A5022798351- Muscle Physiology and Disorders
- Knee injuries and reconstruction techniques
- Sports injuries and prevention
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Total Knee Arthroplasty Outcomes
- Vitamin D Research Studies
- Cardiovascular and exercise physiology
- Osteoarthritis Treatment and Mechanisms
- Spaceflight effects on biology
- Tendon Structure and Treatment
- Muscle activation and electromyography studies
- Tissue Engineering and Regenerative Medicine
- Body Composition Measurement Techniques
- Musicians’ Health and Performance
- Sports Performance and Training
- Mesenchymal stem cell research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Cardiovascular Effects of Exercise
- Children's Physical and Motor Development
- Telomeres, Telomerase, and Senescence
University of Kentucky
2021-2025
Arizona State University
2019-2024
James Madison University
2020
Abstract With aging, skeletal muscle plasticity is attenuated in response to exercise. Here, we report that senescent cells, identified using senescence-associated β-galactosidase (SA β-Gal) activity and p21 immunohistochemistry, are very infrequent resting muscle, but emerge approximately 2 weeks after a bout of resistance exercise humans. We hypothesized these cells contribute blunted hypertrophic potential old age. Using synergist ablation-induced mechanical overload (MOV) the plantaris...
Murine exercise models can provide information on factors that influence muscle adaptability with aging, but few translatable solutions exist. Progressive weighted wheel running (PoWeR) is a simple, voluntary, low-cost, high-volume endurance/resistance approach for training young mice. In the current investigation, aged mice (22-mo-old) underwent modified version of PoWeR 8 wk. Muscle functional, cellular, biochemical, transcriptional, and myonuclear DNA methylation analyses an encompassing...
Abstract Molecular control of recovery after exercise in muscle is temporally dynamic. A time course biopsies around resistance (RE) combined with -omics necessary to better comprehend the molecular contributions skeletal adaptation humans. Vastus lateralis before and 30 minutes, 3-, 8-, 24-hours acute RE were collected. time-point matched biopsy-only group was also included. RNA-sequencing defined transcriptome while DNA methylomics computational approaches complemented these data. The...
Abstract The central dogma of molecular biology dictates the general flow information from DNA that leads to a functional cellular outcome. In skeletal muscle fibers, extent which global myonuclear transcriptional alterations, accounting for epigenetic and post-transcriptional influences, contribute an adaptive stress response is not clearly defined. this investigation, we leveraged integrated analysis myonucleus-specific methylome transcriptome, as well small RNA profiling molecularly...
Ground-based animal models have been used extensively to understand the effects of microgravity on various physiological systems. Among them, hindlimb suspension (HLS), developed in 1979 rats, remains gold-standard and allows researchers study consequences total unloading hind limbs while inducing a cephalic fluid shift. While this model has already brought valuable insights space biology, few studies directly compared functional decrements muscles males females during HLS. We exposed 28...
Musculoskeletal disorders contribute substantially to worldwide disability. Anterior cruciate ligament (ACL) tears result in unresolved muscle weakness and posttraumatic osteoarthritis (PTOA). Growth differentiation factor 8 (GDF8) has been implicated the pathogenesis of musculoskeletal degeneration following ACL injury. We investigated GDF8 levels ACL-injured human skeletal serum tested a humanized monoclonal antibody against placebo mouse model PTOA (surgically induced tear). In patients,...
ABSTRACT Background Blood flow restriction training (BFRT) is a popular rehabilitation intervention after anterior cruciate ligament reconstruction (ACLR). However, there are lack of clinical trials establishing the efficacy using BFRT during to improve quadriceps muscle function. Purpose The purpose this study evaluate blood strength, morphology, and physiology, knee biomechanics in individuals ACLR double-blind, randomized, placebo-controlled trial (NCT03364647). Methods Forty-eight...
Abstract Skeletal muscle adaptation to external stimuli, such as regeneration following injury and hypertrophy in response resistance exercise, are blunted with advanced age. The accumulation of senescent cells, along defects myogenic progenitor cell (MPC) proliferation, have been strongly linked contributing factors age-associated impairment adaptation. p53 plays an integral role all these processes, upregulation causes apoptosis cells prevents mitotic catastrophe MPCs from old mice. goal...
Background: Anterior cruciate ligament (ACL) tear (ACLT) leads to protracted quadriceps muscle atrophy. Protein turnover largely dictates size and is highly responsive injury loading. Regulation of molecular protein synthetic machinery after ACLT has been unexplored, limiting development targeted therapies. Purpose: To define the effect on (1) activation catabolic signaling within biopsy specimens from human participants (2) time course alterations synthesis its regulation in a mouse ACL...
Abstract Human hallmarks of sarcopenia include muscle weakness and a blunted response to exercise. Nicotinamide N-methyltransferase inhibitors (NNMTis) increase strength promote the regenerative capacity aged muscle, thus offering promising treatment for sarcopenia. Since human are recapitulated in (24-month-old) mice, we treated mice from 22 24 months age with NNMTi, intensive exercise, or combination both, compared skeletal adaptations, including grip strength, longitudinal running...
Knee osteoarthritis contributes substantially to worldwide disability. Post-traumatic (PTOA) develops secondary joint injury, such as ligament rupture, and there is increasing evidence suggesting a key role for inflammation in the aetiology of PTOA associated functional deficits. Colony stimulating factor 1 receptor (CSF1-R) has been implicated pathogenesis musculoskeletal degeneration following anterior cruciate (ACL) injury. We sought assess efficacy CSF1-R inhibition mitigate muscle...
BACKGROUND. Although 25-hydroxyvitamin D (25(OH)D) concentrations ≥30ng/mL are known to reduce injury risk and boost strength, the influence on anterior cruciate ligament reconstruction (ACLR) outcomes remains unexamined. This study aimed define vitamin signaling response ACLR, assess relationship between status muscle fiber cross-sectional area (CSA) bone density outcomes, discover receptor (VDR) targets post-ACLR.
Female athletes lag behind their male counterparts in recovery from anterior cruciate ligament (ACL) injury. Quadriceps muscle size and strength are crucial factors for regaining function after ACL injury, but little is known about how these metrics vary due to biological sex.
Abstract Objective To assess intrarater reliability of ultrasound‐determined measurements skeletal muscle characteristics across different measurement outcomes, imaging techniques, and age groups. Methods 2D ultrasound images (B‐mode) the quadriceps were obtained from young (26 ± 4 year, n = 8 M, F) older (70 7 5 adults on two occasions, separated by 6 3 days. With participants in both standing supine postures, collected five anatomical sites along anterior (two sites) lateral (three...
Satellite cells show consistent fusion into diaphragm muscle fibers throughout life, suggesting a critical role in maintaining homeostasis. Here, we report identical adaptations to hypoxia with and without satellite adult aged mice. In addition, propose that the higher number of Pax3-positive cell-depleted acts as compensatory mechanism.
Anterior cruciate ligament (ACL) tears are debilitating sport-related injuries resulting in protracted quadriceps atrophy. Our group has previously shown lower satellite cell abundance with atrophy following ACL injury. Canonically, cells fuse into muscle fibers to maintain myonuclear density and injury, reduced compromises recovery. We aimed determine whether this loss of is causative or a consequence PURPOSE: To the role recovery from METHODS: Pax7CreER/CreER:Rosa26DTA/DTA mice were...
PURPOSE: A reduction in satellite cells has been reported to contribute muscle loss with aging. Exercise presents a powerful strategy stimulate cells, however, what extent various forms of exercise skeletal older adults is less understood. The purposes this study were 1) examine relationships cell density, capillary and VO2peak adults, 2) identify changes density following two different intensities aerobic exercise. METHODS: In counter-balanced, cross-over design, six (4M, 2F; 67±2yr; BMI:...
PURPOSE: The purpose of this study was to determine if chocolate milk (CM) consumption after high-intensity cycling affected post-exercise recovery and subsequent exercise performance in youth cyclists, compared carbohydrate (CHO) placebo (PL) beverages. METHODS: Eight cyclists (15-18 y, VO2peak = 61.8±7.7 mL·kg-1·min-1) completed two sessions on three separate occasions. first session (EX1) included 30 min constant-load cycling, 60 standardized intervals. Subjects consumed a beverage (PL,...
Aging is associated with many physiological changes that impact physical function. Most notably, older adults experience a progressive loss of skeletal muscle mass and function, termed sarcopenia. A better understanding the molecular phenotypical advancing age may provide therapeutic targets for interventions to slow progression PURPOSE: To characterize transcriptional morphological profile aging muscle. METHODS: Resting vastus lateralis biopsies were collected from 9 young (Y; 27±3yr,...
Protracted quadriceps weakness and atrophy after anterior cruciate ligament (ACL) injury result in poor knee mechanics contribute to the development of posttraumatic osteoarthritis. The biological underpinnings functional recovery osteoarthritis are unknown, limiting evidence-based therapies. identification shared cellular pathology between skeletal muscle, bone articular cartilage would offer powerful therapeutic targets combat musculoskeletal degeneration; therefore, we sought define...