A5100758340- Muscle Physiology and Disorders
- Epigenetics and DNA Methylation
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Exercise and Physiological Responses
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Erythropoietin and Anemia Treatment
- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- Diet and metabolism studies
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- TGF-β signaling in diseases
- Extracellular vesicles in disease
- Spinal Fractures and Fixation Techniques
- Mitochondrial Function and Pathology
- Cardiomyopathy and Myosin Studies
- GDF15 and Related Biomarkers
Sichuan Academy of Traditional Chinese Medicine
2025
University of Kentucky
2016-2025
National Institute for Communicable Disease Control and Prevention
2025
China Three Gorges University
2024
Tianjin Institute of Industrial Biotechnology
2024
Chinese Academy of Sciences
2024
The First People's Hospital of Changzhou
2024
Second Affiliated Hospital of Nantong University
2024
First Affiliated Hospital of Nanchang University
2009-2024
Nanchang University
2009-2024
H3K27 methylation mediated by the histone methyltransferase complex PRC2 is critical for transcriptional regulation, Polycomb silencing, Drosophila segmentation, mammalian X chromosome inactivation, and cancer. PRC2-mediated can spread along chromatin propagate repressive environment; thus, components that antagonize activity of are important restraining silencing. Here we report in HeLa cells, H3 histones unmethylated at Lys-36 mostly methylated Lys-27, with exception newly synthesized H3....
Polycomb repressive complex 2 (PRC2)-mediated histone H3 lysine 27 (H3K27) methylation is vital for gene silencing, a classic epigenetic phenomenon that maintains transcriptional silencing throughout cell divisions. We report PRC2 activity regulated by the density of its substrate nucleosome arrays. Neighboring nucleosomes activate with fragment their histones (Ala(31) to Arg(42)). also identified mutations on subunit Su(z)12, which impair binding and response activating peptide ability in...
Analysis of skeletal muscle cross sections is an important experimental technique in biology. Many aspects immunohistochemistry and fluorescence microscopy can now be automated, but most image quantification techniques still require extensive human input, slowing progress introducing the possibility user bias. MyoVision a new software package that was developed to overcome these limitations. The improves upon previously reported automatic analyzes images without requiring significant input...
Skeletal muscle is a major contributor to whole-body metabolism as it serves depot for both glucose and amino acids, highly metabolically active tissue. Within skeletal exists an intrinsic molecular clock mechanism that regulates the timing of physiological processes. A key function regulate metabolic processes anticipate time day changes in environmental conditions. The purpose this study was identify genes are expressed circadian manner determine if these regulated downstream by assaying...
Pax7+ satellite cells are required for skeletal muscle fiber growth during post-natal development in mice. Satellite cell-mediated myonuclear accretion also appears to persist into early adulthood. Given the important role of development, we hypothesized that necessity adaptation an imposed hypertrophic stimulus depends on maturational age. Pax7CreER-R26RDTA mice were treated 5 days with vehicle (satellite cell-replete, SC+) or tamoxifen cell-depleted, SC-) at 2 months (young) and 4 (mature)...
The endogenous molecular clock in skeletal muscle is necessary for maintenance of phenotype and function. Loss Bmal1 solely from adult (iMSBmal1(-/-) ) results reductions specific tension, increased oxidative fibre type fibrosis with no change feeding or activity. Disruption the sufficient to induce changes similar those seen knockout mouse (Bmal1(-/-) ), a model advanced ageing. iMSBmal1(-/-) mice develop bone calcification decreased joint collagen, which combination functional altered...
How regular physical activity is able to improve health remains poorly understood. The release of factors from skeletal muscle following exercise has been proposed as a possible mechanism mediating such systemic benefits. We describe wherein muscle, in response hypertrophic stimulus induced by mechanical overload (MOV), released extracellular vesicles (EVs) containing muscle-specific miR-1 that were preferentially taken up epidydimal white adipose tissue (eWAT). In eWAT, promoted adrenergic...
Abstract There are functional benefits to exercise in muscle, even when performed late life, but the contributions of epigenetic factors late‐life adaptation poorly defined. Using reduced representation bisulfite sequencing (RRBS), ribosomal DNA (rDNA) and mitochondrial‐specific examination methylation, targeted high‐resolution methylation analysis, DNAge™ aging clock analysis with a translatable model voluntary murine endurance/resistance training (progressive weighted wheel running,...
Abstract With aging, skeletal muscle plasticity is attenuated in response to exercise. Here, we report that senescent cells, identified using senescence-associated β-galactosidase (SA β-Gal) activity and p21 immunohistochemistry, are very infrequent resting muscle, but emerge approximately 2 weeks after a bout of resistance exercise humans. We hypothesized these cells contribute blunted hypertrophic potential old age. Using synergist ablation-induced mechanical overload (MOV) the plantaris...
Myc is a powerful transcription factor implicated in epigenetic reprogramming, cellular plasticity, and rapid growth as well tumorigenesis. Cancer skeletal muscle extremely rare despite marked sustained induction during loading-induced hypertrophy. Here, we investigated global, actively transcribed, stable, myonucleus-specific transcriptomes following an acute hypertrophic stimulus mouse plantaris. With these datasets, define global Myc-specific dynamics at the onset of mechanical...
The presence of histone H3 lysine 36 methylation (H3K36me) correlates with actively transcribed genes. In yeast, H3K36me mediated by KMT3 (also known as Set2) recruits a deacetylase complex, Rpd3s, to ensure the fidelity transcription initiation. We report purification human KMT3a HYPB or hSet2) complex and identification novel, higher eukaryotic specific subunit, heterogeneous nuclear ribonucleoprotein L (HnRNP-L). Interestingly, although has intrinsic activity in vitro, HnRNP-L is...
Central neuroinflammation is important in the pathophysiological processes of neuropathic pain following peripheral nerve injury. Recently, interleukin-17 (IL-17) has been detected different inflammatory conditions central nervous system and contributes to associated with multiple sclerosis, experimental autoimmune encephalomyelitis. The present study, based on rat model spinal ligation, analyzed infiltration cluster differentiation (CD)4+ T cells expression IL‑17 cord during maintenance...
Abstract Using a mouse model of conditional and inducible in vivo fluorescent myonuclear labeling (HSA-GFP), sorting purification nuclei, low-input reduced representation bisulfite sequencing (RRBS), translatable reversible exercise (progressive weighted wheel running, PoWeR), we provide the first nucleus type-specific epigenetic information on skeletal muscle adaptation detraining. Adult (>4 mo) HSA-GFP mice performed PoWeR for 8 wk then detrained 12 wk; age-matched untrained were...
Key points Ribosome biogenesis and MYC transcription are associated with acute resistance exercise (RE) distinct from endurance in human skeletal muscle throughout a 24 h time course of recovery. A PCR‐based method for relative ribosomal DNA (rDNA) copy number estimation was validated by whole genome sequencing revealed that rDNA dosage is positively correlated ribosome response to RE. Acute RE modifies methylation patterns enhancer, intergenic spacer non‐canonical ‐associated regions, but...
The number of myonuclei within a muscle fiber is an important factor in growth, but its regulation during adaptation not well understood. We aimed to elucidate the time course myonuclear dynamics endurance training, loaded and concentric resistance nerve silencing-induced disuse atrophy with subsequent recovery. modified tibialis anterior activity free-living rats electrical stimulation from implantable pulse generators, or osmotic pumps delivering tetrodotoxin (TTX) silence motor without...
Murine exercise models can provide information on factors that influence muscle adaptability with aging, but few translatable solutions exist. Progressive weighted wheel running (PoWeR) is a simple, voluntary, low-cost, high-volume endurance/resistance approach for training young mice. In the current investigation, aged mice (22-mo-old) underwent modified version of PoWeR 8 wk. Muscle functional, cellular, biochemical, transcriptional, and myonuclear DNA methylation analyses an encompassing...
Abstract Exercise promotes functional improvements in aged tissues, but the extent to which it simulates partial molecular reprogramming is unknown. Using transcriptome profiling from (1) a skeletal muscle‐specific vivo Oct3/4 , Klf4 Sox2 and Myc (OKSM) reprogramming‐factor expression murine model; (2) an inducible induction (3) translatable high‐volume hypertrophic exercise training approach mice; (4) human muscle biopsies, we collectively defined exercise‐induced genes that are common...
Exercise is a potent stimulus for combatting skeletal muscle ageing. To study the effects of exercise on in preclinical setting, we developed combined endurance-resistance training mice called progressive weighted wheel running (PoWeR). PoWeR improves molecular, biochemical, cellular and functional characteristics promotes aspects partial epigenetic reprogramming when performed late life (22-24 months age). In this investigation, leveraged pan-mammalian DNA methylome arrays tandem...
Epigenetic regulation, including histone-to-protamine exchanges, controls spermiogenesis. However, the underlying mechanisms of this regulation are largely unknown. Here, we report that PHF7, a testis-specific PHD and RING finger domain-containing protein, is essential for exchange in mice. PHF7 specifically expressed during deletion results male infertility due to aberrant histone retention impaired protamine replacement elongated spermatids. Mechanistically, can simultaneously bind H2A H3;...
Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds myonuclei. Using progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization myonuclear transcription, we show functional specialization muscle. After 4 weeks training, snRNA-seq reveals resident stem cells, or satellite are activated with acute but demonstrate limited lineage progression while contributing to adaptation. In...
Muscle fibers are syncytial postmitotic cells that can acquire exogenous nuclei from resident muscle stem cells, called satellite cells. Myonuclei added to by during conditions such as load-induced hypertrophy. It is difficult dissect the molecular contributions of versus cell-derived myonuclei adaptation due complexity labeling distinct nuclear populations in multinuclear without label transference between nuclei. To sidestep this barrier, we used a genetic mouse model where myonuclear DNA...