A5016641678- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Hemoglobinopathies and Related Disorders
- Metabolism and Genetic Disorders
- Platelet Disorders and Treatments
- Cardiac Arrhythmias and Treatments
- Congenital Heart Disease Studies
- Blood disorders and treatments
- Blood donation and transfusion practices
- Immune Cell Function and Interaction
- Adrenal Hormones and Disorders
- Chronic Lymphocytic Leukemia Research
- Neonatal Health and Biochemistry
- Autoimmune Bullous Skin Diseases
- Cardiac Valve Diseases and Treatments
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Amino Acid Enzymes and Metabolism
- Complement system in diseases
- Connective tissue disorders research
- Blood Coagulation and Thrombosis Mechanisms
- Biochemical and Molecular Research
- Liver Disease Diagnosis and Treatment
- Dermatological and Skeletal Disorders
- Heme Oxygenase-1 and Carbon Monoxide
New York Blood Center
2016-2025
Seattle University
2016-2020
Emerald Coast Science Center
2018
Iowa City Public Library
2016
Hudson Institute
2016
John Wiley & Sons (United States)
2016
American Red Cross
2007-2010
Charité - Universitätsmedizin Berlin
2010
Bristol Institute for Transfusion Sciences
2006
Lawrence Berkeley National Laboratory
2006
The development of neutralizing antibodies (NAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) following infection or vaccination is likely to be critical for the sufficient population immunity drive cessation disease 2019 (COVID-19) pandemic. A large number serologic tests, platforms, and methodologies are being employed determine seroprevalence in populations select convalescent plasma samples therapeutic trials guide policies about reopening. However, tests have...
The human colonic mucosa contains regulatory type 1-like (Tr1-like, i.e. IL-10-secreting and Foxp3-negative) T cells specific for the gut Clostridium Faecalibacterium prausnitzii (F. prausnitzii), which are both decreased in Crohn's disease patients. These data, together with demonstration, mice, that (Treg) induced by bacteria key players colon homeostasis, support a similar role F. prausnitzii-specific Treg colon. Here we assessed mechanisms whereby induces Treg. We demonstrated...
Preclinical and clinical studies demonstrate the feasibility of treating β-thalassemia Sickle Cell Disease (SCD) by lentiviral-mediated transfer human β-globin gene. However, previous have not addressed whether ability lentiviral vectors to increase hemoglobin synthesis might vary in different patients.We generated carrying gene with without an ankyrin insulator compared their induce vitro thalassemic mice. We found that insertion leads higher, potentially therapeutic levels through a novel...
T follicular helper cells are the main CD4+ specialized in supporting B-cell responses, but their role driving transfusion-associated alloimmunization is not fully characterized. Reports of subsets displaying various markers and functional activities underscore need for better characterization/identification with defined functions. Here we show that a previously unidentified subset human circulating expressing TIGIT, T-cell immunoreceptor Ig tyrosine-based inhibitory domains, exhibit strong...
Abstract The development of neutralizing antibodies (nAb) against SARS-CoV-2, following infection or vaccination, is likely to be critical for the sufficient population immunity drive cessation COVID19 pandemic. A large number serologic tests, platforms and methodologies are being employed determine seroprevalence in populations select convalescent plasmas therapeutic trials, guide policies about reopening. However, tests have substantial variability sensitivity specificity, their ability...
Engagement of T-cell receptors (TCRs) on immature thymocytes by self-antigen-major histocompatibility complexes causes the death self-reactive via apoptosis, a phenomenon that establishes tolerance. Similarly, treatment with anti-TCR antibodies leads to TCR-mediated which can also be induced in hybridomas. apoptosis and hybridomas requires expression new set genes. In particular, it has recently been shown Nur77, transcription factor is member steroid/thyroid receptor superfamily, required...
Red blood cell alloimmunization is a major complication of transfusion therapy. Host immune markers that can predict antibody responders remain poorly described. As regulatory T cells (Tregs) play role in mouse models, we analyzed the Treg compartment cohort chronically transfused patients with sickle disease (SCD, n = 22) and β-thalassemia (n 8) without alloantibodies. We found reduced activity alloantibody compared nonresponders as seen mice. Higher circulating anti-inflammatory IL-10...
Patients with sickle cell disease (SCD) often require transfusions to treat and prevent worsening anemia other SCD complications. However, can trigger alloimmunization against transfused RBCs serious clinical sequelae. Risk factors for in remain poorly understood. We recently reported altered regulatory T (Treg) Th responses higher circulating Th1 (IFN-γ(+)) cytokines chronically patients alloantibodies as compared those without alloantibodies. Because monocytes play a critical role...
Transfusion therapy is a life-sustaining treatment for patients with sickle cell disease (SCD), but can cause serious complications including alloimmunization. We previously reported diminished regulatory T cells (Tregs) and skewed Th2 responses in alloimmunized SCD patients. hypothesized that the B (Breg) compartment, which controls Treg Th differentiation, may also be compromised allosensitized Phenotypically, we did not find differences frequency or numbers of CD24(hi) CD38(hi) CD27(+)...
Gene therapy for sickle cell disease is limited by the yield of hematopoietic progenitor cells that can be harvested transduction or gene editing. We therefore performed a phase I dose-escalation study mobilizing agent plerixafor to evaluate efficacy and safety standard dosing on peripheral blood CD34+ mobilization. Of 15 patients enrolled date, only one was chronically transfused ten were hydroxyurea. eight who achieved concentration >30 cells/μL, six There no clear dose response increasing...