A5112421461- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Heme Oxygenase-1 and Carbon Monoxide
- Helicobacter pylori-related gastroenterology studies
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Prenatal Substance Exposure Effects
- Respiratory viral infections research
- Gastroesophageal reflux and treatments
- Effects of Radiation Exposure
- Parvovirus B19 Infection Studies
- Virus-based gene therapy research
- Proteoglycans and glycosaminoglycans research
- Histiocytic Disorders and Treatments
- Synthesis and Characterization of Heterocyclic Compounds
- interferon and immune responses
- Fibroblast Growth Factor Research
- Advances in Cucurbitaceae Research
- Grief, Bereavement, and Mental Health
- Genetics and Neurodevelopmental Disorders
- Intracerebral and Subarachnoid Hemorrhage Research
- Hydrogen's biological and therapeutic effects
- Fluoride Effects and Removal
- Pharmacological Effects and Toxicity Studies
Chengdu University of Traditional Chinese Medicine
2024-2025
Sichuan Cancer Hospital
2025
University of Electronic Science and Technology of China
2025
Guizhou Center for Disease Control and Prevention
2024
Nanjing University
2023
Chinese Academy of Sciences
2023
Third Affiliated Hospital of Sun Yat-sen University
2021
Sun Yat-sen University
2021
Soochow University
2020
Second Affiliated Hospital of Soochow University
2020
Human immunodeficiency virus type 1 (HIV-1)-infected individuals who develop drug-resistant during antiretroviral therapy may derive benefit from continued treatment for two reasons. First, viruses can retain partial susceptibility to the drug combination. Second, selects that have reduced replication capacities relative archived, drug-sensitive viruses. We developed a novel single-cell-level phenotypic assay allows these effects be distinguished and compared quantitatively. Patient-derived...
Analysis of global methylation in cells has revealed correlations between overall DNA status and some biological states. Recent studies suggest that epigenetic regulation through could be responsible for neuroadaptations induced by addictive drugs. However, there is no investigation to determine following repeated exposure Using mice conditioned place preference (CPP) procedure, we measured level the nucleus accumbens (NAc) prefrontal cortex (PFC) associated with drug rewarding effects. We...
This study was conducted to evaluate whether dietary supplementation with L-arginine (Arg) could attenuate Escherichia coli LPS-induced liver injury through the TLR4 signaling pathway in weaned pigs. Eighteen pigs were allotted three treatments: non-challenged control, LPS challenged control and + 0.5% Arg. On d 18, injected at 100 µg/kg of body weight (BW) or sterile saline. Blood samples obtained 4 h post-injection. Pigs then sacrificed for collection samples. Arg (0.5%) alleviated...
Radiation-induced pulmonary fibrosis (RIPF) is a serious complication of radiotherapy that lacks effective treatment options. The Huaxian formula (HXF), traditional Chinese herbal remedy, shows promise in alleviating RIPF; however, its active ingredients and underlying mechanisms remain poorly understood. Through serum pharmacochemistry, network pharmacology, molecular docking, experimental validation, we investigate the potential HXF prevention radiation-induced (RIPF). Histological...
Although strides have been made to reduce ventilator-induced lung injury (VILI), critically ill patients can vary in sensitivity VILI, suggesting gene-environment interactions could contribute individual susceptibility. This study sought uncover candidate genes associated with VILI using a genome-wide approach followed by functional analysis of the leading mice. Alveolar-capillary permeability after high tidal volume (HTV) ventilation was measured 23 mouse strains, and haplotype association...
Adducin 1 (Add1) is known as a membrane cytoskeletal protein, but its nuclear function remains unclear. In this study, we generated add1-deficient zebrafish to investigate role in hematopoiesis. Lack of add1 impaired both primitive and definitive hematopoiesis, preventing healthy erythrocyte development. RNA sequencing revealed activation the p53 pathway add1-depleted erythroblast cells, leading apoptosis at 14-somites stage 24 hpf. Interestingly, partial rescue anemic phenotype was observed...
Abstract Background Intracerebral hemorrhage (ICH) seriously threatens the health of people. In addition, microglia M1 polarization was confirmed to be involved in progression ICH. Rosiglitazone able used as an antidiabetic agent, which could activate PPAR‐γ, and PPAR‐γ reported inhibit inflammation microglia. However, detailed function ICH remains unclear. Methods vivo vitro experiments were test RT‐qPCR western blot performed evaluate mRNA protein level respectively. Immunofluorescence...
The Huaxian formula (HXF), a traditional Chinese medicine (TCM) remedy, specifically targets the pathological factors of "heat toxicity" and "phlegm stasis" induced by radiation in radiation-induced pulmonary fibrosis (RIPF). It works clearing heat invigorating blood, addressing these key development RIPF.
Saquinavir (SQV) is the first FDA approved HIV protease inhibitor. Previous studies showed that SQV can limit Toll-like receptor-4 (TLR4)-mediated inflammatory pathway and nuclear factor-κB (NF-κB) activation, thereby playing a protective role in many kinds of diseases. High-mobility group box 1 (HMGB1) has been identified as an mediator it might express its toxicity short period time ventilator-induced lung injury (VILI). In this study, C57BL/6 mice were randomly divided into four groups (n...
In human macrophages and monocytes, lipopolysaccharide (LPS) induces nuclear factor kappa B (NFκB) activation pro-inflammatory cytokines production. We tested the possible involvement of Ku70 Ku80 in process. THP-1 primary peripheral blood mononuclear cells (PBMCs), shRNA-induced double knockdown potently inhibited LPS-induced production (TNF-α, IL-1β IL-6). Additionally, we developed CRISPR/Cas-9 gene-editing methods to knockout both PBMCs. Double (DKO) largely Conversely, exogenous...
Neural tube defects (NTDs) are associated with significant infant morbidity and mortality. Detection of alterations in DNA methylation might provide evidence for an epigenetic mechanism some NTDs cases. We developed a sensitive LC–MS/MS method the quantification global low quantity samples embryonic tissue. The limit detection was 0.5 × 10−15 mol 5-methyl-2′-deoxycytidine (5mdC) assay linear from 1 10−14 to 10−11 5mdC input between ng 1000 ng. calibration curve ranged 1% 10% correlation...
Simultaneous quantification of 9 pivotal amino acid metabolites in neural tube defect tissues using a LC-MS/MS method.
Objective To investigate the clinical implication of decorin expression in cholangiocarcinoma.Methods The levels were detected by immunohistochemistry 44 cases cholangiocarcinoma and 40 normal bile duct tissues.The relationship between level with sex,age,tumor cell differentiation lymph node metastasis patients was analyzede.Results Decorin down-regulated human cholangiocarcinomas cancer tissues.Decorin protein positive tumor interstitial tissues staining weaker than adjacent noncancerous...