Abstract Thermal tolerance is a critical determinant of ectotherm distribution, which likely to be influenced by future climate change. To predict such distributional changes, simple and comparable measures heat are needed these should ideally correlate with the characteristics species current thermal environments. A recent model (thermal landscapes—TTLs) uses exponential relation between temperature knockdown time describe ectotherms across time/temperature scales. Here, we established TTLs...

Upper thermal limits (CTmax) are frequently used to parameterize the fundamental niche of ectothermic animals and infer biogeographical distribution under current future climate scenarios. However, there is considerable debate associated with methodological, ecological physiological definitions CTmax. The recent (re)introduction death time (TDT) model has reconciled some these issues now offers a solid mathematical foundation CTmax by considering both intensity duration stress. Nevertheless,...

Abstract Temperature tolerance is critical for defining the fundamental niche of ectotherms and researchers classically use either static (exposure to a constant temperature) or dynamic (ramping assays assess tolerance. The different methods complicates comparison between studies here we present mathematical model (and R -scripts) reconcile thermal measures obtained from assays. Our uses input data several experiments based on well-supported assumption that injury accumulation rate increases...

Mitochondria have been suggested to be paramount for temperature adaptation in insects. Considering the large range of environments colonized by this taxon, we hypothesized that species surviving changes would those with most flexible mitochondria. We thus investigated responses mitochondrial oxidative phosphorylation (OXPHOS) three flying insects: honeybee (Apis mellifera carnica), fruit fly (Drosophila melanogaster) and Colorado potato beetle (Leptinotarsa decemlineata). Specifically,...

The spotted wing drosophila (SWD), Drosophila suzukii, is a major invasive fruit pest. There strong consensus that low temperature among the main drivers of SWD population distribution, and invasion success also linked to its thermal plasticity. Most studies on ectotherm cold tolerance focus exposure single stressful but here we investigated how stress intensity affected survival duration across broad range temperatures (-7 +3 °C). analysis Lt50 at different (Thermal Death Time curve - TDT)...

ABSTRACT Ectotherm thermal tolerance is critical to species distribution, but at present the physiological underpinnings of heat remain poorly understood. Mitochondrial function perturbed critically high temperatures in some ectotherms, including insects, suggesting that these animals linked failure oxidative phosphorylation (OXPHOS) and/or ATP production. To test this hypothesis, we measured mitochondrial oxygen consumption rate six Drosophila with different using high-resolution...

When heated, insects lose coordinated movement followed by the onset of heat coma (critical thermal maximum, CTmax). These traits are popular measures to quantify interspecific and intraspecific differences in insect tolerance, CTmax correlates well with current species distributions insects, including Drosophila Here, we examined function central nervous system (CNS) five different tolerances, while they were exposed either constant high temperature or a gradually increasing (ramp)....

ABSTRACT The ability of ectothermic animals to live in different thermal environments is closely associated with their capacity maintain physiological homeostasis across diurnal and seasonal temperature fluctuations. For chill-susceptible insects, such as Drosophila, cold tolerance tightly linked ion water obtained through a regulated balance active passive transport. Active transport at low requires constant delivery ATP we therefore hypothesize that cold-adapted Drosophila are...

Numerous assays are used to quantify thermal tolerance of arthropods including dynamic ramping and static knockdown assays. The assay measures a critical temperature while the animal is gradually heated, whereas time at constant temperature. Previous studies indicate that heat measured by both can be reconciled using × interaction from "thermal landscapes" (TTLs) in unhardened animals. To investigate if this relationship remains true within hardened animals, we use assess effect hardening...

Abstract When heated, insects loose coordinated movement followed by the onset of heat coma (CT max ). These phenotypes are popular measures to quantify inter- and intraspecific differences in insect tolerance, CT correlate well with current species distributions. Here we examined function central nervous system (CNS) five Drosophila different tolerances, while they were exposed either constant high temperature or a gradual increasing (ramp). Tolerant able preserve CNS at higher temperatures...

Abstract Temperature tolerance is critical for defining the fundamental niche of ectotherms and researchers classically use either static (exposure to a constant temperature) or dynamic (ramping assays assess tolerance. The different methods complicates comparison between studies here we present mathematical model (and R -scripts) reconcile thermal measures obtained from assays. Our uses input data several experiments based on well-supported assumption that injury accumulation rate increases...

Abstract Ectotherm thermal tolerance is critical to species distribution, but at present the physiological underpinnings of heat remain poorly understood. Mitochondrial function perturbed critically high temperatures in some ectotherms, including insects, suggesting that these animals linked failure oxidative phosphorylation (OXPHOS) and/or ATP production. To test this hypothesis we measured mitochondrial oxygen consumption rates six Drosophila with different using high-resolution...

Abstract The ability of ectothermic animals to live in different thermal environments is closely associated with their capacity maintain physiological homeostasis across diurnal and seasonal temperature fluctuations. For chill-susceptible insects, such as Drosophila , cold tolerance tightly linked ion water obtained through a regulated balance active passive transport. Active transport at low requires constant delivery ATP we therefore hypothesize that cold-adapted are characterized by...