A5031159776- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
- Blood properties and coagulation
- Advanced Glycation End Products research
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Blood groups and transfusion
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Blood disorders and treatments
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Acute Myeloid Leukemia Research
- Lymphatic System and Diseases
- Wound Healing and Treatments
- Barrier Structure and Function Studies
- Neonatal Health and Biochemistry
- Immune cells in cancer
- Selenium in Biological Systems
- Vascular Malformations and Hemangiomas
- Retinal Diseases and Treatments
University of Pennsylvania
2022-2024
Children's Hospital of Philadelphia
2022-2024
Philadelphia University
2024
Feinstein Institute for Medical Research
2015-2022
Hofstra University
2013-2022
Institute of Molecular Medicine
2022
Cohen Children's Medical Center
2021
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2018-2020
Tufts University
2012-2015
Center for Innovation
2014-2015
Microvascular stability and regulation of capillary tonus are regulated by pericytes their interactions with endothelial cells (EC). While the RhoA/Rho kinase (ROCK) pathway has been implicated in modulation pericyte contractility, part via myosin light chain phosphatase (MLCP), mechanisms linking Rho GTPase activity actomyosin-based contraction cytoskeleton equivocal. Recently, phosphatase-RhoA-interacting protein (MRIP) was shown to mediate RhoA/ROCK-directed MLCP inactivation vascular...
Despite the effective clinical use of steroids for treatment Diamond Blackfan anemia (DBA), mechanisms through which glucocorticoids regulate human erythropoiesis remain poorly understood. We report that sensitivity erythroid differentiation to dexamethasone is dependent on developmental origin CD34+ progenitor cells, specifically increasing expansion progenitors from peripheral blood (PB) but not cord (CB). Dexamethasone erythroid-differentiated PB, CB, resulted in a newly defined...
Purpose.: To establish the regulatory roles that pericytes have in coordinating retinal endothelial cell (EC) growth and angiogenic potential. Methods.: Pericytes were derived from donor diabetic (DHuRP) or normal (NHuRP) human retinae, characterized using vascular markers, coculture, contraction, morphogenesis, proliferation assays. investigate capillary "cross-talk," pericyte-endothelial coculture growth, connexin-43 (Cx43) expression assays performed. Paracrine effects examined via...
Abstract Despite effective clinical use, the mechanistic bases for regulation of human erythropoiesis by glucocorticoids remain poorly understood. Here, we employed an erythroid culture system to differentiate primary CD34 + cells isolated from control peripheral blood, cord blood and patients with Diamond Blackfan anemia (DBA), as model erythropoietic disorder treated glucocorticoids. We found that response Dexamethasone is dependent on developmental origin source cells, specifically...
Abstract Ribosomopathies are a class of disorders caused by defects in the structure or function ribosome and characterized tissue-specific abnormalities. Diamond Blackfan anemia (DBA) arises from different mutations, predominantly genes encoding ribosomal proteins (RPs). Apart anemia, skeletal among most common anomalies observed patients with DBA, but they virtually restricted to radial ray other upper limb defects. What leads these site-specific DBA remains mystery. Using novel mouse...
Abstract Background: Diamond Blackfan anemia (DBA) is an inherited bone marrow failure syndrome characterized by red cell aplasia and congenital anomalies, notably skeletal defects. DBA known in the majority of cases to be caused haploinsufficiency one small or large ribosomal protein (rp) subunits. In addition, there a cancer predisposition DBA. Indeed, somatic germline rp mutations have been associated with number malignancies. patients common malignancies osteogenic sarcoma (OS). It...