mixed lineage domain-like...
Valproic acid (VPA) is a commonly used drug to treat epilepsy and bipolar disorders. Known properties of VPA are inhibitions histone deacetylases activation extracellular signal regulated kinases (ERK), which cannot fully explain VPA's clinical features. We found that induces the proteasomal degradation DICER, key protein in generation micro RNAs. Unexpectedly, concentration several RNAs increases after treatment, caused by upregulation their hosting genes prior DICER degradation. The data...
Pi-Dan-Jian-Qing decoction (PDJQ) can been used in the treatment of type 2 diabetes mellitus (T2DM) clinic. However, protective mechanisms PDJQ on T2DM remain unknown. Recent studies have shown that changes gut microbiota could affect host metabolism and contribute to progression T2DM. In this study, we first investigated therapeutic effects rats. 16S rRNA sequencing untargeted metabolomics analyses were investigate action Our results showed improve hyperglycemia, hyperlipidemia, insulin...
Baihu Rensheng decoction (BHRS) can effectively improve insulin resistance (IR) and decrease blood glucose in diabetic patients. However, its specific mechanism of action remains unclear. In this study, a type 2 diabetes mellitus (T2DM) rat model was established using high-fat diet combined with streptozotocin (STZ) injection treated BHRS. Firstly, the therapeutic anti-inflammatory effects BHRS on T2DM were evaluated. Secondly, gut permeability evaluated western blot used to detect changes...
Many studies have found that the dysfunction in gut microbiota and metabolic can promote nonalcoholic fatty liver disease (NAFLD) development. Er-Chen decoction (EC) be used treatment of NAFLD. However, mechanism this hepatoprotection is still unknown. In study, we constructed a rat model with NAFLD fed high-fat chow administered EC treatment. The therapeutic effects on were evaluated by measuring transaminases, blood lipid levels, pathological changes liver. addition, measured inflammatory...
Polygonatum sibiricum polysaccharide (PSP) can improve insulin resistance and inhibit oxidative stress. However, the detailed anti-diabetic mechanism of PSP is still poorly defined.In this study, anti-diabetic, anti-inflammatory anti-oxidative effects were evaluated on a type 2 diabetes mellitus (T2DM) rat model. Furthermore, we investigated changes in gut microbiota serum metabolites T2DM rats after treatment through 16S rRNA sequencing untargeted metabolomics analyses.Our results showed...
YT521-B is a ubiquitously expressed nuclear protein that changes alternative splice site usage in concentration dependent manner. located dynamic compartment, the YT body. We show tyrosine phosphorylated by c-Abl nucleus. The shuttles between nucleus and cytosol, where it can be c-Src or p59(fyn). Tyrosine phosphorylation causes dispersion of from bodies to nucleoplasm. Whereas are soluble non-denaturing buffers, phosphorylated, dispersed form non-soluble. Non-phosphorylated selection IL-4...
To investigate the potential antitumor effects of ginsenoside Rg3 in prostate cancer cells, androgen‑insensitive cell line PC3 was cultured and incubated with vitro. Cell number counts, proliferation assays senescence‑associated β‑galactosidase (SA‑β‑gal) staining were performed to evaluate proliferation. The results demonstrated that led arrest; decreased cells increased positive SA‑β‑gal rate cells. cycle analysis by flow cytometry revealed interfered G1/S transition mechanism involved...
Research Article12 July 2016Open Access Source DataTransparent process Oligonucleotide-induced alternative splicing of serotonin 2C receptor reduces food intake Zhaiyi Zhang Department Molecular and Cellular Biochemistry, University Kentucky, Lexington, KY, USA Search for more papers by this author Manli Shen Paul J Gresch Pharmacology, Vanderbilt University, Nashville, TN, Masoud Ghamari-Langroudi Physiology & Biophysics, Alexander G Rabchevsky Spinal Cord Brain Injury, Center, Ronald B...
Alternative pre-mRNA splicing is a central element of eukaryotic gene expression. Its deregulation can lead to disease, and methods change splice site selection are developed as potential therapies. Spinal muscular atrophy caused by the loss SMN1 (survival motoneuron 1) gene. A therapeutic avenue for spinal treatment promote exon 7 inclusion almost identical SMN2 2) The factor tra2-beta1 promotes this antagonized protein phosphatase (PP) 1. To identify new compounds that inclusion, we...
The serotonin receptor 2C (HTR2C) gene encodes a G protein-coupled that is exclusively expressed in neurons. Here, we report the 5' untranslated region of pre-mRNA as well its hosted miRNAs widely non-neuronal cell lines. Alternative splicing HTR2C regulated by MBII-52. MBII-52 and neighboring MBII-85 cluster are absent people with Prader-Willi syndrome, which likely causes disease. We show increase expression UTR influence miRNAs. data indicate transcriptional unit expressing more complex...
The aim of this study was to elucidate the mechanism nuciferine on alleviating obesity based modulating gut microbiota, ameliorating chronic inflammation, and improving permeability. In study, obese model mice were induced by high-fat diet then randomly divided into group, group; some other same week age fed with normal as group. modeling process, administered intragastrically(ig) for 12 weeks. course both treatment, body weight food intake in each group measured weekly. After Lee's index,...
San-Huang-Yi-Shen capsule (SHYS) has been used in the treatment of diabetic kidney disease (DKD) clinics. However, mechanism SHYS on DKD remains unclear. In this study, we a high-fat diet combined with streptozocin (STZ) injection to establish rat model DKD, and different doses were given by oral gavage determine therapeutic effects DKD. Then, studied PINK1/Parkin-mediated mitophagy activation NLRP3 inflammasome study possible mechanisms Our result showed that could alleviate through...