A5101878298- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- RNA regulation and disease
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Angiogenesis and VEGF in Cancer
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Nanoparticle-Based Drug Delivery
- Cell death mechanisms and regulation
- Cancer Mechanisms and Therapy
- Plant Virus Research Studies
- Cancer-related gene regulation
- Mycobacterium research and diagnosis
- interferon and immune responses
Chongqing Public Health Medical Center
2025
Chongqing Medical University
2025
Anhui Medical University
2025
Second Hospital of Anhui Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Institute of Hematology & Blood Diseases Hospital
2017-2024
Shanghai Ocean University
2024
Qingdao Agricultural University
2024
BGI Group (China)
2024
Henan University of Urban Construction
2024
Prognosis of patients with colorectal cancer (CRC) is generally poor because the lack simple, convenient, and noninvasive tools for CRC detection at early stage. The discovery microRNAs (miRNAs) their different expression profiles among kinds diseases has opened a new avenue tumor diagnosis. We built serum microRNA profile signature tested its specificity sensitivity as biomarker in diagnosis CRC. also studied possible role monitoring progression conducted two phase case-control test to...
Genome editing of human induced pluripotent stem cells (iPSCs) is instrumental for functional genomics, disease modeling, and regenerative medicine. However, low efficiency has hampered the applications CRISPR-Cas9 technology in creating knockin (KI) or knockout (KO) iPSC lines, which largely due to massive cell death after electroporation with plasmids. Here, we report that transient delivery BCL-XL increases survival by ∼10-fold plasmid transfection, leading a 20- 100-fold increase...
Analysis of global methylation in cells has revealed correlations between overall DNA status and some biological states. Recent studies suggest that epigenetic regulation through could be responsible for neuroadaptations induced by addictive drugs. However, there is no investigation to determine following repeated exposure Using mice conditioned place preference (CPP) procedure, we measured level the nucleus accumbens (NAc) prefrontal cortex (PFC) associated with drug rewarding effects. We...
Although the role of TNFAIP2 is still unclear, it an important gene involved in apoptosis, and there are single-nucleotide polymorphisms (SNPs) at its microRNA (miRNA)-binding sites that could modulate miRNA target function. In this study, we evaluated associations four selected SNPs (rs8126 T > C, rs710100 G A, rs1052912 A rs1052823 T) miRNA-binding 3′ untranslated region (UTR) with squamous cell carcinoma head neck (SCCHN) risk 1077 patients SCCHN 1073 cancer-free controls a non-Hispanic...
Concurrent chemoradiotherapy (CCRT) is the standard treatment for local advanced cervical cancer. However, elderly patients, studies are limited and outcomes controversial. We retrospectively analyzed efficacy tolerance of radical radiotherapy (RT) or CCRT in cancer patients performed comparisons between them. (≥70 years old) treated with RT January 2006 December 2014. For external beam radiotherapy, 50Gy 25 fractions 50.4Gy 28 were delivered via 3-dimensional conformal radiation therapy...
Hemophilia A, a bleeding disorder resulting from F8 mutations, can only be cured by gene therapy. A promising strategy is CRISPR-Cas9-mediated precise insertion of in hepatocytes at highly expressed loci, such as albumin (Alb). Unfortunately, the vivo integration efficiency long insert very low (~ 0.1%).We report that use double-cut donor leads to 10- 20-fold increase liver editing efficiency, thereby completely reconstituting serum activity mouse model hemophilia after hydrodynamic...
After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences.We show that deletions are ubiquitous but dependent on editing sites and cell types. Human primary T cells display more significant than hematopoietic stem progenitor (HSPCs), whereas we observe low levels in induced pluripotent (iPSCs). We find the homology-directed repair (HDR) with single-stranded oligodeoxynucleotides (ssODNs) carrying...
Ubiquitin C-terminal hydrolase-L1 (UCHL1) belongs to the family of deubiquitinating enzymes (DUBs), which is involved in ubiquitin-dependent proteolytic system. Previously, we have reported that upregulation UCHL1 related lymph node metastasis colorectal cancer (CRC). However, its molecular mechanisms remain elusive. In this study, transfected pcDNA3.1/UCHL1 and pcDNA3.1/UCHL1-C90S mutant into HCT8 cells. The changes biological features these stable transfectants were examined both vitro...
// Yuan Li 1, 2, 3 , Yan 2 Shengkai Huang Kun He 4 Mei Zhao Hong Lin Dongdong Jiaming Qian Caihong Zhou 5 Yuhua Chen Changzhi 1 State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Peking Union Medical College Chinese Academy Sciences, Beijing, 100021, China Department Etiology Carcinogenesis, Developmental Biology, University, Shenyang, 110122, Gastroenterology, 100730, Education, National Center/Cancer Sciences College, Correspondence to: Huang, email:...
An immunosuppressive microenvironment is critical for cancer initiation and progression. Whether interferon alpha (IFNα) can suppress immune cells its involved mechanism still remain largely elusive. We examine the expression of alpha/beta receptor-1 (IFNAR1), CD8, CD56 programmed death ligand 1 (PDL1) in head neck squamous cell carcinomas (HNSCC). The effect IFNα on PDL1 protein (PD1) tumour was detected vitro vivo. Overexpression IFNAR1, MX1 signal transducer activator transcription...
Abstract Mesoporous silica nanoparticles (MSN) can load and deliver potentially synergistic anticancer agents such as small molecule cytotoxics (like doxorubicin, DOX) nucleic acids microRNA, miRNA). However, these cargos have different underlying chemical properties so overcoming respective intracellular delivery barriers is a key consideration. Strategies to DOX from MSN frequently employ pH‐driven mechanisms that are restricted the acidic environment of lysosomes. Conversely, strategies...
Ubiquitin C-terminal hydrolase-L1 (UCHL1) is a de-ubiquitinating enzyme, which enzymatic activity relies on the C90 site. The function of UCHL1 controversial in different types cancer, and its role gastric cancer progression remains unclear. In this study, immunohistochemistry staining was applied to detect expression primary liver metastases from cancer. MKN45 BGC823 cell lines with stable de-ubiquitinase active or inactive UCHL1-variant C90S were established by lentiviral infection. effect...
Ubiquilin4 (Ubqln4), a member of the UbL-UBA protein family, serves as an adaptor in degradation specific substrates via proteasomal pathway. However, biological function Ubqln4 remains largely unknown, especially cancer. Here, we reported that was downregulated gastric cancer tissues and functioned tumor suppressor by inhibiting cell proliferation vivo vitro. Overexpression Ubqln4-induced cellular senescence G1-S cycle arrest cells activated p53/p21 axis. Moreover, regulated p21 through...
Caspase-8 (CASP8) is one of the most frequently mutated genes in head and neck squamous carcinomas (HNSCCs), CASP8 mutations are associated with poor survival. The distribution these HNSCCs suggests that they likely to be inactivating. Inhibition has been reported sensitize cancer cells necroptosis, a regulated cell death mechanism. Here, we show knockdown renders susceptible necroptosis by second mitochondria-derived activator caspase (SMAC) mimetic, birinapant, combination pan-caspase...
Checkpoint kinase inhibitors (CHKi) exhibit striking single-agent activity in certain tumors, but the mechanisms accounting for hypersensitivity are poorly understood. We screened a panel of 49 established human head and neck squamous cell carcinoma (HNSCC) lines report that nearly 20% hypersensitive to CHKi monotherapy. Hypersensitive cells underwent early S-phase arrest at drug doses sufficient inhibit greater than 90% CHK1 activity. Reduced rate DNA replication fork progression...
BACKGROUND:The aim of this study was to identify a panel serum noncoding RNAs (ncRNAs) as potential diagnostic and prognostic biomarkers for breast cancer. MATERIAL AND METHODS:Patients with cancer (n=30), normal controls (n=30) were included in the 'training set.' A 'validation set' cases (n=128) (n=77). All provided blood samples analysis. confirmed histologically staged. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) used detect expression 11 candidate ncRNAs,...
Long non-coding RNAs are thought to play a vital role in variety of human malignancies.Studies have shown that MIR155 host gene (MIR155HG) acts as an oncogene several cancers, but the function and its mechanism MIR155HG gastric cancer (GC) is still poorly understood.In this study, we determined biological functions underlying mechanisms GC cells.We found expression levels was increased markedly patients' serum.In vitro vivo studies demonstrated modulated malignant phenotype cells, such cell...
Abstract Background CRISPR-Cas9 technology has advanced in vivo gene therapy for disorders like hemophilia A, notably through the successful targeted incorporation of F8 into Alb locus hepatocytes, effectively curing this disorder mice. However, thoroughly evaluating safety and specificity is essential. Our study introduces a novel methodology to analyze complex insertion sequences at on-target edited locus, utilizing barcoded long-range PCR, CRISPR RNP-mediated deletion unedited alleles,...
Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. The effectors of phytopathogenic fungi play an important role during the infection process fungal pathogens onto their host plants. However, molecular mechanisms which R. regulate rice immunity are poorly understood. Through prediction, 78 candidate effector molecules were identified. Using tobacco rattle virus-host induced gene silencing system, 45 RNAi constructs genes infiltrated...
Metabolites are key indicators of health and therapeutic targets, but their genetic underpinnings during pregnancy-a critical period for human reproduction-are largely unexplored. Using data from non-invasive prenatal testing, we performed a genome-wide association study on 84 metabolites, including 37 amino acids, 24 elements, 13 hormones, 10 vitamins, involving 34,394 pregnant Chinese women, with sample sizes ranging 6,394 to 13,392 specific metabolites. We identified 53 metabolite-gene...
The study aimed to investigate the relationship between frequency of metabolic diseases (MetD) and dietary behaviors as well health risk in various age groups. This Cross-Sectional involved 12,215 participants, MetD was discussed using Pearson chi-square tests unordered binary logistics regression. Apriori algorithm used explore analyze combination patterns behaviors. Insufficient sleep a factor for (for young adults, odds ratio (OR): 1.31, 95% confidence interval (CI): 1.15, 1.50;...