A5049012918- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Hemoglobinopathies and Related Disorders
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- RNA Research and Splicing
- Algal biology and biofuel production
- Dermatology and Skin Diseases
- Erythropoietin and Anemia Treatment
- Infectious Diseases and Mycology
- Biological Activity of Diterpenoids and Biflavonoids
- Mycobacterium research and diagnosis
- Antifungal resistance and susceptibility
- Circular RNAs in diseases
- Wastewater Treatment and Nitrogen Removal
- Polyamine Metabolism and Applications
- Nonmelanoma Skin Cancer Studies
- Blood groups and transfusion
- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Glycosylation and Glycoproteins Research
- Blood properties and coagulation
New York Blood Center
2015-2024
Northwestern Polytechnical University
2024
Jining First People's Hospital
2014-2024
Georgetown University Medical Center
2024
Georgetown University
2024
Weifang Medical University
2024
Inner Mongolia International Mongolian Hospital
2020-2023
Inner Mongolia People's Hospital
2023
Donghua University
2023
Wuhan Dongxihu District People Hospital
2023
Highlights•Epigenomic landscape of erythropoiesis reveals stage-specific patterns regulation•Epigenomic changes in are linked to erythroid traits and disease genes•Erythroid cells exhibit chromatin accessibility distinct from other cell typesSummaryMammalian yields a highly specialized type, the mature erythrocyte, evolved meet organismal needs increased oxygen-carrying capacity. To better understand regulation erythropoiesis, we performed genome-wide studies accessibility, DNA methylation,...
Fine-resolution differentiation trajectories of adult human hematopoietic stem cells (HSCs) involved in the generation red is critical for understanding dynamic developmental changes that accompany erythropoiesis. Using single-cell RNA sequencing (scRNA-seq) primary terminal erythroid (CD34 − CD235a + ) isolated directly from bone marrow (BM) and umbilical cord blood (UCB), we documented transcriptome terminally differentiated erythroblasts at unprecedented resolution. The insights enabled...
The metabolic changes controlling the stepwise differentiation of hematopoietic stem and progenitor cells (HSPCs) to mature erythrocytes are poorly understood. Here, we show that HSPC development an erythroid-committed proerythroblast results in augmented glutaminolysis, generating alpha-ketoglutarate (αKG) driving mitochondrial oxidative phosphorylation (OXPHOS). However, sequential late-stage erythropoiesis is dependent on decreasing αKG-driven OXPHOS, find isocitrate dehydrogenase 1...
Abstract Studies of human erythropoiesis have relied, for the most part, on in vitro differentiation hematopoietic stem and progenitor cells (HSPC) from different sources. Here, we report that despite common core erythroid program exists between cord blood (CB)‐ peripheral (PB)‐HSPC induced toward vitro, significant functional differences exist. We undertook a comparative analysis using these two sources HSPC. Upon differentiation, CB‐derived proliferated 4‐fold more than PB‐derived cells....
While ineffective erythropoiesis has long been recognized as a key contributor to anemia in thalassemia, its role of sickle cell disease (SCD) not critically explored. Using vitro and vivo derived human erythroblasts we assessed the extent SCD. Modeling bone marrow hypoxic environment, found that hypoxia induces death starting at polychromatic stage, positively selecting cells with high levels fetal hemoglobin (HbF). Cell was associated cytoplasmic sequestration heat shock protein 70 rescued...
Identification of stage-specific erythroid cells is critical for studies normal and disordered human erythropoiesis. While immunophenotypic strategies have previously been developed to identify at each stage terminal differentiation, progenitors are currently defined very broadly. Refined characterize BFU-E CFU-E subsets critically needed. To address this unmet need, a flow cytometry-based technique was that combines the established surface markers CD34 CD36 with CD117, CD71, CD105. This...
SF3B1 is a core component of splicing machinery. Mutations in are frequently found myelodysplastic syndromes (MDS), particularly patients with refractory anemia ringed sideroblasts (RARS), characterized by isolated anemia. mutations have been implicated the pathophysiology RARS; however, physiological function erythropoiesis remains unknown. shRNA-mediated approach was used to knockdown human CD34+ cells. The effects on erythroid cell differentiation, cycle, and apoptosis were assessed flow...
Despite the effective clinical use of steroids for treatment Diamond Blackfan anemia (DBA), mechanisms through which glucocorticoids regulate human erythropoiesis remain poorly understood. We report that sensitivity erythroid differentiation to dexamethasone is dependent on developmental origin CD34+ progenitor cells, specifically increasing expansion progenitors from peripheral blood (PB) but not cord (CB). Dexamethasone erythroid-differentiated PB, CB, resulted in a newly defined...
This study aims to illustrate epidemiology of comorbid CVD in the real-world clinical setting patients with psoriasis China. We used data adult who were registered register China National Clinical Center for Skin and Immune Diseases between August 2020 September 2021. Psoriasis was clinically diagnosed following national guidelines. Univariate multivariate logistic regression models examine factors associated psoriasis. Of 11,560 (age ≥ 18 years, mean age 41.87 64.88% males), 236 ascertained...
In malignant melanoma, tumor-associated macrophages play multiple roles in promoting tumor growth, such as inducing the transformation of melanocytes under ultraviolet irradiation, increasing angiogenesis melanomas, and suppressing antitumor immunity. Because granzyme B- perforin-expressing Tr1 cells could specifically eliminate antigen-presenting myeloid origin, we examined whether melanoma tumor-promoting how interaction between affect growth cells. were characterized by high interleukin...
Dendritic cells (DCs) are known to play an important role in initiating and orchestrating antimicrobial immunity. Given the fact that candidiasis appears often immunocompromised patients, it seems plausible DCs hold key new antifungal strategies. One possibility enhance potency of DC-based immunotherapy is silence negative immunoregulatory pathways through ablation suppressor cytokine signaling 1 (SOCS1). Here, we deliver small interfering RNA (siRNA) against SOCS1 into murine bone marrow...
A 58-year-old man who lived in northern China presented to the dermatology clinic with an asymptomatic cutaneous lesion on his right forearm that had been present for at least a month. He was shepherd, and reported some of sheep developed lesions around their mouths 6 weeks