A5046293336- Nephrotoxicity and Medicinal Plants
- Virus-based gene therapy research
- Immune cells in cancer
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Rabies epidemiology and control
- Drug-Induced Hepatotoxicity and Protection
- Bladder and Urothelial Cancer Treatments
- interferon and immune responses
- RNA regulation and disease
- Hepatitis B Virus Studies
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Heavy Metals in Plants
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- Cell Adhesion Molecules Research
- Virology and Viral Diseases
- Cancer Cells and Metastasis
- Animal Disease Management and Epidemiology
- Renal cell carcinoma treatment
- Multiple and Secondary Primary Cancers
Institut Curie
2018-2024
Inserm
2018-2023
Université Paris Sciences et Lettres
2019-2022
Immunité et Cancer
2022
Institut de Biologie Intégrative de la Cellule
2016-2019
Centar za Promociju Nauke
2011-2019
Centre National de la Recherche Scientifique
2014-2018
CEA Paris-Saclay
2014-2018
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2018
Université Paris-Saclay
2014-2018
Replication of Mononegavirales occurs in viral factories which form inclusions the host-cell cytoplasm. For rabies virus, those are called Negri bodies (NBs). We report that NBs have characteristics similar to liquid organelles: they spherical, fuse larger structures, and disappear upon hypotonic shock. Their phase is confirmed by FRAP experiments. Live-cell imaging indicates nucleocapsids ejected from transported along microtubules either new virions or secondary factories. Coexpression...
Vesicular stomatitis virus (VSV) is an oncolytic rhabdovirus and its glycoprotein G widely used to pseudotype other viruses for gene therapy. Low-density lipoprotein receptor (LDL-R) serves as a major entry VSV. Here we report two crystal structures of VSV in complex with distinct cysteine-rich domains (CR2 CR3) LDL-R, showing that their binding sites on are identical. We identify basic residues G, which essential interaction CR2 CR3. Mutating these abolishes infectivity even though can use...
Stress granules (SGs) are membrane-less dynamic structures consisting of mRNA and protein aggregates that form rapidly in response to a wide range environmental cellular stresses viral infections. They act as storage sites for translationally silenced mRNAs under stress conditions. During infection, SG formation results the modulation innate antiviral immune responses, several viruses have ability either promote or prevent assembly. Here, we show rabies virus (RABV) induces infected cells,...
ABSTRACT The rabies virus (RABV) phosphoprotein P is a multifunctional protein: it plays an essential role in viral transcription and replication, addition, RABV has been identified as interferon antagonist. Here, yeast two-hybrid screen revealed that interacts with the focal adhesion kinase (FAK). binding involved 106-to-131 domain, corresponding to dimerization domain of C-terminal FAK containing proline-rich domains PRR2 PRR3. P-FAK interaction was confirmed infected cells by...
DNA adducts represent internal dosimeters to measure exposure environmental and endogenous genotoxicants. Unfortunately, in molecular epidemiologic studies, measurements of often are precluded by the unavailability fresh tissue. In contrast, formalin-fixed paraffin embedded (FFPE) tissues frequently accessible for biomarker discovery. We report here that aristolochic acids (AAs) can be measured FFPE at a level sensitivity comparable freshly frozen AAs nephrotoxic carcinogenic compounds found...
In innate immune cells, intracellular sensors such as cGAS-STING stimulate type I/III interferon (IFN) expression, which promotes antiviral defense and activation. However, how IFN-I/III expression is controlled in adaptive cells poorly understood. Here, we identify a transcriptional rheostat orchestrated by RELA that confers human T with innate-like abilities to produce IFN-I/III. Despite intact signaling, responses are stunted CD4+ compared dendritic or macrophages. We find lysine residues...
ABSTRACT Rabies virus replicates in the cytoplasm of host cells, but rabies phosphoprotein (P-protein) undergoes active nucleocytoplasmic trafficking. Here we show that largely nuclear P-protein isoform P3 can localize to nucleoli and forms specific interactions with nucleolin. Importantly, depletion nucleolin expression inhibits viral protein infectious production by infected cells. This provides first evidence lyssaviruses interact is important lyssavirus infection.
DNA adducts are a measure of internal exposure to genotoxicants. However, the measurement in molecular epidemiology studies often is precluded by lack fresh tissue. In contrast, formalin-fixed paraffin-embedded (FFPE) tissues frequently accessible, although technical challenges remain retrieval high quality suitable for biomonitoring adducts. Aristolochic acids (AA) human carcinogens found Aristolochia plants, some which have been used preparation traditional Chinese herbal medicines. We...
The aim of the study was to investigate upper urothelial tumors (UUT) in emigrants from Balkan endemic nephropathy (BEN) areas Serbia and compare them with UUT both nonendemic areas.A total 1,121 patients state cancer database, between 1960 1998, were investigated. Sixty BEN areas.UUT occurred after 21.7+/-9.9 years (median 20) spent a rural environment. time outside regions 33.2+/-12.8 31, range 10-72). Age at surgery 55 (range 31-89). In areas, there significant association other diseases...
Previously we reported that the proportion of Ig kappa to lambda anti-DNP antibodies produced by day 5 a primary response thymus-dependent antigens was fivefold greater than kappa:Ig ratios found in responses thymus-independent antigens. To determine whether an affinity-based process explained differences kappa/lambda seen, measured affinity and antibody using binding ratio radioimmunoassay method, which measures particular isotypes without need for prior purification. By addition mild...
<title>Abstract</title> While extensive research has unraveled diverse biochemical mechanisms underlying the pro-tumorigenic activities of macrophages, role physical forces in early tumor development remains elusive. Here, we integrated experimental data with modeling to investigate contribution various forces. Monitoring growth KrasG12Dp53-/- (KP) lung spheroids vitro 3 dimensions using real-time microscopy accurately reflects tissue-resident rather than monocytes, promoting growth. Based...
In order to evaluate the immune infiltrate of AT/RT, we conducted a combined immunohistochemical, multiparametric flow cytometry and transcriptomic analysis series 49 human AT/RT. This revealed substantial heterogeneity, with some subgroups AT/RT showing prominent infiltrate. details, our analyses indicated that: i) myeloid cells were most abundant population, including both microglial non-resident pro-tumoral M2-polarized macrophages, ii) tumor-infiltrating lymphocytes consisted in equal...