A5101506029- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Ocular Surface and Contact Lens
- Air Quality and Health Impacts
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- RNA modifications and cancer
- Glaucoma and retinal disorders
- Air Quality Monitoring and Forecasting
- Ocular Diseases and Behçet’s Syndrome
- interferon and immune responses
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Heart Rate Variability and Autonomic Control
- Retinal Diseases and Treatments
- Genetics and Neurodevelopmental Disorders
- Climate Change and Health Impacts
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Histone Deacetylase Inhibitors Research
- Single-cell and spatial transcriptomics
- Noise Effects and Management
- Calcium signaling and nucleotide metabolism
Eye & ENT Hospital of Fudan University
2021-2024
State Key Laboratory of Medical Neurobiology
2024
Wenzhou-Kean University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2023
Memorial Sloan Kettering Cancer Center
2021-2023
Institute of Cancer Research
2023
Fudan University
2020-2023
Chinese Academy of Sciences
2017-2022
Institute of Biophysics
2017-2022
National Institute of Biological Sciences, Beijing
2010-2014
H3K27 methylation mediated by the histone methyltransferase complex PRC2 is critical for transcriptional regulation, Polycomb silencing, Drosophila segmentation, mammalian X chromosome inactivation, and cancer. PRC2-mediated can spread along chromatin propagate repressive environment; thus, components that antagonize activity of are important restraining silencing. Here we report in HeLa cells, H3 histones unmethylated at Lys-36 mostly methylated Lys-27, with exception newly synthesized H3....
Semiconservative DNA replication ensures the faithful duplication of genetic information during cell divisions. However, how epigenetic carried by histone modifications propagates through mitotic divisions remains elusive. To address this question, replication-dependent nucleosome partition pattern must be clarified. Here, we report significant amounts H3.3-H4 tetramers split in vivo, whereas most H3.1-H4 remained intact. Inhibiting deposition greatly reduced level splitting events, which...
Abstract Deciphering the mechanisms that control pluripotent ground state is key for understanding embryonic development. Nonetheless, epigenetic regulation of ground-state mouse stem cells (mESCs) not fully understood. Here, we identify protein MPP8 as being essential pluripotency. Its depletion leads to cell cycle arrest and spontaneous differentiation. has been suggested repress LINE1 elements by recruiting human silencing hub (HUSH) complex H3K9me3-rich regions. Unexpectedly, find are...
Bivalent genes are ready for activation upon the arrival of developmental cues. Here, we report that BEND3 is a CpG island (CGI)-binding protein enriched at regulatory elements. The cocrystal structure in complex with its target DNA reveals structural basis methylation-sensitive binding property. Mouse embryos ablated Bend3 died pregastrulation stage. null embryonic stem cells (ESCs) exhibited severe defects differentiation, during which hundreds CGI-containing bivalent were prematurely...
Nuclear receptor-binding SET-domain protein 1 (NSD1), a methyltransferase that catalyzes H3K36me2, is essential for mammalian development and frequently dysregulated in diseases, including Sotos syndrome. Despite the impacts of H3K36me2 on H3K27me3 DNA methylation, direct role NSD1 transcriptional regulation remains largely unknown. Here, we show are enriched at cis-regulatory elements, particularly enhancers. enhancer association conferred by tandem quadruple PHD (qPHD)-PWWP module, which...
Previously, we reported that little canonical (H3.1-H4)(2) tetramers split to form "hybrid" consisted of old and new H3.1-H(4) dimers, but approximately 10% (H3.3-H4)2 during each cell cycle. In this report, mapped the H3.3 nucleosome occupancy, turnover rate splitting events at genome-wide level. Interestingly, transcription starting sites (TSS) genes with different expression levels display a bimodal distribution rather than linear correlation towards transcriptional activity, suggesting...
Ash1 is a Trithorax group protein that possesses H3K36-specific histone methyltransferase activity, which antagonizes Polycomb silencing. Here we report the identification of two complex subunits, Mrg15 and Nurf55. In vitro, stimulates enzymatic activity Ash1. vivo, recruited by to their common targets, reinforces chromatin association facilitates proper deposition H3K36me2. To dissect functional role in context complex, identify an point mutation (Ash1-R1288A) displays greatly attenuated...
It remains unclear which size of particles has the strongest effects on heart rate variability (HRV). To explore association between HRV parameters and daily variations size-fractionated particle number concentrations (PNCs). We conducted a longitudinal repeated-measure study among 78 participants with 24-h continuous ambulatory Holter electrocardiographic recorder in Shanghai, China, from January 2015 to June 2019. Linear mixed-effects models were employed evaluate changes associated PNCs 7...
Glaucoma is the leading cause of irreversible vision loss worldwide, and reduction intraocular pressure (IOP) only factor that can be interfered to delay disease progression. As first line preferred method treat glaucoma, eye drops have many shortcomings, such as low bioavailability, poor patient compliance, unsustainable therapeutic effect. In this study, a highly efficient brimonidine (BRI) silicone rubber implant (BRI@SR@TPU implant) has been designed, prepared, characterized,...
Glaucoma is the leading cause of irreversible blindness, affecting 111 million people by 2040 worldwide. Intraocular pressure (IOP) only controllable risk factor for disease and current treatment options seek to reduce IOP via daily taking eye drops. However, shortcomings drops, such as poor bioavailability unsatisfied therapeutic effects, may lead inadequate patient compliance. In this study, an effective brimonidine (BRI)-loaded silicone rubber (SR) implant coated with polydimethylsiloxane...
Diabetic vasculopathy, encompassing complications such as diabetic retinopathy, represents a significant source of morbidity, with inflammation playing pivotal role in the progression these complications. This study investigates influence m6A modification and demethylase FTO on macrophage polarization its subsequent effects microvasculopathy. We found that diabetes induces shift towards pro-inflammatory M1 phenotype, which is associated reduction levels. Notably, emerges critical regulator...
Purpose: Autoimmune uveitis is a kind of sight-threatening ocular and systemic disorders. Recent treatments on autoimmune still remain many limitations due to extreme complexity undetermined pathogenesis. In this study, novel dual-drug nanocomposite formulation developed treat experimental by combined sustained therapy method. Methods: The constructed integrating berberine (BBR)-loaded mesoporous silica nanoparticles (MSNs) into dexamethasone (DEX)-loaded thermogel (BBR@MSN-DEX@Gel)....
Uveitis is a severe ocular inflammatory disease that affects the uvea and frequently results in visual impairment, even irreversible blindness. The current treatments for uveitis have exhibited adverse side effects. To find novel targets of this disease, we perform comparative transcriptome analysis using normal (n = 4) experimental autoimmune (EAU) rat iris samples. We mainly focus on expression profiles mRNAs long non-coding RNAs, identify NOD-like receptor signaling pathway as one plays...