Disrupted iron homeostasis in the substantia nigra pars compacta (SNpc) is an important pathological mechanism Parkinson’s disease (PD). It unclear what role microglia play metabolism and selective deposition SNpc of PD brain. In this study, we observed that 6-hydroxydopamine (6-OHDA) induced expression divalent metal transporter-1 (DMT1) influx BV2 cells, which might be associated with upregulation regulatory protein 1 (IRP1) expression. Moreover, found 6-OHDA had no significant effect on...

The process of an intracranial aneurysm development, growth, and rupture is multifaceted complex. In addition, clinical observations have identified the potential thrombus formation within such aneurysms. While underlying mechanism not fully understood, thrombi represent a risk factor for ischemic stroke. Emerging studies indicate that blood residence time (RT) promising hemodynamic metric associated with intra-aneurysmal thrombi. Here, we present methodology to experimentally evaluate both...

Recombinant derivatives of tetanus toxin (TeTx) were isolated and used to immunize mice. TeTx light chain, a derivative fragment C that had lost the ability bind neurons, recombinant holotoxoid could protect mice against challenge identified.

Abstract Background Intracerebral hemorrhage (ICH) seriously threatens the health of people. In addition, microglia M1 polarization was confirmed to be involved in progression ICH. Rosiglitazone able used as an antidiabetic agent, which could activate PPAR‐γ, and PPAR‐γ reported inhibit inflammation microglia. However, detailed function ICH remains unclear. Methods vivo vitro experiments were test RT‐qPCR western blot performed evaluate mRNA protein level respectively. Immunofluorescence...

Dystonia is a neurological movement disorder characterized by repetitive, unintentional movements and disabling postures that result from sustained or intermittent muscle contractions. The basal ganglia cerebellum have received substantial focus in studying DYT1 dystonia. It remains unclear how cell-specific ∆GAG mutation of torsinA within specific cells the affects motor performance, somatosensory network connectivity, microstructure. In order to achieve this goal, we generated two...

Alpha-synuclein (α-syn) is a major component of Lewy bodies, which biomarker Parkinson's disease (PD). It accumulates in substantia nigra pars compacta (SNpc) to form insoluble aggregates and cause neurotoxicity, often accompanied by iron deposition. We compared the reductase activity between monomeric α-syn (M-α-syn) oligomeric (O-α-syn) investigated effect on metabolism BV2 microglia cells as well. had ferric activity, O-α-syn stronger enzyme than M-α-syn. M-α-syn upregulated uptake...

To analyze the application of transabdominal sonography and transvaginal in early diagnosis pregnancy to provide a reference for selection methods clinic.One hundred eighty patients who were admitted into hospital from February 2013 2014, received clinical confirmed as ectopic gestation by surgery selected research objects. They examined sonography, combination with respectively. Besides, ultrasonic performance was observed compared surgical pathological results compare diagnostic accordance...

Abstract Background The initial CT blend sign is an imaging marker that has been used to predict haematoma expansion and poor outcomes in patients with small-volume intracerebral haemorrhage (ICH). However, the association of undergoing surgery remains unclear. present study aimed retrospectively evaluate influence on short-term hypertensive ICH after stereotactic minimally invasive (sMIS). Methods We enrolled 242 spontaneous ICH. were assigned group (91 patients) or non-blend (control) (151...

Alpha-synuclein (α-syn) is a major component of lewy bodies, which biomarker Parkinson's disease (PD). It accumulates in substantia nigra pars compacts (SNpc) to form insoluble aggregates and cause neurotoxicity, often accompanied by iron deposition. In this study, we compared the reductase activity between monomeric α-syn (M-α-syn) oligomeric (O-α-syn), investigated effect on metabolism BV2 microglia cells as well. We found that had ferric activity, O-α-syn stronger enzyme than M-α-syn....

Objective: To observe the effects of minimally invasive surgery (MIS) for hematoma intracerebral hemorrhage (ICH) evacuation followed by rosiglitazone (RSG) infusion therapy on perihematomal tight junction associated proteins occludin and zonula occludens-1 (ZO-1) expression as well blood brain barrier (BBB) permeability in rabbits. Methods: A total 50 male rabbits (2.5-3.5 kg) were randomly assigned to a sham group (Sham group, 10 rabbits), an ICH model (HM RSG medication (RSG (MIS rabbits)...

OBJECTIVE: To determine the relationship of nutritional status and MJ use in HIV+ population . METHODS: Dietary intake, food insecurity, plasma levels nutrients, anthropometrics, CD4 count, viral load ART were determined with consent 201 participants. Multivariate logistic regressions used. RESULTS : Mean age was 45.2 years, mostly males (64%) Blacks (72.5%); 27% used MJ. users younger (mean 42 vs. 46, P <0.001) alcohol tobacco more frequently than non‐users (94% 87%, <0.001)....

Abstract Backgrounds: The initial CT blend sign has been used as an imaging maker to predict haematoma expansion and poor outcome in patients with small volume intracerebral haemorrhage (ICH). However, the relationship between of remains elusive. present study aimed retrospectively observe impact signs on short-term hypertensive ICH underwent a stereotatic minimally invasive surgery (sMIS). Methods: Two hundreds forty-two spontaneous were enrolled. Based features, assigned group (including...