A5100460890- Nitric Oxide and Endothelin Effects
- Ion Channels and Receptors
- Eicosanoids and Hypertension Pharmacology
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Regulation of Appetite and Obesity
- Renin-Angiotensin System Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Irrigation Practices and Water Management
- ATP Synthase and ATPases Research
- Ion channel regulation and function
- Plant Molecular Biology Research
- Aquaculture disease management and microbiota
- Plant nutrient uptake and metabolism
- Blood Pressure and Hypertension Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammasome and immune disorders
- Molecular Sensors and Ion Detection
- Hydraulic flow and structures
- Endoplasmic Reticulum Stress and Disease
- Phosphodiesterase function and regulation
- Neuroscience of respiration and sleep
- Plant Reproductive Biology
- Marine Toxins and Detection Methods
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Peking Union Medical College Hospital
2025
Shandong Normal University
2019-2024
Northwest A&F University
2012-2023
University of Arizona
2006-2023
State Key Laboratory of Oncogene and Related Genes
2022
Shanghai Jiao Tong University
2022
Tang Du Hospital
2021
Air Force Medical University
2021
Jinzhou Medical University
2021
SUMMARY Sugar and anthocyanin are important indicators of fruit quality, understanding the mechanism underlying their accumulation is essential for breeding high‐quality fruit. We identified an R2R3‐MYB transcription factor MdMYB305 in red‐fleshed apple progeny, which was positively correlated with sugar content but negatively content. Transient injection, stable expression [overexpressing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)],...
A novel reversible two-photon fluorescent probe was developed, which well suited for the selective and instantaneous monitoring of HOCl levels, it successfully applied imaging levels in cells and<italic>in vivo</italic>.
Abstract The mechanism of pancreatic cancer (PA) is not fully understanded. In our last report, TRPM2 plays a promising role in cancer. However, the still unknown this dismal disease. This study was designed to investigate and overexpressed siRNA plasmid were created transfected with cell line (BxPC-3) construct model. We employed CCK-8, Transwell, scratch wound, nude mice tumor-bearing model Besides, we collected clinical data, tumor tissue sample (TT) para-tumor (TP) from patients treated...
Selective blockade of hypoxia-inducible gene expression by designed small molecules would prove valuable in suppressing tumor angiogenesis, metastasis and altered energy metabolism. We report the design, synthesis, biological evaluation a dimeric epidithiodiketopiperazine (ETP) molecule transcriptional antagonist targeting interaction p300/CBP coactivator with transcription factor HIF-1α. Our results indicate that disrupting this rapid downregulation genes critical for cancer progression....
Drug-induced liver injury (DILI) is the most common reason for post-marketing withdrawal of drugs. Poor understanding mechanisms DILI presents a large challenge in clinical diagnosis. Previous evidences indicate potential relationship between reactive nitrogen species (RNS) and DILI. Hence, we developed two specific probes, Golgi-HNO Mito-HNO, multicolored simultaneous situ imaging nitroxyl (HNO) Golgi apparatus mitochondria, respectively. We discovered significant rise HNO levels livers...
Acute lung injury (ALI) is a devastating clinical syndrome with no effective therapies. Inflammasome activation has been reported to play critical role in the initiation and progression of ALI. The molecular mechanisms involved regulating inflammasome ALI remains unresolved, although increases mitochondrial derived reactive oxygen species (mito-ROS) are involved. Our previous work shown that redistribution uncoupled eNOS impairs bioenergetics mito-ROS generation. Thus, focus our study was...
Atrial natriuretic factor (ANF) and nitric oxide (NO) stimulate production of guanosine 3',5'-cyclic monophosphate (cGMP) are natriuretic. Split-drop micropuncture was performed on anesthetized rats to determine the effects ANF NO donor sodium nitroprusside (SNP) proximal tubular fluid absorption rate (Jva). Compared with control solutions, SNP (10(-4) M) decreased Jva by 23% when administered luminally 35% added peritubular perfusate. Stimulation uptake luminal angiotensin II (ANG II;...
Mechanical ventilation is a life-saving intervention in critically ill patients with respiratory failure due to acute distress syndrome (ARDS), refractory lung disease an unacceptable high mortality rate. Paradoxically, mechanical also creates excessive stress that directly augments injury, known as ventilator-induced injury (VILI). The specific mechanisms involved VILI-induced pulmonary capillary leakage, key pathologic feature of VILI are still far from resolved. mechanoreceptor, transient...
Paeonia ostii is an important woody oil crop mainly cross-pollinated. However, the low yield has become factor restricting industrial development of P. ostii. Cross-pollination one measures to increase seed yield. Therefore, conservation pollen with high vitality crucial ensure successful pollination In this study, we found effective methodological system assess viability, ability germinate, and optimal storage conditions grains. The medium in vitro was 50 g/L sucrose, 100 mg/L boric acid,...
Aging is a natural process that affects the majority of organs within organism. The liver, however, plays pivotal role in maintaining organism's homeostasis due to its robust regenerative and metabolic capabilities. Nevertheless, liver also undergoes effects aging, which can result range disorders. function extracellular vesicles signals they convey represent significant area interest field ageing research. However, research on from perspective EVs remains relatively limited. In present...
Acute lung injury (ALI), a devastating illness induced by systemic inflammation e.g., sepsis or local COVID-19 mediated severe pneumonia, has an unacceptably high mortality and no effective therapy. ALI is associated with increased pulmonary microvascular hyperpermeability alveolar flooding. The small Rho GTPases, RhoA Rac1 are central regulators of vascular permeability through cytoskeleton rearrangements. have opposing functional outcome: induces endothelial contractile phenotype barrier...
Mitochondrial fission and a Warburg phenotype of increased cellular glycolysis are involved in the pathogenesis pulmonary hypertension (PH). The purpose this study was to determine whether increases mitochondrial glycolytic switch arterial endothelial cells (PAECs). is PAEC isolated from sheep model PH induced by overcirculation (Shunt PAEC). In Shunt we identified S
Small GTPases, particularly the Rho family, are key regulators of cell motility and migration. Dock180 was well known for main target signal adaptor protein Crk acted as a guanine-nucleotide exchange factor small GTPase Rac1. In present study, found to combine primarily with CrkI other than CrkII, its association Elmo1 also demonstrated in ovarian cancer SKOV3. To evaluate role human cell, we performed RNAi-mediated knockdown SKOV3 cells using interfering RNA expression vector. cells, that...
Capsaicin, the main constituent in chili, is an extremely spicy vanillin alkaloid and found several Capsicum species China. Traditionally, it has been used to treat inflammatory diseases such as allergic rhinitis, neuralgia after shingles, refractory female urethral syndrome, spontaneous recalcitrant anal pruritus, solid tumors. Constant stimulation of body by factors can lead chronic inflammation. Capsaicin possesses anti-inflammatory activity; however, underlying mechanism unknown. We...
Drip irrigation technology has obvious advantages in solving the shortage of agricultural water. The demand for using low-quality water sources is becoming urgent. Research on physical blockage formation become main part emitter China. purpose this study to investigate characteristics emitters drip system with a source and provide some basis anti-clogging measures. Therefore, we configured different concentrations (1.8 g/L, 2.8 3.8 g/L) particle sizes (0–0.054 mm, 0.054–0.075 0.075–0.1 mm)...
The transient receptor potential vanilloid type 1 (TRPV1) channel is a ligand-gated cation expressed by sensory nerves. P2Y receptors are G protein-coupled that also TRPV1-positive neurons. Therefore, we studied interactions between and TRPV1 function on kidney projecting Application of Fast Blue (FB) to nerves surrounding the renal artery retrogradely labeled neurons in dorsal root ganglia rats. Whole cell recording was performed FB-labeled maintained primary culture. Capsaicin used...
Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) play crucial roles in diabetic angiopathy. In vivo, however, the following facts remain unknown: whether COX-2 iNOS bind, how peroxynitrite-induced nitration of affects their binding if they do bind what effects this mechanism contribute to This study focused on issues above. Diabetes was induced Wistar male rats by intraperitoneal injection streptozotocin. As a specific scavenger peroxynitrite, urate used. After 13 wks...
This study tests the hypothesis that transient receptor potential vanilloid subtype 1 channel induced neuropeptide secretion and depressor response are mediated by, at least in part, activation of endoplasmic reticulum associated Ca release receptors, leading to increased cytosolic dorsal root ganglion neurons.
Oridonin, the main active constituent of Rabdosia rubescens, is known to exert antitumor activity via induction apoptosis in numerous types human cancer cells. However, underlying regulatory mechanisms mitochondrial ROS oridonin-induced HepG2 remain largely unknown, due limitations subcellular imaging resolution. Previously, it has been suggested that mitochondria serve a potential role sensing and signaling cellular redox changes vital biological processes such as cell death abiotic stress...