A5100332772- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Plant Molecular Biology Research
- Postharvest Quality and Shelf Life Management
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Heat shock proteins research
- Light effects on plants
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Seed Germination and Physiology
- Plant and Fungal Interactions Research
- Advanced Photocatalysis Techniques
- Endoplasmic Reticulum Stress and Disease
- Lignin and Wood Chemistry
- Cancer Research and Treatments
- Layered Double Hydroxides Synthesis and Applications
- Genomics, phytochemicals, and oxidative stress
- Salmonella and Campylobacter epidemiology
- Protein Structure and Dynamics
Shandong Agricultural University
2016-2025
Tianjin University of Science and Technology
2010-2024
Anhui Normal University
2024
State Key Laboratory of Synthetic Chemistry
2024
China Pharmaceutical University
2024
State Key Laboratory of Natural Medicine
2024
Research Institute of Forestry
2018-2023
Chinese Academy of Forestry
2018-2023
Jiangsu University
2010-2023
Jilin Agricultural University
2012-2023
Abstract Human selection has reshaped crop genomes. Here we report an apple genome variation map generated through sequencing of 117 diverse accessions. A comprehensive model speciation and domestication along the Silk Road is proposed based on evidence from genomic analyses. Cultivated apples likely originate Malus sieversii in Kazakhstan, followed by intensive introgressions M. sylvestris . Xinjiang China turns out to be “ancient” isolated ecotype not directly contributing domestication....
Flavonoids are major polyphenol compounds in plant secondary metabolism. Wild red-fleshed apples (Malus sieversii f. niedzwetzkyana) an excellent resource because of their much high flavonoid content than cultivated apples. In this work, R6R6, R6R1 and R1R1 genotypes were identified F1 segregating population M. niedzwetzkyana. Significant differences composition detected among the three by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis....
Summary In plants, flavonoids play critical roles in resistance to biotic and abiotic stresses, contribute substantially the quality, flavor, nutritional quality of many fruit crops. apple ( Malus × domestica ), inbreeding has resulted severe decreases genetic diversity flavonoid content. Over last decade, we have focused on improvement using wild red‐fleshed resources sieversii f . niedzwetzkyana ). Here, found that MYB transcription factors TF s) involved synthesis proanthocyanidins can be...
Auxin signaling, which is crucial for normal plant growth and development, mainly depends on ARF-Aux/IAA interactions. However, little known regarding the regulatory effects of auxin signaling anthocyanin metabolism in apple (Malus domestica). We investigated functions MdARF13, contains a repression domain localized to nucleus. This protein was observed interact with Aux/IAA repressor, MdIAA121, through its C-terminal dimerization domain. Protein degradation experiments proved that MdIAA121...
Abstract Ultraviolet‐B (UV‐B) radiation and low temperature promote the accumulation of anthocyanins, which give apple skins their red colour. Although many transcription regulators have been characterized in UV‐B low‐temperature pathways, interregulation synergistic effects are not well understood. Here, a B‐box factor gene, MdBBX20 , was identified to anthocyanin biosynthesis under conditions field experiments when overexpressed transgenic calli. The transcript level significantly induced...
Drought is an important environmental factor affecting the growth and production of agricultural crops fruits worldwide, including apple (Malus domestica). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles flavonoid synthesis flavonoid-mediated drought response mechanism remain elusive. In this study, we demonstrated that a drought-responsive HSF, designated MdHSFA8a, promotes accumulation flavonoids, scavenging reactive oxygen species, plant...
SUMMARY Sugar and anthocyanin are important indicators of fruit quality, understanding the mechanism underlying their accumulation is essential for breeding high‐quality fruit. We identified an R2R3‐MYB transcription factor MdMYB305 in red‐fleshed apple progeny, which was positively correlated with sugar content but negatively content. Transient injection, stable expression [overexpressing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)],...
Anthocyanins are valuable compounds in red-fleshed apples. The MdMYB10 transcription factor is an important regulator of the anthocyanin synthesis pathway. However, other factors key components complex network controlling and should be more thoroughly characterized. In this study, we used a yeast-based screening technology to identify MdNAC1 as that positively regulates synthesis. overexpression apple fruits calli significantly promoted accumulation anthocyanins. binding experiments,...
In plants, flavonoids are important secondary metabolites that contribute to the nutritional quality of many foods. Apple is a popular and frequently consumed food because its high flavonoid content. this study, composition content were detected compared between red- white-fleshed apples in BC1 hybrid population using ultraperformance liquid chromatography–quadrupole time-of-flight mass spectrometry. Transcriptomic analysis was then performed RNA-seq technology. By screening differentially...
Anthocyanin biosynthesis and sugar metabolism are important processes during plant growth, but the molecular interactions underlying these pathways still unclear. In this work, we analyzed anthocyanin soluble contents, as well transcript levels of transcription factors that known to be related in 'Hongcui 1' apple flesh fruit development. Overexpression MdMYB6 red-fleshed calli was found reduce content result downregulated expression MdANS MdGSTF12 proteins. Yeast one-hybrid electrophoretic...
Cold stress has always been a major abiotic factor affecting the yield and quality of temperate fruit crops. Ethylene plays critical regulatory role in cold response, but underlying molecular mechanisms remain elusive. Here, we revealed that ethylene positively modulates apple responses to stress. Treatment with 1-aminocyclopropane-1-carboxylate (an precursor) aminoethoxyvinylglycine biosynthesis inhibitor) respectively increased decreased tolerance seedlings. Consistent positive effects on...
Red-fleshed apple fruits are popular because of their high flavonoid content. Although MdMYB10 and its homologs have been identified as crucial regulators the fruit coloring process, other transcription factors (TFs) contributing to differences in flesh coloration not fully characterized. In this study, we investigated regulatory effects MdWRKY41 on anthocyanin proanthocyanidin (PA) synthesis red-fleshed apples. The overexpression calli inhibited PA accumulation by downregulating expression...
Anthocyanins are important secondary metabolites in plants. They for human health because of their antioxidant activities and dietary intake reduces the incidence cardiovascular cerebrovascular diseases tumors. The biosynthesis anthocyanins its regulation fruits vegetables is a global research hotspot. Compared with cultivated apples, red-fleshed apple relatively new popular commodity market. Previous studies on apples have focused basis high anthocyanin content transcriptional synthesis. In...
Abstract Enhancing fruit sugar contents, especially for high-flavonoid apples with a sour taste, is one of the main goals horticultural crop breeders. This study analyzed accumulation and underlying mechanisms in F2 progenies hybridization between high-sugar apple (Malus × domestica) variety “Gala” germplasm “CSR6R6”. We revealed that MdSWEET9b (sugars will eventually be exported transporter) helps mediate fruits. Functional characterization yeast mutants lacking transport as well...
Flavonoids are the most abundant class of secondary metabolites that ubiquitously involved in plant development and resistance to biotic abiotic stresses. Flavonoid biosynthesis involves multiple channels orchestrated molecular regulatory factors. Methyl jasmonate (MeJA) has been demonstrated enhance flavonoid accumulation numerous species; however, underlying mechanism MeJA-induced safflower is still not evident. In present study, we revealed basis a putative F3'5'H gene from imparting...
Escherichia coli contains the CspA family, consisting of nine proteins (CspA to CspI), in which CspA, CspB, and CspG have been shown be cold shock inducible CspD has stationary-phase inducible. The cspI gene is located at 35.2 min on E. chromosome map, CspI shows 70, 79% identity CspG, respectively. Analyses cspI-lacZ fusion constructs mRNA revealed that 5'-untranslated region consists 145 bases causes a negative effect expression 37 degrees C. was very unstable C but stabilized upon shock....
Summary Methylation at the MdMYB1 promoter in apple sports has been reported as a regulator of anthocyanin pathway, but little is known about how locus recognized by methylation machinery to regulate accumulation. In this study, we analysed three differently coloured ‘Fuji’ apples and found that differences transcript levels , which encodes key biosynthesis, control content (and therefore colour) fruit skin. The CHH MR3 region (−1246 −780) were be negatively correlated with expression. Thus,...