A5069120331- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Respiratory viral infections research
- Virology and Viral Diseases
- Enzyme Production and Characterization
- Veterinary medicine and infectious diseases
- Herpesvirus Infections and Treatments
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Estrogen and related hormone effects
- Hepatitis B Virus Studies
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Glycosylation and Glycoproteins Research
- Vector-Borne Animal Diseases
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Influenza Virus Research Studies
Henan Agricultural University
2015-2024
Xinjiang Uygur Autonomous Region Disease Prevention and Control Center
2024
Shandong Agricultural University
2018-2023
Marine Biomedical Research Institute of Qingdao
2023
Sinopec (China)
2021
Ruijin Hospital
2021
China Animal Disease Control Center
2020
Lingnan Normal University
2017
World Wide Web Consortium
2011
Tianjin University
2010
Since May 2015, severe outbreaks of hepatitis-hydropericardium syndrome (HHS) associated with infections fowl aviadenovirus (FAdV) have emerged in broiler chickens several Chinese provinces. To identify the genotype and gain a better understanding genetic properties FAdV strains responsible for recent HHS China, complete genome sequences five isolates from provinces were determined. The results demonstrated that novel 4 (FAdV-4) was epidemic China. investigate molecular characteristics these...
Since May 2015, outbreaks of hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus 4 (FAdV-4) with a novel genotype have been reported in China, causing significant economic losses to the poultry industry. A previous comparative analysis revealed that highly virulent FAdV-4 isolates contain various genomic deletions and multiple distinct mutations major structural genes fiber2 hexon. To identify responsible for virulence HHS-associated isolates, infectious clones were...
Porcine epidemic diarrhea has re-emerged with devastating impact in central China since October 2010. To investigate and analyze the reason of this outbreak, M ORF3 genes 15 porcine viruses (PEDV), which were collected from different areas during 2010 December 2011, amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, analyzed. Sequence analyses showed that nucleotides amino acids changed at some sites PEDV strains compared those CV777 reference strain. Based on...
Shigellosis in chickens was first reported 2004. This study aimed to determine the pathogenicity of Shigella and possibility cross-infection between humans chickens. The examined via infection three-day-old SPF with strain ZD02 isolated from a human patient. virulence invasiveness were by chicken intestines primary intestinal epithelial cells. results showed can cause death intraperitoneal injection chickens, but only induce depression crop injection. Immunohistochemistry transmission...
Despite autophagy's pivotal role in the replication of viruses such as duck Tembusu virus (DTMUV), which has caused massive economic losses to poultry industry world, specific relationships between DTMUV and cellular autophagy remain largely unknown. In response, we investigated interactions DTMUV, effects structural non-structural proteins on autophagy, autophagy-related signaling pathways induced by DTMUV. Among results, increased flux embryo fibroblasts (DEF) BHK-21 cells, while...
A two-factor experiment was designed to determine the suitable dietary protein and lipid levels for juvenile Nibea diacanthus. Nine extruded pellet diets were formulated contain three of (420, 470 520 g kg−1) (70, 110 150 kg−1). Each diet randomly fed triplicate groups 25 N. diacanthus (initial weight 12.12 ± 0.23 g) 8 weeks in net cages. The results showed that gain rate (WGR), specific growth rate, final body energy retention significantly influenced by levels-. highest WGR (982.5g kg−1.)...
To the EditorSeneca Valley virus (SVV), an emerging non-enveloped, single-stranded RNA virus, belongs to genus Senecavirus in family Picornaviridae and has drawn a large amount of attention...
H9N2 avian influenza viruses (AIVs) have been detected from wild birds and domestic poultry worldwide. Serious diseases combined with secondary infection caused high mortality great economic losses to industry. Therefore, simple, rapid, sensitive accurate methods suitable for field detection of AIVs are crucial efficiently control virus spread in time. In this study, an isothermal reverse transcription recombinase polymerase amplification lateral-flow dipstick (RT-RPA-LFD) assay...
Interferon regulatory factor 1 (IRF1), as an important transcription factor, is abundantly induced upon virus infections and participates in host antiviral immune responses. However, the roles of porcine IRF1 (poIRF1) defense remain poorly understood. In this study, we determined that poIRF1 was upregulated infection with viruses distributed nucleus PK-15 cells. Subsequently, tested activities against several swine Overexpression can efficiently suppress replication viruses, knockdown...
Newcastle disease virus (NDV) and infectious bursal (IBDV) are the two most important widespread viruses causing huge economic losses in global poultry industry. Outbreaks of genotype VII NDV IBDV variants vaccinated flocks call for genetically matched vaccines. In present study, a genetic chimeric LaSota vaccine strain expressing VP2 gene variant, rLaS-VIIF/HN-VP2 was generated first time, which both F HN genes were replaced with those FJSW. The cleavage site FJSW protein genome mutated to...
Hepatitis-hydropericardium syndrome (HHS) induced by fowl adenovirus serotype 4 (FAdV-4) has caused huge economic losses to poultry industries. The key genes responsible for different virulence of FAdV-4 strains are not fully elucidated. Previous studies indicated that hexon pathogenic a conserved arginine (R) at position 188, and isoleucine (I) is present this in reported nonpathogenic FAdV-4. Recently, it was R188 the determinant site pathogenicity emerging Chinese strain. However, role...
Fowl adenovirus serotype 4 (FAdV-4) and FAdV-8b are causative agents of hepatitis-hydropericardium syndrome (HHS) inclusion body hepatitis (IBH), respectively. HHS IBH co-infections were often reported in clinical, yet there no commercially available bivalent vaccines for prevention control both FAdV-4 -8b. In the present study, a chimeric was firstly generated by substituting fiber-1 with fiber FAdV-8b. The virus, rFAdV-4-fiber/8b, exhibited similar replication ability vitro pathogenicity...
A quantitative PCR (qPCR) assay using SYBR Green I was developed based on the published sequence of gtxA gene from Gallibacterium anatis. This method produced reliable specificity, sensitivity, and repeatability. The detection rate in 181 clinical samples 36.5% (66/181) by qPCR, which superior to Gallibacterium-specific (0/181) an isolation identification (18.2% or 33/181). No association found between prevalence age chickens. infection detected one 4-day-old chicken, showing that can occur...