A5032487642- Thyroid Cancer Diagnosis and Treatment
- Cervical Cancer and HPV Research
- Parasitic Infections and Diagnostics
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Advanced Radiotherapy Techniques
- Head and Neck Cancer Studies
- Amoebic Infections and Treatments
- Cancer Genomics and Diagnostics
- BRCA gene mutations in cancer
- Atomic and Molecular Physics
- Salivary Gland Tumors Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Nutrition, Genetics, and Disease
- Antimicrobial Peptides and Activities
- Radiation Therapy and Dosimetry
- HER2/EGFR in Cancer Research
- Thyroid and Parathyroid Surgery
- Drug Transport and Resistance Mechanisms
- Head and Neck Anomalies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Telomeres, Telomerase, and Senescence
- Laser-Plasma Interactions and Diagnostics
- Melanoma and MAPK Pathways
- Colorectal Cancer Screening and Detection
Johns Hopkins University
2005-2023
Johns Hopkins Medicine
2003-2023
University of New Mexico
1990-2021
Mount Sinai Hospital
2013-2016
University of Vermont
2013-2016
Massachusetts General Hospital
2013-2016
Virginia Commonwealth University
2013-2016
John Wiley & Sons (United States)
2016
Hudson Institute
2016
University Health Network
2016
BRAF V600E is a prominent oncogene in papillary thyroid cancer (PTC), but its role PTC-related patient mortality has not been established.To investigate the relationship between mutation and mortality.Retrospective study of 1849 patients (1411 women 438 men) with median age 46 years (interquartile range, 34-58 years) an overall follow-up time 33 months 13-67 months) after initial treatment at 13 centers 7 countries 1978 2011.Patient deaths specifically caused by PTC.Overall, was 5.3%...
Multidrug resistance in human KB carcinoma cells selected for to colchicine, vinblastine, or adriamycin results from overexpression, and frequently amplification, of a specific gene ( m d r l ) .Overlapping cDNA clones representing complete 4.7-kilobase mdrl transcript have been obtained multidrug-resistant cells.Primer extension S1 nuclease protection experiments show that two transcripts initiate 136 140 bases upstream the first ATG codon all cell lines.The is expressed normal kidney HepG2...
On October 22 and 23, 2007, the National Cancer Institute (NCI) hosted The NCI Thyroid Fine Needle Aspiration (FNA) State of Science Conference, a two-day gathering in Bethesda, Maryland. Its mission was to establish comprehensive interdisciplinary informational dialogue dedicated thyroid FNA. Preparations for conference began 18 months earlier with designation steering committee, appointment nine working committees, coordination co-sponsoring organizations, establishment permanent web site....
Human biospecimens are subjected to collection, processing, and storage that can significantly alter their molecular composition consistency. These biospecimen preanalytical factors, in turn, influence experimental outcomes the ability reproduce scientific results. Currently, extent type of information specific conditions reported publications regulatory submissions varies widely. To improve quality research uses human tissues, it is crucial on handling be a thorough, accurate, standardized...
NKX3.1 is a prostatic tumor suppressor gene located on chromosome 8p. Although most studies have shown that staining for protein positive in the majority of primary adenocarcinomas, it has been to be downregulated many high-grade prostate cancers, and completely lost metastatic cancers (eg, 65% 78% lesions). A recent study showed with novel antibody was highly sensitive specific adenocarcinoma when compared urothelial carcinoma. This raised question this may perform better than earlier used...
Hepatocellular carcinoma (HCC) is one of the most fatal human malignancies, but molecular mechanisms hepatocarcinogenesis remain unclear. Although p53 mutations are frequently observed in Asian HCC, it not a common event Western HCC. Recent studies suggest that tumor suppressor genes (TSGs) can also be silenced through epigenetic disruption, such as promoter CpG island methylation, during carcinogenesis. To further understand mechanism hepatocarcinogenesis, we have investigated methylation...
The MDR1 gene, responsible for multidrug resistance in human cells, encodes a broad specificity efflux pump (P-glycoprotein). P-glycoprotein consists of two similar halves, each half including hydrophobic transmembrane region and nucleotide-binding domain. On the basis sequence homology between N-terminal C-terminal halves P-glycoprotein, we have previously suggested that this gene arose by duplication primordial gene. We now determined complete intron/exon structure direct sequencing cosmid...
This study investigated the utility of BRAF mutation testing thyroid fine-needle aspiration biopsy (FNAB) specimens for preoperative risk stratification in papillary cancer (PTC).We assessed T1799A status FNAB obtained from 190 patients before thyroidectomy PTC and its association with clinicopathologic characteristics tumor revealed postoperatively.We observed a significant poorer outcomes PTC. In comparison wild-type allele, strongly predicted extrathyroidal extension (23% v 11%; P =...
Abstract Purpose: Advances in targeted lung cancer therapy now demand accurate classification of non–small cell (NSCLC). MicroRNAs (miRNA) are recently discovered short, noncoding genes that play essential roles tissue differentiation during normal development and tumorigenesis. For example, hsa-miR-205 is a miRNA highly expressed squamous carcinomas (SqCC) but not adenocarcinomas. The differential expression miRNAs could be exploited to distinguish these tumor types. Experimental Design:...
Abstract Background: Fine needle aspiration (FNA) is routinely used in the preoperative evaluation of thyroid nodules, but subsequent patient management often complicated by inability to decisively recognize malignancy on cytologic grounds alone. Activating mutations BRAF oncogene commonly occur papillary carcinomas (PTCs) not other types benign and malignant lesions. Mutational analysis FNAs could enhance selection nodules for surgical removal. Methods: Ninety-five excised PTCs along with...
We report the generation of laser pulse energies up to $30\ensuremath{\mu}\mathrm{J}$ at 46.9 nm in plasma column a fast compressional capillary discharge. Double pass experiments using an iridium mirror achieved gain-length product greater than 25. The results provide first clear evidence saturated operation table-top soft x-ray amplifier.
Abstract Human biospecimens are subjected to collection, processing, and storage that can significantly alter their molecular composition consistency. These biospecimen preanalytical factors, in turn, influence experimental outcomes the ability reproduce scientific results. Currently, extent type of information specific conditions reported publications regulatory submissions varies widely. To improve quality research uses human tissues, it is crucial on handling be a thorough, accurate,...
Human papillomavirus (HPV) is responsible for the rising incidence of oropharyngeal squamous cell cancers (OSCC) in United States, and yet, no screening strategies have been evaluated. Secondary prevention by means HPV detection cervical cytology has led to a decline cancer States. Here, we explored an analogous strategy evaluating associations between HPV16 infection, cytopathology, histopathology two populations at elevated risk OSCCs. In first, cross-sectional study population (PAP1),...
To determine if focal 'nuclear atypia' or 'microfollicular architecture' portends a higher risk of malignancy than other subcategories atypia undetermined significance (AUS) in thyroid fine-needle aspirations (FNAs).The frequencies The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) categories were calculated from 3,956 FNAs interpreted over 26-month period at Johns Hopkins Hospital after adoption TBSRTC. TBSRTC criteria applied strictly. malignancy, specifically AUS...
Human biospecimens are subject to a number of different collection, processing, and storage factors that can significantly alter their molecular composition consistency. These biospecimen preanalytical factors, in turn, influence experimental outcomes the ability reproduce scientific results. Currently, extent type information specific conditions reported publications regulatory submissions varies widely. To improve quality research utilizing human tissues, it is critical regarding handling...
Breast cancer is the most common malignant disease worldwide, with over 2.26 million new cases in 2020. Its diagnosis determined by a histological review of breast biopsy specimens, which can be labor-intensive, subjective, and error-prone. Artificial Intelligence (AI)-based tools support detection classification biopsies ensuring rapid, accurate, objective diagnosis. We present here development, external clinical validation, deployment routine use an AI-based quality control solution for...
ABSTRACT A growing body of evidence suggests that endogenous antibiotics contribute to the innate defense mammalian mucosal surfaces. In cow, β-defensins constitute a large family antibiotic peptides whose members have been previously isolated from respiratory and oral mucosa, as well circulating phagocytic cells. novel bovine genomic clone with sequence related those these α-defensins was characterized. The corresponding cDNA small intestinal library; its open reading frame predicts deduced...
Antimicrobial peptides comprise a diverse class of molecules used in host defense by plants, insects, and animals.In this study we have isolated novel antimicrobial peptide from the skin bullfrog, Rana catesbeiana.This 20 amino acid peptide, which termed Ranalexin, has sequence: NH,-Phe-Leu-Gly-Gly-Leu-Ile-Lys-Ile-Val-Pro-Ala-Met-Ile-Cys-Ala-Val-Thr-Lys-Lys-Cys-COOH, it contains
Although 10%-15% of patients with AIDS in the United States may acquire cryptosporidium infection, little data exist on clinical or histological characteristics that differentiate outcomes. A case-control study 83 HIV-positive adult cryptosporidiosis was conducted, as a histopathologic review gastrointestinal biopsy specimens from 30 patients. Four syndromes were identified: chronic diarrhea (36% patients), choleralike disease (33%), transient (15%), and relapsing illness (15%). multivariate...
Objective: To characterize the histology of AIDS-associated cryptosporidiosis and identify features that explain clinical variability. Design: A retrospective analysis HIV-positive individuals with who underwent endoscopy at Johns Hopkins Hospital between 1985 1996. Methods: The histologic (intensity Cryptosporidium infection, inflammation, mucosal damage, copathogens) gastrointestinal biopsies from 37 were systematically graded. These correlated severity diarrheal illness obtained a patient...
a) To assess the age-related incidence of morbid cardiac events including death (CD), nonfatal myocardial infarction (MI), and angina pectoris (AP) in all patients treated for Hodgkin's disease at a single institution; b) to examine prevalence risk factors presence coronary artery (CAD) affected patients.475 were our institution between 1954 1989. The status 97% cohort was established either by patient visit examination 1992-1993, personal telephone contact, or documentation death. 326 these...