Shani T. Gal-Oz
A5067649208- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Reproductive System and Pregnancy
- Hippo pathway signaling and YAP/TAZ
- Congenital heart defects research
- Sex and Gender in Healthcare
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Immune cells in cancer
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Science Education and Perceptions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Single-cell and spatial transcriptomics
- Biomedical and Engineering Education
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- Social Media in Health Education
Ben-Gurion University of the Negev
2018-2024
Brigham and Women's Hospital
2023-2024
Harvard University
2023-2024
La Jolla Institute for Immunology
2021
Abstract Neutrophils are implicated in multiple homeostatic and pathological processes, but whether functional diversity requires discrete neutrophil subsets is not known. Here, we apply single-cell RNA sequencing to neutrophils from normal inflamed mouse tissues. Whereas conventional clustering yields alternative organizational structures, diffusion mapping plus velocity discloses a single developmental spectrum, ordered chronologically. Termed here neutrotime, this spectrum extends...
Abstract Sexual dimorphism in the mammalian immune system is manifested as more frequent and severe infectious diseases males and, on other hand, higher rates of autoimmune disease females, yet insights underlying those differences are still lacking. Here we characterize sex by RNA ATAC sequence profiling untreated interferon-induced cell types male female mice. We detect very few differentially expressed genes between cells except macrophages from three different tissues. Accordingly,...
"γc" cytokines are a family whose receptors share "common-gamma-chain" signaling moiety, and play central roles in differentiation, homeostasis, communications of all immunocyte lineages. As resource to better understand their range specificity action, we profiled by RNAseq the immediate-early responses main γc across The results reveal an unprecedented landscape: broader, with extensive overlap between (one cytokine doing one cell what another does elsewhere) essentially no effects unique...
Significance In this work, we show that the last step of cytokinesis, termed abscission, is delayed in early zebrafish embryos. As a result, sibling cells remain connected to one another by thin membrane bridge for several cycles, forming clusters interconnected cells. Bridge severing (i.e., abscission) commences at 10th cell cycle when embryos enter midblastula transition switch, which embryonic become individualized and exhibit characteristics mature Cells intercellular bridges shared...
The response to type I IFNs involves the rapid induction of prototypical IFN signature genes (ISGs). It is not known whether tightly controlled ISG expression observed at cell population level correctly represents coherent responses individual cells or it masks some heterogeneity in gene modules and/or responding cells. We performed a time-resolved single-cell analysis first 3 h after vivo stimulation macrophages and CD4+ T B lymphocytes from mice. All ISGs were generally induced concert,...
Fibroblasts play critical roles in tissue homeostasis, but pathologic states they can drive fibrosis, inflammation, and destruction. Little is known about what regulates the homeostatic functions of fibroblasts. Here, we perform RNA sequencing identify a gene expression program healthy synovial fibroblasts characterized by enhanced fatty acid metabolism lipid transport. We cortisol as key driver fibroblast phenotype that depletion adipocytes, which express high levels Hsd11b1, results loss...
Fibroblasts play critical roles in tissue homeostasis, but pathologic states can drive fibrosis, inflammation, and destruction. In the joint synovium, fibroblasts provide homeostatic maintenance lubrication. Little is known about what regulates functions of healthy conditions. We performed RNA sequencing human synovial identified a fibroblast gene expression program characterized by enhanced fatty acid metabolism lipid transport. found that fat-conditioned media reproduces key aspects...
Abstract Alternative splicing results in multiple transcripts of the same gene, possibly encoding for different protein isoforms with domains. Whereas it is possible to manually determine effect alternative on domain composition a single event, process requires tedious integration several data sources; error prone and not feasible genome-wide characterization domains affected by differential splicing. To fulfill need an automated solution, we developed Domain Change Presenter (DoChaP,...
ABSTRACT Animal cytokinesis ends with the formation of a thin intercellular membrane bridge connecting two newly formed sibling cells that is ultimately resolved by abscission. While mitosis completed within 15 minutes, can persist for hours, maintaining physical connection between and allowing exchange cytosolic components. Although cell-cell communication fundamental development, potential role bridges during embryogenesis have not been fully elucidated. Here, we found in early zebrafish (...
Abstract Alternative RNA splicing results in multiple transcripts of the same gene, possibly encoding for different protein isoforms with domains and functionalities. Whereas it is possible to manually determine effect a specific alternative event on domain composition particular encoded protein, process requires tedious integration several data sources; therefore error prone its implementation not feasible genome-wide characterization affected by differential splicing. To fulfill need an...