A5063313488- Cancer Immunotherapy and Biomarkers
- Head and Neck Cancer Studies
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- CAR-T cell therapy research
- Salivary Gland Tumors Diagnosis and Treatment
- Immune cells in cancer
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Colorectal and Anal Carcinomas
- Head and Neck Surgical Oncology
- Nanoplatforms for cancer theranostics
- Thyroid Cancer Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Thyroid and Parathyroid Surgery
- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Galectins and Cancer Biology
- Ear and Head Tumors
- vaccines and immunoinformatics approaches
Massachusetts General Hospital
2016-2025
Harvard University
2016-2025
Center for Systems Biology
2021-2025
Dana-Farber/Harvard Cancer Center
2025
Yale University
2010-2024
Yale Cancer Center
2024
Johns Hopkins University
2007-2023
Broad Institute
2023
Lancaster General Hospital
2023
Massachusetts Eye and Ear Infirmary
2021
BRAF V600E is a prominent oncogene in papillary thyroid cancer (PTC), but its role PTC-related patient mortality has not been established.To investigate the relationship between mutation and mortality.Retrospective study of 1849 patients (1411 women 438 men) with median age 46 years (interquartile range, 34-58 years) an overall follow-up time 33 months 13-67 months) after initial treatment at 13 centers 7 countries 1978 2011.Patient deaths specifically caused by PTC.Overall, was 5.3%...
Dendritic cells (DCs), unique antigen-presenting (APCs) with potent T cell stimulatory capacity, direct the activation and differentiation of by providing costimulatory signals. As such, they are critical regulators both natural vaccine-induced immune responses. A new B7 family member, B7-DC, whose expression is highly restricted to DCs, was identified among a library genes differentially expressed between DCs activated macrophages. B7-DC fails bind B7.1/2 receptors CD28 cytotoxic...
Human papillomavirus-associated head and neck squamous cell carcinomas (HPV-HNSCC) originate in the tonsils, major lymphoid organ that orchestrates immunity to oral infections. Despite its location, virus escapes immune elimination during malignant transformation progression. Here, we provide evidence for role of PD-1:PD-L1 pathway HPV-HNSCC resistance. We show membranous expression PD-L1 tonsillar crypts, site initial HPV infection. In HPV-HNSCCs are highly infiltrated with lymphocytes, on...
To investigate the prognostic value of BRAF V600E mutation and recently identified TERT promoter chr5:1,295,228C>T (C228T), individually in their coexistence, papillary thyroid cancer (PTC).We performed a retrospective study relationship C228T mutations with clinicopathologic outcomes PTC 507 patients (365 women 142 men) age 45.9 ± 14.0 years (mean SD) median follow-up 24 months (interquartile range, 8 to 78 months).Coexisting were more commonly associated high-risk characteristics than they...
Mutations 1 295 228 C>T and 250 (termed C228T C250T respectively), corresponding to −124 −146 from the translation start site in promoter of telomerase reverse transcriptase ( TERT ) gene, have recently been reported human cancers, but not thyroid cancers yet. We explored these mutations by genomic sequencing a large number primary tumor samples. found mutation 0 85 (0.0%) benign tumors, 30 257 (11.7%) papillary (PTC), 9 79 (11.4%) follicular (FTC), 3 8 (37.5%) poorly differentiated...
: Cystic lymph node metastases have been associated with tonsil cancer. A subset of oropharyngeal cancers contain human papillomavirus (HPV) DNA. The clinical and virologic associations cystic nodal metastasis in head neck cancer (HNSCC) were investigated.: retrospective review patients undergoing dissection between 2002 2004 identified metastases. Clinical pathologic information was recorded. presence HPV DNA analyzed using situ hybridization a patients.: One hundred dissections reviewed 20...
High-risk human papillomavirus (HPV) is an established cause of head and neck carcinomas arising in the oropharynx. The presence HPV has also been reported some sinonasal tract, but little known about their overall incidence or clinicopathologic profile. surgical pathology archives Johns Hopkins Hospital were searched for all tract from 1995 to 2011, tissue microarrays constructed. p16 immunohistochemical analysis DNA situ hybridization high-risk types performed. Demographic clinical outcome...
Tumor microenvironments (TMEs) influence cancer progression but are complex and often differ between patients. Considering that microenvironment variations may reveal rules governing intratumoral cellular programs disease outcome, we focused on tumor-to-tumor variation to examine 52 head neck squamous cell carcinomas. We found macrophage polarity-defined by
DNA vaccination is an attractive approach for tumor immunotherapy because of its stability and simplicity delivery. Advances demonstrate that helper T cell responses play a critical role in initiating immune responses. The aim the current study to test whether targeting HPV-16 E7 endosomal/lysosomal compartment can enhance potency vaccines. We linked lysosome-associated membrane protein 1 (LAMP-1) HPV-E7 construct chimeric DNA, Sig/E7/LAMP-1 DNA. For vivo prevention experiments, mice were...
Human papillomavirus (HPV)-related carcinomas of the head and neck are characterized by a predilection for oropharynx, nonkeratinizing squamous morphology, infection with HPV 16 type; but comprehensive testing across all sites has shown that pathologic features HPV-related carcinoma may be more wide ranging than initially anticipated. In particular, subset sinonasal positive, these include variant is histologically similar to adenoid cystic (ACC). Cases were identified retrospective...
To better understand oral human papillomavirus (HPV) infection and cancer risk among long-term sexual partners of patients with HPV-positive oropharyngeal (HPV-OPC).An rinse sample, factor survey, history, examination (partners only) were collected from HPV-OPC their partners. Oral samples evaluated for 36 types HPV DNA using PGMY 09/11 primers line-blot hybridization HPV16 copy number quantitative polymerase chain reaction. prevalence was compared those age 45 to 65 years National Health...
The human papillomavirus (HPV) has been identified as a causative factor in 20% to 25% of all head and neck squamous cell carcinomas (HNSCC). Ongoing research suggests that the presence HPV DNA HNSCC predicts positive prognosis with respect disease-free overall survival. However, most studies have limited by heterogeneity treatment regimens and/or anatomic subsites tumor origin. In this study, we correlate clinical outcomes status for patients oropharyngeal who were uniformly treated...
6011 Background: The PD-1 receptor-ligand pathway can be used by tumors to evade immune surveillance, thereby allowing neoplastic growth. MK-3475 is a highly selective, humanized IgG4/kappa isotype mAb designed block interaction with its ligands PD-L1 and PD-L2, reactivate the system eradicate host tumor. Methods: Pts recurrent/metastatic H/N cancer were enrolled in this multi-center, non-randomized trial two cohorts (HPV non-HPV associated). prescreened for expression immunohistochemistry...
Oral tongue squamous cell carcinoma (OTSCC) is the most common oral cavity tumor. In this study, we examined basis for activity of programmed death protein (PD-1)-based immune checkpoint therapy that being explored widely in head and neck cancers. Using multispectral imaging, systematically investigated OTSCC tumor microenvironment (TME) by evaluating frequency PD-1 expression CD8+, CD4+, FoxP3+ tumor-infiltrating lymphocytes (TIL). We also defined cellular sources ligand (PD-L1) to evaluate...
Resident macrophages and recruited neutrophils cause toxicities in tumor-free tissues after immunotherapy.