A5038820860- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Virus Research Studies
- Horticultural and Viticultural Research
- Phytochemicals and Antioxidant Activities
- Postharvest Quality and Shelf Life Management
- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Light effects on plants
- Plant Pathogenic Bacteria Studies
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Plant responses to elevated CO2
- Agriculture, Soil, Plant Science
- Mosquito-borne diseases and control
- Molecular Sensors and Ion Detection
- Bone Tissue Engineering Materials
Shandong Agricultural University
2016-2025
Qingdao University
2023-2025
Affiliated Hospital of Qingdao University
2023-2025
Ministry of Agriculture and Rural Affairs
2021-2024
China Agricultural University
2015-2024
University of Wisconsin–Madison
2019-2024
Shangqiu Normal University
2024
Nantong University
2022
Shandong Academy of Sciences
2022
Qilu University of Technology
2022
Abstract Human selection has reshaped crop genomes. Here we report an apple genome variation map generated through sequencing of 117 diverse accessions. A comprehensive model speciation and domestication along the Silk Road is proposed based on evidence from genomic analyses. Cultivated apples likely originate Malus sieversii in Kazakhstan, followed by intensive introgressions M. sylvestris . Xinjiang China turns out to be “ancient” isolated ecotype not directly contributing domestication....
Flavonoids are major polyphenol compounds in plant secondary metabolism. Wild red-fleshed apples (Malus sieversii f. niedzwetzkyana) an excellent resource because of their much high flavonoid content than cultivated apples. In this work, R6R6, R6R1 and R1R1 genotypes were identified F1 segregating population M. niedzwetzkyana. Significant differences composition detected among the three by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis....
Anthocyanins are biosynthesized on the cytosolic surface of endoplasmic reticulum and then transported into vacuole for storage. Glutathione S-transferases (GSTs) considered to be responsible transport anthocyanins vacuole. However, regulatory mechanisms GSTs in plants still unclear. Here, we performed a genome-wide analysis identified 69 GST genes apple. The expression MdGSTF6 was positively correlated with anthocyanin content (r = 0.949) during 'Yanfu 8' fruit development. overexpression...
Summary In plants, flavonoids play critical roles in resistance to biotic and abiotic stresses, contribute substantially the quality, flavor, nutritional quality of many fruit crops. apple ( Malus × domestica ), inbreeding has resulted severe decreases genetic diversity flavonoid content. Over last decade, we have focused on improvement using wild red‐fleshed resources sieversii f . niedzwetzkyana ). Here, found that MYB transcription factors TF s) involved synthesis proanthocyanidins can be...
Auxin signaling, which is crucial for normal plant growth and development, mainly depends on ARF-Aux/IAA interactions. However, little known regarding the regulatory effects of auxin signaling anthocyanin metabolism in apple (Malus domestica). We investigated functions MdARF13, contains a repression domain localized to nucleus. This protein was observed interact with Aux/IAA repressor, MdIAA121, through its C-terminal dimerization domain. Protein degradation experiments proved that MdIAA121...
Abstract Ultraviolet‐B (UV‐B) radiation and low temperature promote the accumulation of anthocyanins, which give apple skins their red colour. Although many transcription regulators have been characterized in UV‐B low‐temperature pathways, interregulation synergistic effects are not well understood. Here, a B‐box factor gene, MdBBX20 , was identified to anthocyanin biosynthesis under conditions field experiments when overexpressed transgenic calli. The transcript level significantly induced...
Drought is an important environmental factor affecting the growth and production of agricultural crops fruits worldwide, including apple (Malus domestica). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles flavonoid synthesis flavonoid-mediated drought response mechanism remain elusive. In this study, we demonstrated that a drought-responsive HSF, designated MdHSFA8a, promotes accumulation flavonoids, scavenging reactive oxygen species, plant...
Anthocyanins are valuable compounds in red-fleshed apples. The MdMYB10 transcription factor is an important regulator of the anthocyanin synthesis pathway. However, other factors key components complex network controlling and should be more thoroughly characterized. In this study, we used a yeast-based screening technology to identify MdNAC1 as that positively regulates synthesis. overexpression apple fruits calli significantly promoted accumulation anthocyanins. binding experiments,...
In plants, flavonoids are important secondary metabolites that contribute to the nutritional quality of many foods. Apple is a popular and frequently consumed food because its high flavonoid content. this study, composition content were detected compared between red- white-fleshed apples in BC1 hybrid population using ultraperformance liquid chromatography–quadrupole time-of-flight mass spectrometry. Transcriptomic analysis was then performed RNA-seq technology. By screening differentially...
Red-fleshed apples are popular as a result of their high anthocyanin content. MdMYB10 and its homologues known to be important regulators synthesis in apple, but the roles other transcription factors not well-understood. Here, we explored role MdWRKY11 regulating apple flesh. Overexpression callus could significantly promote accumulation, expression some MYB structural genes increased significantly. In binding analyses, bound W-box cis-elements promoters MdMYB10, MdMYB11, MdUFGT. However,...
Anthocyanin biosynthesis and sugar metabolism are important processes during plant growth, but the molecular interactions underlying these pathways still unclear. In this work, we analyzed anthocyanin soluble contents, as well transcript levels of transcription factors that known to be related in 'Hongcui 1' apple flesh fruit development. Overexpression MdMYB6 red-fleshed calli was found reduce content result downregulated expression MdANS MdGSTF12 proteins. Yeast one-hybrid electrophoretic...
Cold stress has always been a major abiotic factor affecting the yield and quality of temperate fruit crops. Ethylene plays critical regulatory role in cold response, but underlying molecular mechanisms remain elusive. Here, we revealed that ethylene positively modulates apple responses to stress. Treatment with 1-aminocyclopropane-1-carboxylate (an precursor) aminoethoxyvinylglycine biosynthesis inhibitor) respectively increased decreased tolerance seedlings. Consistent positive effects on...
Red-fleshed apple fruits are popular because of their high flavonoid content. Although MdMYB10 and its homologs have been identified as crucial regulators the fruit coloring process, other transcription factors (TFs) contributing to differences in flesh coloration not fully characterized. In this study, we investigated regulatory effects MdWRKY41 on anthocyanin proanthocyanidin (PA) synthesis red-fleshed apples. The overexpression calli inhibited PA accumulation by downregulating expression...
SUMMARY Sugar and anthocyanin are important indicators of fruit quality, understanding the mechanism underlying their accumulation is essential for breeding high‐quality fruit. We identified an R2R3‐MYB transcription factor MdMYB305 in red‐fleshed apple progeny, which was positively correlated with sugar content but negatively content. Transient injection, stable expression [overexpressing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)],...
Abstract Enhancing fruit sugar contents, especially for high-flavonoid apples with a sour taste, is one of the main goals horticultural crop breeders. This study analyzed accumulation and underlying mechanisms in F2 progenies hybridization between high-sugar apple (Malus × domestica) variety “Gala” germplasm “CSR6R6”. We revealed that MdSWEET9b (sugars will eventually be exported transporter) helps mediate fruits. Functional characterization yeast mutants lacking transport as well...
Abstract A library of new fluorescent molecular rotors (FMRs) for viscosity sensing was synthesized. The sensitivity the fluorescence emission toward solvent and polarity investigated by using UV/Vis absorption, spectra, theoretical calculations. For FMRs, red‐shifted emissions at 620 nm, Stokes shifts 170 up to 40‐fold enhancement upon increasing solvents were observed (cf. known FMRs with 491 shift 33 nm fivefold enhancement). By high but low polarity, example, polyethylene glycol...
Many plant viruses with monopartite or bipartite genomes have been developed as efficient expression vectors of foreign recombinant proteins. Nonetheless, due to lack multiple insertion sites in these viruses, it is still a big challenge simultaneously express proteins single cells. The genome Beet necrotic yellow vein virus (BNYVV) offers an attractive system for owning multipartite composed five positive-stranded RNAs. Here, we established BNYVV full-length infectious cDNA clone under the...
In many plants, anthocyanin biosynthesis is affected by environmental conditions. Ultraviolet-B (UV-B) radiation promotes accumulation and fruit coloration in apple skin, whereas high temperature suppresses these processes. this study, we characterized a B-box transcription factor, MdCOL4, from 'Fuji' apple, identified its role overexpressing encoding gene red callus. The expression of MdCOL4 was reduced UV-B, but promoted temperature. We explored the regulatory relationship between heat...