A5032060710- Monoclonal and Polyclonal Antibodies Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Renal Diseases and Glomerulopathies
- Chronic Lymphocytic Leukemia Research
- Autoimmune Bullous Skin Diseases
- Glycosylation and Glycoproteins Research
- Peripheral Neuropathies and Disorders
- Immunodeficiency and Autoimmune Disorders
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Complement system in diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Hereditary Neurological Disorders
- Erythrocyte Function and Pathophysiology
- Proteoglycans and glycosaminoglycans research
- Multiple Myeloma Research and Treatments
- Vasculitis and related conditions
- Signaling Pathways in Disease
- Clostridium difficile and Clostridium perfringens research
- Chronic Myeloid Leukemia Treatments
- Protein purification and stability
- Diabetes and associated disorders
- Neurogenetic and Muscular Disorders Research
- Space Satellite Systems and Control
Centre National de la Recherche Scientifique
2009-2025
Inserm
2005-2025
Université de Limoges
2010-2025
Contrôle de la Réponse Immune B et Lymphoproliférations
2009-2025
Centre Hospitalier Universitaire de Limoges
2019-2024
Centre Hospitalier Universitaire de Poitiers
2024
Hôpital Dupuytren
2021
Université de Montpellier
2005
École Pratique des Hautes Études
2005
Sequentially along B cell differentiation, the different classes of membrane Ig heavy chains associate with Igα/Igβ heterodimer within receptor (BCR). Whether each class conveys specific signals adapted to corresponding differentiation stage remains debated. We investigated impact forced expression an IgA-class throughout murine by knocking in human Cα gene place Sμ region. Despite a functional BCR, homozygous mutant mice showed partial developmental blockade at pro-B/pre-BI and large...
Background While most antibody-based therapies use IgG because of their well-known biological properties, some functional limitations these antibodies call for the development derivatives with other therapeutic functions. Although less abundant than in serum, IgA is abundantly produced Ig class humans. Besides specific targeting its dimeric form to mucosal areas, was shown recruit polymorphonuclear neutrophils against certain targets more efficiently does IgG1.Design and Methods In this...
IgA1 mesangial deposition is the hallmark of IgA nephropathy and Henoch–Schönlein purpura, onset which often follows infections. Deposited has been reported as polymeric, J chain associated, often, hypogalactosylated but with no information concerning influence repertoire or link between immune stimuli structure. We explored these issues in α 1KI mouse model, produces polyclonal human prone to deposition. Compared mice challenged by a conventional environment, specific pathogen–free...
Abstract B‐cell fate and responses are modulated by soluble mediators direct cellular interactions. Migration properties also vary during differentiation, commitment activation. In many cells, modulation of to stimuli involves cell surface glycans, whose architecture depends on the simultaneous expression multiple enzymes. By looking at glycosylation‐related gene patterns among populations, we determined in this study that strongest variations were observed for CSGalNAcT‐1 EXTL1. These...
The immune response is a key player in the course of SARS-CoV-2 infection, and often seriously dysfunctional severe Coronavirus Disease 2019. hyperinflammatory status has been described to be accompanied by appearance autoantibodies. In lethal COVID-19 we observed emergence de novo natural alloantibody which targeted M antigen from MNS blood group on red cells (RBC) without evidence any cross-reaction with antigens. This IgM lambda was unmutated unswitched. Here, describe for first time...
POEMS syndrome is a rare form of plasma cell dyscrasia with multiple clinical features including polyneuropathy and sclerotic bone lesions. As various signs symptoms can be observed, diagnosis often delayed we need an accurate tool for these patients. Vascular endothelial growth factor (VEGF) known to play major role in the pathology frequently increased sera patients but not specific. This condition also associated presence monoclonal immunoglobulin light chain composed highly restricted...
To understand the fine differential elements that can lead to or prevent acute respiratory distress syndrome (ARDS) in COVID-19 patients, it is crucial investigate immune response architecture. We herein dissected multiple layers of B cell responses by flow cytometry and Ig repertoire analysis from phase recovery. Flow with FlowSOM showed major changes associated inflammation such as an increase double-negative B-cells ongoing plasma differentiation. This paralleled COVID-19-driven expansion...
Abstract Background Differential diagnosis of Waldenström macroglobulinemia (WM) with other indolent B‐cell malignancies is still a challenge. Here, we propose an original and simple analysis routine flow cytometry (FCM) unraveling the characteristic ongoing plasma cell (PC) differentiation WM tumor B‐cells. Methods FCM both B‐cells PC was performed on series 77 patients IgM peak. MYD88 CXCR4 mutations were studied using allele‐specific PCR by high throughput sequencing. Results Twenty seven...
ABSTRACT Plasma cells (PC) represent the last stage of B cell development and are mainly characterized by their capacity secreting large quantities antibodies. They can be implicated in a broad-spectrum neoplastic disorders, including Multiple Myeloma, Waldenstrom macroglobulinemia or Monoclonal Gammopathy Clinical Significance, all abnormal proliferation PC clone. Up to date, there only few reporter models specifically follow development, migration homing mouse none allowing genetic...