A5100460229- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Biological and pharmacological studies of plants
- Plant Pathogenic Bacteria Studies
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Legume Nitrogen Fixing Symbiosis
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Pharmacological Effects of Natural Compounds
- Traditional Chinese Medicine Studies
- interferon and immune responses
- Plant Genetic and Mutation Studies
- Genetics and Plant Breeding
Henan Agricultural University
2018-2024
Texas A&M University
2024
University of Michigan
2024
Guangzhou University of Chinese Medicine
2023
Hebei Agricultural University
2022
Fujian Agriculture and Forestry University
2015-2022
Chinese Academy of Agricultural Sciences
2012-2021
Biotechnology Research Institute
2012-2021
Fujian University of Traditional Chinese Medicine
2020-2021
Ministry of Agriculture and Rural Affairs
2021
Many plant viruses with monopartite or bipartite genomes have been developed as efficient expression vectors of foreign recombinant proteins. Nonetheless, due to lack multiple insertion sites in these viruses, it is still a big challenge simultaneously express proteins single cells. The genome Beet necrotic yellow vein virus (BNYVV) offers an attractive system for owning multipartite composed five positive-stranded RNAs. Here, we established BNYVV full-length infectious cDNA clone under the...
MicroRNAs (miRNAs) are small regulatory RNAs processed from primary miRNA transcripts, and plant miRNAs play important roles in growth, development, response to infection by microbes. Microbial infections broadly alter biogenesis, but the underlying mechanisms remain poorly understood. In this study, we report that Rice stripe virus (RSV)-encoded nonstructural protein 3 (NS3) interacts with OsDRB1, an indispensable component of rice (Oryza sativa) miRNA-processing complex. Moreover,...
In Arabidopsis thaliana, ARGONAUTE1 (AGO1) interacts with miR168 to modulate the small RNA regulatory pathway. However, underlying mechanism of regulation and relationship between AGO1 is poorly understood in cash crop Solanum lycopersicum (tomato). We previously found that SlAGO1A SlAGO1B were cleaved by tomato. this study, we show accumulate miR168-sponge transgenic plants, expression miR168-resistant (4m-SlAGO1A) (4m-SlAGO1B) tomato results a series defects affecting growth rate, floral...
Abstract Tilletia horrida is an important soilborne fungal pathogen that causes rice kernel smut worldwide. We found a glycoside hydrolase family 128 protein, designated ThGhd_7, caused cell death in Nicotiana benthamiana leaves. The predicted signal peptide (SP) of ThGhd_7 targets it for secretion. However, loss the SP did not affect its ability to induce death. 23–201 amino acid sequence was sufficient trigger N. . expression induced and upregulated during T. infection. localised both...
Nicotiana benthamiana is a model plant utilised internationally in virology because of its apparent hyper-susceptibility to virus infection. Previously, others showed that all laboratory accessions N. have very narrow genetic basis, probably originating from single source. It unknown if responses infection exhibited by the accession are typical species as whole. To test this, 23 were collected wild populations and challenged with one four viruses. Additionally, 21 other subspecies Australia,...
Abstract Intracellular potassium (K + ) transported by plants under the action of a number transport proteins is crucial for plant survival distinct abiotic and biotic stresses. A correlation between K status disease incidence has been found in many studies, but roles regulating resistance to viral diseases remain elusive. Here, we report that HIGH‐AFFINITY TRANSPORTER 5 (OsHAK5) regulates infection rice grassy stunt virus (RGSV), negative‐sense single‐stranded bunyavirus, ( Oryza sativa )....
To establish multi-class bioactive constituents' determination of ten Anoectochilus, four Goodyera and one Ludisia species, provide reference for the improvement their quality control.HPLC-ELSD phenol-sulphuric acid methods were used quantitative lactone glycosides (kinsenoside its diastereoisomer, goodyeroside A) polysaccharides, respectively, while an efficient iHPLC-MS/MS method was established rapid other minor constituents in Anoectochilus species five related species.The contents...
EP0 is an important early gene that modulates the life cycle of pseudorabies virus (PRV). A guanine-rich sequence overlapping with three Sp1 binding sites located upstream transcription start site (TSS) in promoter. Using native polyacrylamide gel electrophoresis (PAGE) and circular dichroism (CD), we verified G-rich region promoter forms intramolecular parallel G-quadruplex (G4) presence K+ ions. Further dimethyl sulphate (DMS) footprinting Taq polymerase stop assays indicates potential...
Viral suppressors of RNA silencing (VSRs) are a cluster viral proteins that have evolved to counteract eukaryotic antiviral pathways, thereby contributing pathogenicity. In this study, we revealed the matrix protein P4 encoded by rice stripe mosaic virus (RSMV), which is an emerging cytoplasmic rhabdovirus, weak suppressor. By conducting yeast two-hybrid, bimolecular fluorescence complementation, and subcellular colocalization assays, proved interacts with endogenous suppressor gene 3...
Ustilago crameri is a pathogenic basidiomycete fungus that causes foxtail millet kernel smut (FMKS), devastating grain disease in most foxtail-millet-growing regions of the world. Here, we report an assembled high-quality genome sequence U. strain SCZ-6 isolated from diseased grains Changzhi, Shanxi Province, China. The size 19.55 Mb, consisting 73 contigs (N50 = 840,209 bp) with G + C content 54.09%, and encoding 6576 predicted genes 6486 supported by RNA-seq. Evolutionarily, lies close to...
Summary Potassium (K + ) plays a crucial role as macronutrient in the growth and development of plants. Studies have definitely determined vital roles K response to pathogen invasion. Our previous investigations revealed that rice plants infected with grassy stunt virus (RGSV) displayed reduction content, but mechanism by which RGSV infection subverts uptake remains unknown. In this study, we found overexpression P1 , specific viral protein encoded RNA1, results enhanced sensitivity low...
Nucleolar proteins play important roles in plant cytology, growth, and development. Fibrillarin2 is a nucleolar protein of Nicotiana benthamiana ( N. ). Its cDNA was amplified by RT-PCR inserted into expression vector pEarley101 labeled with yellow fluorescent (YFP). The fusion localized the nucleolus Cajal body leaf epidermal cells . fibrillarin2 (NbFib2) has three functional domains (i.e., glycine arginine rich domain, RNA-binding α -helical domain) nuclear localization signal (NLS)...