A5101893588- Glycosylation and Glycoproteins Research
- Plant Gene Expression Analysis
- Galectins and Cancer Biology
- Plant biochemistry and biosynthesis
- Angiogenesis and VEGF in Cancer
- Plant Stress Responses and Tolerance
- Ecology and Conservation Studies
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Heat shock proteins research
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Ion channel regulation and function
- Molecular Biology Techniques and Applications
- Fungal and yeast genetics research
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Pancreatic and Hepatic Oncology Research
- Plant Micronutrient Interactions and Effects
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Skin and Cellular Biology Research
Wuhan YZY Biopharma (China)
2023-2025
Sun Yat-sen University
2019-2024
Yangzhou University
2015-2024
Ministry of Agriculture and Rural Affairs
2024
Guangdong Academy of Agricultural Sciences
2022-2024
Rice Research Institute
2024
Peking University
2016-2023
Oncode Institute
2020-2023
Leiden University Medical Center
2020-2023
ZheJiang Academy of Agricultural Sciences
2020
Recently, therapeutic antibodies against programmed cell death 1 (PD-1) and its ligand (PD-L1) have exerted potent anticancer effect in a variety of tumors. However, blocking the PD-1/PD-L1 axis alone is not sufficient to restore normal immune response. Other negative regulators antitumor immunity, like TGF-β VEGFA, are also involved escape tumor cells induce immunotherapy resistance.We developed novel anti-TGF-β/VEGF bispecific antibody Y332D based on Nano-YBODY™ technology platform. The...
Anthocyanin biosynthesis and sugar metabolism are important processes during plant growth, but the molecular interactions underlying these pathways still unclear. In this work, we analyzed anthocyanin soluble contents, as well transcript levels of transcription factors that known to be related in 'Hongcui 1' apple flesh fruit development. Overexpression MdMYB6 red-fleshed calli was found reduce content result downregulated expression MdANS MdGSTF12 proteins. Yeast one-hybrid electrophoretic...
Flesh lignification is a specific chilling response that causes deterioration in the quality of stored red-fleshed loquat fruit (Eribotrya japonica) and one aspect wider injury. APETALA2/ETHLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors are important regulators plant low-temperature responses lignin biosynthesis. In this study, expression action 27 AP2/ERF genes from cultivar 'Luoyangqing' were investigated order to identify regulating low-temperature-induced lignification. EjERF27,...
In many plants, anthocyanin biosynthesis is affected by environmental conditions. Ultraviolet-B (UV-B) radiation promotes accumulation and fruit coloration in apple skin, whereas high temperature suppresses these processes. this study, we characterized a B-box transcription factor, MdCOL4, from 'Fuji' apple, identified its role overexpressing encoding gene red callus. The expression of MdCOL4 was reduced UV-B, but promoted temperature. We explored the regulatory relationship between heat...
Abstract Non-alcoholic fatty liver disease (NAFLD) is the most common chronic worldwide, and development of non-alcoholic steatohepatitis (NASH) might cause irreversible hepatic damage. Hyperlipidemia (HLP) leading risk factor for NAFLD. This study aims to illuminate causative contributor potential mechanism Kallistatin (KAL) mediating HLP 221 healthy control 253 subjects, 62 44 NAFLD subjects were enrolled. The plasma KAL was significantly elevated in especially hypertriglyceridemia (HTG)...
Despite the success of immune checkpoint inhibitors (ICIs) in multiple malignant tumors, a significant proportion patients remain unresponsive to treatment. Radiotherapy (RT) elicits immunogenic antitumor responses but concurrently activates several evasion mechanisms. Our earlier research demonstrated efficacy YM101, an anti-TGF-β/PD-L1 bispecific antibody, stroma-rich tumors. Nevertheless, YM101 has reduced effectiveness non-inflamed tumors characterized by poor cell infiltration. This...
Ash1 is a Trithorax group protein that possesses H3K36-specific histone methyltransferase activity, which antagonizes Polycomb silencing. Here we report the identification of two complex subunits, Mrg15 and Nurf55. In vitro, stimulates enzymatic activity Ash1. vivo, recruited by to their common targets, reinforces chromatin association facilitates proper deposition H3K36me2. To dissect functional role in context complex, identify an point mutation (Ash1-R1288A) displays greatly attenuated...
Epithelial-mesenchymal transition (EMT) is pivotal in the initiation and development of cancer cell metastasis. We observed that abundance glycosphingolipids (GSLs), especially ganglioside subtypes, decreased significantly during TGF-β-induced EMT NMuMG mouse mammary epithelial cells A549 human lung adenocarcinoma cells. Transcriptional profiling showed TGF-β/SMAD response genes signatures were strongly enriched cells, along with depletion UDP-glucose ceramide glucosyltransferase (UGCG),...
Ubiquitin-specific protease (USP)19 is a deubiquitinating enzyme that regulates the stability and function of multiple proteins, thereby controlling various biological responses. The alternative splicing USP19 results in expression two major encoded variants are localized to endoplasmic reticulum (ER) (USP19-ER) cytoplasm (USP19-CY). importance for remains unclear. Here, we demonstrated USP19-CY promotes TGF-β signaling by directly interacting with type I receptor (TβRI) protecting it from...
Isoform 2 of the ryanodine receptor (RyR2) is major calcium release channel in cardiac muscle. In present study, two kinds RyR2 cDNA were constructed, one encoding wild type mouse (RyR2wt) and other modified RyR2, into which was inserted a green fluorescent protein (GFP). GFP divergent region 1 (DR1) after Asp-4365 (RyR2D4365-GFP). HEK293 cells expressing both RyR2wt RyR2D4365-GFPcDNAs showed caffeine- ryanodine-sensitive release, demonstrating that RyR2s form functional channels. Cells...
Recombinant type 3 ryanodine receptor (RyR3) has been purified in quantities sufficient for structural characterization by cryoelectron microscopy and three-dimensional (3D) reconstruction. Two cDNAs were prepared expressed HEK293 cells, one encoding the wild-type RyR3 other containing glutathione S-transferase (GST) fused to its amino terminus (GST-RyR3). was from detergent-solubilized transfected cells affinity chromatography using 12.6-kDa FK506-binding protein form of a GST fusion as...
Abstract Plant metabolites including anthocyanins play an important role in the growth of plants, as well regulating biotic and abiotic stress responses to environment. Here we report comprehensive profiling 3315 a further metabolic-based genome-wide association study (mGWAS) based on 292,485 SNPs obtained from 311 rice accessions, 160 wild 151 cultivars. We identified hundreds common variants affecting large number secondary with effects at high throughput. Finally, novel gene namely OsLSC6...
Changes in the glycosylation profile of cancer cells have been strongly associated with progression. To increase our insights into role human pancreatic ductal adenocarcinoma (PDAC), we performed a study on O-glycans and glycosphingolipid (GSL) glycans PDAC cell lines Pa-Tu-8988T (PaTu-T) Pa-Tu-8988S (PaTu-S). These are derived from same patient, but show an almost opposite phenotype, morphology capacity to metastasize, may thus provide attractive model progression PDAC. Gene-array analysis...
Lilium is an important commercial market flower bulb. qRT-PCR extremely technique to track gene expression levels. The requirement of suitable reference genes for normalization has become increasingly significant and exigent. internal control in living organisms varies considerably under different experimental conditions. For economically Lilium, only a limited number applied have been reported date. In this study, the stability 12 candidate including α-TUB, β-TUB, ACT, eIF, GAPDH, UBQ, UBC,...
Myo-inositol is a vital compound in plants. As the key rate-limiting enzyme myo-inositol biosynthesis, l-myo-inositol-1-phosphate synthase (MIPS) regarded as determinant of content The up-regulation MIPS genes can increase content, thereby enhancing plant's resistance to variety stresses. However, there are few reports on roles and identification woody trees. In this study, gene, named PeMIPS1, was characterized from Populus euphratica Oliv. heterologous expression PeMIPS1 compensated for...
Isovaleryl-CoA dehydrogenase (IVD), a member of the acyl-CoA (ACAD) family, is key enzyme catalyzing conversion isovaleryl-CoA to β-methylcrotonyl-CoA in third reaction leucine catabolism pathway and simultaneously transfers electrons electron-transferring flavoprotein (ETF) for ATP synthesis. We previously identified ETF ortholog rice blast fungus Magnaporthe oryzae (MoETF) showed that MoETF was essential fungal growth, conidiation pathogenicity. To further investigate biological function...
Activation of the unfolded protein response (UPR) signaling pathways is linked to multiple human diseases, including cancer. The inositol-requiring kinase 1α (IRE1α)-X-box binding 1 (XBP1) pathway most evolutionarily conserved three major branches UPR. Here, we performed a genome-wide siRNA screen obtain systematic assessment genes integrated in IRE1α-XBP1 axis. We monitored expression an XBP1-luciferase chimeric which luciferase was fused in-frame with spliced (active) form XBP1. Using...
Abstract The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves not only as a bicistronic message to translate core protein (Cp) and DNA polymerase (Pol), but also the template for reverse transcriptional replication viral upon packaging into nucleocapsid. Although it is well known that pgRNA translates much more Cp than Pol, molecular mechanism underlying regulation Pol translation efficiency from remains elusive. In this study, we systematically profiled HBV nucleocapsid-...