A5005437380- Radioactivity and Radon Measurements
- Animal testing and alternatives
- Hematopoietic Stem Cell Transplantation
- Radiation Dose and Imaging
- Effects of Radiation Exposure
- Mesenchymal stem cell research
- Trace Elements in Health
- Radioactive contamination and transfer
- Air Quality and Health Impacts
- Drug Transport and Resistance Mechanisms
- Ethics in Clinical Research
- Immunotoxicology and immune responses
- Radiation Therapy and Dosimetry
- Agriculture and Rural Development Research
- Inhalation and Respiratory Drug Delivery
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Cephalopods and Marine Biology
- Pharmacogenetics and Drug Metabolism
- Veterinary Pharmacology and Anesthesia
- Carcinogens and Genotoxicity Assessment
- Acute Myeloid Leukemia Research
- Agricultural safety and regulations
- Veterinary Practice and Education Studies
- Immune Response and Inflammation
Sanofi (France)
2008-2025
Institut de Radioprotection et de Sûreté Nucléaire
2002-2008
Service de la Santé Publique
2006-2007
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1998-2001
CEA DAM Île-de-France
1999
Abstract Background Recent studies have suggested that ex vivo expansion of autologous hematopoietic cells could be a therapy choice for the treatment bone marrow failure. We investigated potential combined infusion expanded with mesenchymal (MSCs) multi‐organ failure syndrome following irradiation in non‐human primate model. Methods Hematopoietic and MSCs were from aspirates. transduced gene encoding green fluorescent protein (e‐GFP), order to track them infusion. Twelve animals studied....
Kidney disease is a frequent consequence of heavy metal exposure and renal anemia occurs secondarily to the progression kidney deterioration into chronic disease. In contrast, little known about effects on low levels depleted uranium (DU). Study was performed with rats exposed DU at 40 mg/l by ingestion during 9 months. present work, ∼20% reduction in red blood cell (RBC) count observed after exposure. Hence, three hypotheses were tested determinate origin RBC loss: (1) reduced...
How do you promote ethical principles on a global scale regarding the use of animals for scientific purposes within pharmaceutical company like Sanofi? In 2017, Advisory Body Animal Ethics (ABAE) was created to harmonize animal ethics across all Sanofi sites. As an advisory body Bioethics Committee, ABAE addresses societal concerns related and develops corporate policies critical topics. Its composition, objectives operating processes are described, along with results achieved. Emphasis is...
Directive 2010/63/EU was adopted in September 2010 by the European Parliament and Council, became effective January 2013. It replaces 86/609/EEC introduces new requirements for protection of animals used scientific purposes. In particular, it requires that establishments breed, supply or use laboratory have a designated veterinarian (DV) with expertise animal medicine, suitably qualified expert where more appropriate, charged advisory duties relation to well-being treatment animals. This...
Bertho, J-M., Prat, M., Frick, J., Demarquay, C., Gaugler, M-H., Dudoignon, N., Clairand, I., Chapel, A., Gorin, N-C., Thierry, D. and Gourmelon, P. Application of Autologous Hematopoietic Cell Therapy to a Nonhuman Primate Model Heterogeneous High-Dose Irradiation. Radiat. Res. 163, 557– 570 (2005).We developed model heterogeneous irradiation in nonhuman primate test the feasibility autologous hematopoietic cell therapy for treatment radiation accident victims. Animals were irradiated...
We evaluated the possibility of using plasma Flt3 ligand (FL) concentration as a biological indicator bone marrow function after heterogeneous irradiation. Mice were irradiated with 4, 7.5 or 11 Gy 25, 50, 75 100% in field This model irradiation resulted graded and controlled damage to marrow. exhibited pancytopenia correlated both radiation dose percentage irradiated. The FL blood increased severity aplasia. Nonlinear regression analysis showed that was strongly total number residual...
The aim was to determine the gastrointestinal segments preferentially implicated in absorption of uranium. apparent permeability uranium (233U) measured ex vivo Ussing chambers assess passage various parts small and large intestines. transepithelial electrical parameters (potential difference, short-circuit current, resistance tissue conductance) were also recorded for each segment. Determination after in-situ deposition 233U digestive (buccal cavity, ileum proximal colon) measurements...
In the event of ingestion, digestive tract is first biological system exposed to depleted uranium (DU) intake via intestinal lumen. However, little research has addressed consequences a contamination with on properties such as barrier function and/or immune status this tissue. The aim study was determine if ingestion led changes in gut intestine. experiments were performed at 1 and 3 d following per os administration DU rats sublethal dose (204 mg/kg). Several parameters referring status,...
To assess the therapeutic efficacy of ex vivo-expanded hematopoietic cells in treatment radiation-induced pancytopenia, we have set up a non-human primate model. Two vivo expansion protocols for bone marrow mononuclear (BMMNC) were studied. The first consisted 7-day culture presence stem cell factor (SCF), Flt3-ligand, thrombopoietin (TPO), interleukin-3 (IL-3), and IL-6, which induced preferentially immature [3.1 +/- 1.4, 10.0 5.1, 2.2 1.9, 1.0 0.3-fold (MNC), colony-forming...
Purpose : To compare survival, lung dosimetry and gross pathology after inhalation exposure of rats to either NpO 2 or industrial PuO aerosols with similar granulometric parameters. Because the specific alpha activity ratio Pu/Np is about 600, a much more homogeneous irradiation was expected for 2. Materials methods Male Sprague Dawley were exposed once their burdens measured by X-ray spectrometry at different times post-exposure up death. The time-course doses delivered lungs estimated,...
Journal Article Does mean lung dose calculated after inhalation of alpha emitters actually reflect the risk induction malignant tumours? Get access P. Fritsch, Fritsch Search for other works by this author on: Oxford Academic PubMed Google Scholar N. Dudoignon, Dudoignon K. Guillet, Guillet Y. Oghiso, Oghiso J. Morlier, Morlier G. Monchaux Radiation Protection Dosimetry, Volume 105, Issue 1-4, 1 July 2003, Pages 149–152, https://doi.org/10.1093/oxfordjournals.rpd.a006211 Published: 01 2003
The tumor suppressor gene Tp53 was analyzed by polymerase chain reaction-amplification of genomic DNA extracted from paraffin-embedded tissue sections rat lung tumors to compare mutations that occurred after inhalation exposures plutonium dioxide, neptunium or radon and progenies. Exons 5 8 the were amplified in 16 plutonium-, 23 neptunium- 15 radon-induced tumors, their reaction products examined for single strand conformational polymorphism analysis direct sequencing method. Two point...
Purpose : To compare the incidence of each lung tumour type after inhalation exposure rats to either NpO 2 or industrial PuO aerosols, which have a similar size. Materials and methods Male Sprague-Dawley were exposed once followed during their whole life span. At end life, lungs fixed, embedded cut into thin sections for histological analysis. The presence tumours was evaluated on three distinct levels lobes phenotype determination establish dose-effect relationships. Results In range doses...
This study aimed to correlate blood Flt3-ligand (FL) concentration with CD34+ cell number in and bone marrow (BM) during granulocyte colony-stimulating factor (G-CSF) mobilization. Nonhuman primates were injected 10 μg/kg of G-CSF (Lenograstim) daily over a period 5 days. Daily sampling repeated BM showed that FL before mobilization was negatively correlated the absolute cells, but also G-CSF-mobilized cells on days 3–5 treatment. reflected status mobilization, suggested this parameter could...
As part of the improvement calibration techniques for in vivo counting, Laboratory Internal Dose Assessment IRSN has developed a computer tool, “OEDIPE”, to model internal contamination voxel phantoms, simulate counting and calculate dose. This software was used real distribution activity lungs baboon analyzed Radiotoxicology CEA. experiment provided opportunity study influence heterogeneity lung retention on quantify its effect assessment by comparison with simulated homogeneous same total...
The aim of this study was to assess the potential gastrointestinal peptide plasma levels as biomarkers radiation-induced digestive tract damage. To end, substance P, GRP, motilin, PYY, somatostatin-28, gastrin, and neurotensin were followed for up 5 days in pigs after a 16-Gy whole-body X-irradiation, completed by histopathological performed at days. Each gave specific response irradiation. GRP P not modified irradiation exposure; neither those motilin PYY. Concerning 23-fold increase...
De nombreuses études suggèrent que les cellules souches adultes et plus particulièrement Mésenchymateuses humaines (CSMh) pourraient être utilisées pour réparer de nombreux organes.Nous avons étudié la capacité des CSMh à réduire lésions cutanées radio induite.Pour induire sévères peau, souris NOD/SCID ont été irradiées au niveau patte droite postérieure (30 Gy, débit 2,7 Gy/mn) en utilisant une source gamma 60 Co.Les injectées 24 heures après irradiation par voie intraveineuse.La présence...
The results of several studies experimental carcinogenesis suggest that, after inhalation alpha-particle emitters, lung tumor incidence varies depending on the exposure rate and dose distribution in tissue. In case transuranics, main influencing factor would be specific activity inhaled actinide. To confirm these results, long-term were performed using male Sprague-Dawley rats exposed to (237)NpO(2) by inhalation. initial burdens animals ranged from 0. 1 about 7 kBq. followed during their...